@article { author = {Salehi, R, and Tabanifar, B. and Asgarani, E. and Faghihi, M. and Allame, T.}, title = {An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA}, journal = {Iranian Journal of Pathology}, volume = {3}, number = {4}, pages = {173-178}, year = {2008}, publisher = {Farname Inc in collaboration with Iranian Society of Pathology}, issn = {1735-5303}, eissn = {2345-3656}, doi = {}, abstract = {  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation. Results: This method produced DNA suitable for amplification using primers specific for human SMN and β globin genes in 98.63% and 82.2% of samples respectively. We also detected human papillomavirus DNA in 58.33% of appropriate samples. Conclusion: This procedure provides simple and efficient method for recovery of amplifiable genomic and viral DNA from paraffin wax embedded tissues.  }, keywords = {DNA,Paraffin Embedding,Polymerase chain reaction}, url = {https://ijp.iranpath.org/article_9002.html}, eprint = {https://ijp.iranpath.org/article_9002_917d8daf6ef67bc61d30f62c5c3ea8bc.pdf} }