Iranian Journal of Pathology

Simultaneous Genetically Detection of Streptococcus pyogenes, Streptococcus pneumoniae and Haemophilus influenzae in Patients with Treatment-Resistant Respiratory Infection

Document Type : Original Research

Authors

Farzad Mohammadi Ebli 1
Zoheir Heshmatipour 2
Khadijeh Daneshjou 3
Seyed Davood Siadat 4

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

3 Department of Pediatrics, Imam Khomeini Hospital, School of Medicine, Tehran University of Medical Science, Tehran, Iran

4 Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran

https://doi.org/10.30699/ijp.2023.1991067.3075
Abstract
Background & Objective: Streptococcus pneumoniae, Haemophilus influenzae and Streptococcus pyogenes are among the most important causes of infection in human. Inventing rapid methods to identify these species can help in providing appropriate and effective treatment options. Therefore, the current study aimed to develop a multiplex touch-down PCR method to identify rapidly the aforementioned species patients' sputum samples, simultaneously.
Methods: A total of 50 sputum samples of patients with respiratory infections resistant to treatment were collected. After DNA extraction and primer design, the complete capsule (CAP) region II, capsular polysaccharide biosynthesis (cpsA) and the structural regulator of transcription (spy) genes were amplified for detecting H. influenzae, S. pneumoniae and S. pyogenes by multiplex touch-down PCR.
Results: Among 50 samples prepared from patients with different diseases, 27 samples were positive for amplified genes. The frequency of presence of pathogens in the collected samples included 14% H. influenzae, 20% S. pneumoniae and 20% S. pyogenes. Also, in some patients, the simultaneous presence of two or three pathogens were observed.
Conclusion: In general, it can be concluded that the PCR touchdown method developed in the present study is an effective and fast method for the simultaneous identification of H. influenzae, S. pneumoniae and S. pyogenes pathogens in clinical samples of patients.

Keywords

Gene
Multiplex PCR
Pathogen
Sputum

Subjects

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Iranian Journal of Pathology
Volume 18, Issue 3
Summer 2023
Pages 356-362

  • Receive Date 19 January 2023
  • Revise Date 06 March 2023
  • Accept Date 15 July 2023

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