Hamid Galehdari; Fariborz Soheili; Ali Mohammad Foroughmand; Abdolreza Masjedizadeh
Volume 5, Issue 3 , June 2010, , Pages 116-120
Abstract
Objectives and Background: Mutation directed inactivation of the tumor suppressor gene p53 have been found incountries with high frequency for hepatocellular carcinomas (HCCs). Our goal in the present study was screening of the p53 gene in tumor tissues from HCC affected individuals in southwest ...
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Objectives and Background: Mutation directed inactivation of the tumor suppressor gene p53 have been found incountries with high frequency for hepatocellular carcinomas (HCCs). Our goal in the present study was screening of the p53 gene in tumor tissues from HCC affected individuals in southwest Iran for putative mutations in exons 7 and 8 that are known as hot spot regions. Materials & Methods: A total of 25 archival formalin fixed paraffin embedded samples prepared from 1997 to 2006 were collected from hospitals in southwest and northwest of Iran. We examined the codon 249 within the exon 7 using RFLP as well the full-length sequencing of exons 7 and 8. Results: The samples were diagnosed as HCC and classified in four groups, well differentiated (39%), moderately differentiated (54%), poorly differentiated (4.5%) and undifferentiated (2.5%). The hepatitis B virus (HBV) was detected in 16% (n=7) and 11% (n=5) of patient’s sera that were affected with liver cirrhosis. No patient was infected with hepatitis C virus. However, in one tumor sample a homozygote mutation was detected at codon 302. Conclusion: In contrast to the numerous reports, particularly from Asian countries, the selected HCC patients in this study show a very low mutation rate in the hot spot regions of the p53 gene. However, further mutation studies have to be conducted for the whole length of the p53 gene for more evaluation.
Hamid Galehdari; Azam Ahmadi Shadmehr; Mahmoud Kalantar; Alimohammad Foroughmand; Rasekh -
Volume 4, Issue 1 , January 2009, , Pages 26-31
Abstract
Background and Objectives: Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant cancer predisposition syndrome caused by germ-line mutations in DNA mismatch repair genes. Tumors arising as a result of these mutations display instability in a sequence area known as microsatellites. ...
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Background and Objectives: Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant cancer predisposition syndrome caused by germ-line mutations in DNA mismatch repair genes. Tumors arising as a result of these mutations display instability in a sequence area known as microsatellites. Studies have shown that some Bethesda markers (BAT25, BAT26) are more efficient than others in identifying Microsatellite Instability (MSI) in tumors of HNPCC patients. The aim of this study was toevaluate the possible benefits of two MSI markers BAT25 and BAT26 to identifying microsatellite instability in tumor tissues from HNPCC patients. Material & Methods: We used 49 cases gathered from north-east Iran. Microsatellite Instability analysis was performed using fluorescent-labeled primers. Statistical analysis was achieved using SPSS software. Results: 24/5% (12/49) and 34.7% (17/49) of the cases showed MSI in BAT25 and BAT26, respectively. None of tumor sample was MSI positive for both markers. Discussion: MSI frequency is considerably lower, compared to other findings. This might be due to the fact that environment and Race has great influence on MSI frequency.