Iranian Journal of Pathology

An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

Document Type : Original Research

Authors

R, Salehi 1
B. Tabanifar 2
E. Asgarani 3
M. Faghihi 4
T. Allame 5

1 Dept. of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

2 Dept. of Microbiology, School of Biology, Alzahra University, Tehran, Iran.

3 Dept. of Genetics, School of Biology, Alzahra University, Tehran, Iran.

4 Dept. of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

5 Dept. of Gynecology and Obstetrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract
  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation. Results: This method produced DNA suitable for amplification using primers specific for human SMN and β globin genes in 98.63% and 82.2% of samples respectively. We also detected human papillomavirus DNA in 58.33% of appropriate samples. Conclusion: This procedure provides simple and efficient method for recovery of amplifiable genomic and viral DNA from paraffin wax embedded tissues.  

Keywords

DNA
Paraffin Embedding
Polymerase chain reaction
Iranian Journal of Pathology
Volume 3, Issue 4 - Serial Number 4
Autumn 2008
Pages 173-178

  • Receive Date 29 September 2014

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