Document Type : Original Research
Authors
1 Private Practice, Tehran, Iran
2 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Abstract
Background & Objective: Formaldehyde is an irritating substance that is categorized as a definite carcinogen (Group A1), according to the International Agency for Research on Cancer (IARC). This study was conducted to determine the role of this substance in the frequency of micronuclei (MN) in the buccal mucosal cells due to long-term exposure of the pathology staff to formaldehyde.
Methods: In this case-control study, 32 pathology laboratory staff members were assigned to the case group, and 32 staff members who were not exposed to formaldehyde were assigned to the control group. Buccal mucosa cells were collected with a wet spatula and stained with Papanicolaou stain. In each sample, 500 cells were counted; then, the frequency of MN and the average number of MN in the micronucleated cells were assessed and compared between the 2 groups using the independent t test. Furthermore, the relationship between gender and MN was evaluated using the independent t test. The relationship between years of exposure and time of exposure during the day (in hours) for the case group, as well as the relationship between age and frequency of MN was analyzed using the Pearson correlation coefficient.
Results: The mean frequency of MN in exfoliated buccal cells was 18.33±12.36 in the case group, which was significantly higher than the control group (10.55±6.22; P=0.003). The difference in the mean number of total MN in the micronucleated cells was not significant between the case and control groups (P=0.11). The relationship between sex, age, and years of exposure with the mean frequency of MN and the total number of MN in the micronucleated cells was not significant. The relationship between exposure time during the day and both the mean frequency of MN and the total number of MN in the micronucleated cells was significant (P=0.03).
Conclusion: Formaldehyde exposure and extended time of exposure during the day can increase the frequency of MN, which can prognosticate the incidence of precancerous and cancerous lesions. Therefore, continuous exposure to formaldehyde can be considered an occupational health hazard, though further studies are needed to confirm this result.
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Main Subjects
- Villadiego-Molinares MM, Ramirez-Martinez JA, Rodriguez-Pulido AI. Formaldehyde in occupational environments: literature review and an occupational health surveillance proposal. Rev Fac Med. 2020;68(3):425-37. [DOI:10.15446/revfacmed.v68n3.73188]
- Peteffi GP, da Silva LB, Antunes MV, Wilhelm C, Valandro ET, Glaeser J, et al. Evaluation of genotoxicity in workers exposed to low levels of formaldehyde in a furniture manufacturing facility. Toxicol Ind Health. 2016;32(10):1763-73. [DOI:10.1177/0748233715584250] [PMID]
- Sivasankari NP, Sundarapandian S, Sakthivel E. Micronucleus assay in formalin exposed individuals. Indian J Clin Anat Physiol. 2018;5(1):81-4. [DOI:10.18231/2394-2126.2018.0019]
- Karami Mosafer A, Assari MJ, Bahrami A, Zolhavarie M. Relationship of Ambient Concentrations with Personal Exposure Level of Formaldehyde in the Pathology Departments of Teaching Hospitals Affiliated to Hamadan University of Medical Sciences, Hamadan, Iran. J Occup Hyg Eng. 2017;4(3): 33-40. [DOI:10.21859/johe.4.3.33]
- Lorenzoni DC, Pinheiro LP, Nascimento HS, Menegardo CS, Silva RG, Bautz WG,et al. Could formaldehyde induce mutagenic and cytotoxic effects in buccal epithelial cells during anatomy classes? Med Oral Patol Oral Cir Bucal. 2017; 1;22 (1):e58-63.
- Lin D, Guo Y, Yi J, Kuang D, Li X, Deng H, et al. Occupational exposure to formaldehyde and genetic damage in the peripheral blood lymphocytes of plywood workers. J Occup Health. 2013;55(4):284-91. [DOI:10.1539/joh.12-0288-OA] [PMID]
- Yahyaei E, Majlesi B, Naimi joubani M, Pourbakhshi Y, Ghiyasi S, Jamshidi Rastani M, Heidari M. Occupational Exposure and Risk Assessment of Formaldehyde in the Pathology Departments of Hospitals. Asian Pac J Cancer Prev. 2020; 21 (5): 1303-9. [DOI:10.31557/APJCP.2020.21.5.1303] [PMID]
- Bolognesi C, Fenech M. Micronucleus comet assays in human lymphocytes and buccal cells. Methods MolBiol. 2019;2031:147-163. [DOI:10.1007/978-1-4939-9646-9_8] [PMID]
- Shahsavari F, Ghasemi S, Farhadi S, Mazinani P, Delavari S. Occupational Exposure in Dental Laboratory Technicians May Induce Nuclear Abnormalitiesin Buccal Mucosa Cells: A Preliminary Study. J Res Dentomaxillofac Sci. 2016;1(2):23-7. [DOI:10.29252/jrdms.1.2.23]
- Flores-Garcia A, Torres-Bugarin O, Velarde-Félix JS, Rangel-Villalobos H, Zepeda-Carrillo EA, Rodríguez-Trejo A, et al. Micronuclei and other nuclear anomalies in exfoliated buccal mucosa cells of Mexican women with breast cancer. J BUON. 2014;19(4):895-9.
- Mørck TA, Loock KV, Poulsen MB, Siersma VD, Nielsen JKS, Hertel O, et al. Micronucleus frequency in Danish schoolchildren and their mothers from the DEMOCOPHES population. Mutagenesis 2016;31(1):1-8. [DOI:10.1093/mutage/gev054] [PMID]
- Souza AD, Devi R. Cytokinesis blocked micronucleus assay of peripheral lymphocytes revealing the genotoxic effect of formaldehyde exposure. Clin Anat. 2014;27(3):308-12. [DOI:10.1002/ca.22291] [PMID]
- Dave G, Parikh N, Patel N, joshi H. Evaluation of Micronucleus Frequencies in Exfoliated Buccal Cells of Potentially Malignant Disorder Subjects Using Feulgen Stain. Indian J Dent Adv. 2019; 11(3): 86-92. [DOI:10.5866/2019.11.10086]
- Sommer S, Buraczewska I, Kruszewski M. Micronucleus Assay: The State of Art, and Future Directions. Int J Mol Sci 2020; 21(4): 1534; [DOI:10.3390/ijms21041534] [PMID]
- Ceppi M, Biasotti B, Fenech M, Bonassi S. Human population studies with the exfoliated buccal micronucleus assay: statistical and epidemiological issues. Mutat Res. 2010;705(1): 11-9. [DOI:10.1016/j.mrrev.2009.11.001] [PMID]
- Shahsavari F, Mikaeli S, Ghorbanpour M. Micronucleus assay in the exfoliated cells of buccal mucosa of gasoline station workers in Tehran. J Can Res Ther. 2022;18:1030-5. [DOI:10.4103/jcrt.JCRT_875_20] [PMID]
- Hopf N, Bolognesi C, Danuser B, Wild P. Biological monitoring of workers exposed to carcinogens using the buccal micronucleus approach: A systematic review and meta-analysis. Mutat Res Rev Mutat Res. 2019;781:11-29. [DOI:10.1016/j.mrrev.2019.02.006] [PMID]
- Aglan M, N.Mansour Gh. Hair straightening products and the risk of occupational formaldehyde exposure in hairstylists. Drug Chemic Toxicol. 2020; 43(5): 488-95. [DOI:10.1080/01480545.2018.1508215] [PMID]
- Abdel Aziz MH, Metwally EA, Zaki EIA, A. Azzaz O, Hussein H.A. Is duration of exposure a determinant factor for genotoxicity and clinical manifestations induced by Formaldehyde? Ain Shams J Forensic Med Clin Toxicol. 2021;36(1): 31-48. [DOI:10.21608/ajfm.2021.135371]
- Costa S, Costa C, Madureira J, Valdiglesias V, Teixeira-Gomes A, de Pinho PG, et al. Occupational exposure to formaldehyde and early biomarks of cancer risk, immunotoxicity and susceptibility. Environ Res. 2019; 179:108740. [DOI:10.1016/j.envres.2019.108740] [PMID]
- Neelima P, Ravi S. Micronuclei assay in buccal smear of formalin exposed individuals. Indian J Basic Appl Med Res 2019;8 (4):343 -6.
- Souza AD, Devi R. Micronucleus assay on buccal cells: an indicator of DNA damage due to formaldehyde exposure in anatomy dissection labs. Int J Res Med Sci. 2014;2(1):71-4. [DOI:10.5455/2320-6012.ijrms20140214]
- Costa S, Brandao F, Coelho M, Costa C, Coelho P, Silva S, et al. Micronucleus frequencies in lymphocytes and buccal cells in formaldehyde exposed workers. WIT Transactions on Biomedicine Health. 2013;16,10:83-94. [DOI:10.2495/EHR130081]
- Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res. 1992;271(1):69-77. [DOI:10.1016/0165-1161(92)90033-I] [PMID]
- Costa S, Coelho P, Costa C, Silva S, Mayan O, Santos LS, et al. Genotoxic damage in pathology anatomy laboratory workers exposed to formaldehyde. Toxicology. 2008;252(1-3):40-8. [DOI:10.1016/j.tox.2008.07.056] [PMID]
- Ladeira C, Viegas S, Carolino E, Gomes MC, Brito M. The influence of genetic polymorphisms in XRCC3 and ADH5 genes on the frequency of genotoxicity biomarkers in workers exposed to formaldehyde. Environ Mol Mutagen. 2013;54(3):213-21. [DOI:10.1002/em.21755] [PMID]
- Ladiera C, Veigas S, Carolino E, Prista T, Gomes MC, Brito M. Genotoxic biomarkers in occupational exposure to formaldehyde. The case of histopathology laboratories. Mutat Res. 2011;721:15-20. [DOI:10.1016/j.mrgentox.2010.11.015] [PMID]
- Bouraoui S, Mougou S, Brahem A, Tabka F, Ben Khelifa H, Harrabi I, et al. A combination of micronucleus assay and fluorescence in situ hybridization analysis to evaluate the genotoxicity of formaldehyde. Arch Environ Contam Toxicol. 2013;64(2):337-44. [DOI:10.1007/s00244-012-9828-6] [PMID]
- Fenech M, Nersesyan A, Knasmueller S. A systematic review of the association between occupational exposure to formaldehyde and effects on chromosomal DNA damage measured using the cytokinesis-block micronucleus assay in lymphocytes. Mutat Res Rev Mutat Res. 2016;770(Pt A):46-57. [DOI:10.1016/j.mrrev.2016.04.005] [PMID]
- Peteffi GP, Antunes MV, Carrer C, Valandro ET, Santos S, Glaeser J, et al. Environmental and biological monitoring of occupational formaldehyde exposure resulting from the use of products for hair straightening. Environ Sci Pollut Res Int. 2016;23(1):908-17. [DOI:10.1007/s11356-015-5343-4] [PMID]
- Sivasankari Natarajan P, Subramanian S, Elayaperumal S. Formalin induced nuclear abnormalities in relation with duration of exposure: a study of formalin exposed anatomists, attenders and students. Int J Anat Res. 2019;7(2.3):6616-20. [DOI:10.16965/ijar.2019.188]
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