Document Type : Original Research


1 Dept. of Biology, Payame Noor University, Iran

2 Nano Structured Coatings Institute, Yazd Payame Noor University, Yazd, Iran



Background & Objectives: Direct addition of antimicrobial materials to food during food processing is an effective method for controlling microbial contaminants of food and extending the shelf-life of food products. Objective of this research was to study the antimicrobial effect of zinc oxide (ZnO) nanoparticle and potential applications of ZnO nanoparticles in terms of controling two food-borne pathogens in milk. Methods: Toxicity of different concentration (0, 0.5, 2, 5, and 10 mM) of ZnO nanoparticles on Listeria monocytogenesand Bacillus cereuswas studied in culture media and milk. Results: Among the mentiond concentrations, treatment of 10 mM of ZnO nanoparticle was the most effective one for L. monocytogenesand B. cereus inhibition, which completely inhibited the  growth ofL. monocytogenesand B. cereusin 24h. These data revealed concentration-dependency of the antibacterial activity of ZnO. Therefore, 5 mM and 10 mM ZnO were selected for further studies,  which  were  performed  in  milk,  since  they  demonstrated  significant  growth  inhibition. ZnO NPs were more capable in terms of reducing the initial growth counts of all the above-stated strains in milk. Conclusion: ZnO nanoparticles had an antimicrobial activity against L. monocytogenes and B. cereusin milk and the media. This work was a preliminary study that provided a starting point for determining whether the use of ZnO nanoparticles had the potential for being applied in food preservation or not.


  1. Okouchi S, Murata R, Sugita H, Moriyoshi Y, Maeda N. Calorimetric evaluation of the antimicrobial activities of calcined dolomite. J Antibact Antifungal Agents 1995; 26(3): 109–14.
  2. Wilczynski M. Anti-microbial porcelain enamels. Ceram Eng Sci Proc 2000; 21(5): 81–3.
  3. Xie Y, He Y, Irwin PL, Jin T, Shi X. Antibacterial activity and mechanism of action of zinc oxide nanoparticles against  Campylobacter jejuni. Appl Environ Microb 2011; 77(7): 2325–31.
  4. Huang Z, Maness PC, Blakee DM, Wolfrum EJ, Smoliski SL, Jacoby WA. Bacterial mode of titanium dioxide photocatalysis. J Photochem Photobiol A: Chem 2000; 130(2-3): 163–70.
  5. Shirashi F, Toyoda K, Fukinbara S. Photolytic and photocatalytic treatment of an aqueous solution containing microbial cells and organic compounds in an annular- flowre actor. Chem Eng Sci 1999; 54(10): 1547–52.
  6. Kourai H. Immobilized microbiocide. J Antibact Antifungal Agents 1993; 21(6): 331–7.
  7. Wang YL, Wan YZ, Dong XH, Cheng GX, Tao HM, Wen T et al.Preparation and characterization of  antibacter  ial  viscose-based  activated  carbon  fiber supporting silver. Carbon 1995; 36(11): 1567–71.
  8. Padmavathy N, Vijayaraghavan R. Enhanced bioactivity of ZnO nanoparticles—an antimicrobial study. Sci Technol Adv Mater 2008; 9(3): 1-7.
  9. Nicole J, Binata R, Koodali T, Ranjit C. Antibacterial activity of ZnO nanoparticle suspensions on abroad spectrum of microorganisms. FEMS Microbiol Lett 2008; 279(1): 71–6.
  10. Tiller JC, Liao CJ, Lewis K, Klibanov AM. Designing surfaces that kill bacteria on contact. Proc Natl Acad Sci USA 2001; 98(11): 5981–5.
  11. Espitia PJP, Soares NdFF, Coimbra JSdR, de Andrade NJ, Cruz RS, Medeiros EAA. Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications. Food and Bioprocess Technology. 2012;5(5):1447-64.
  12. Emamifar A, Kadivar M, Shahedi M, SoleimanianZad S. Effect of nanocomposite packaging containing Ag and ZnO on inactivation of Lactobacillus plantarum in orange juice. Food Control 2011;22(3-4):408-13.
  13. Lopes DE, Romana D, Brown KH, Guinard JX. Sensory trial to assess the acceptability of zinc for tificants added to iron-fortified wheat products. J Food Sci 2002; 67(1): 461–5.
  14. Saldamli I, Kokshel H, Ozboy O, Ozalp I, Kilic I. Zinc-supplemented bread and its utilization in zinc deficiency. Cereal Chem 1996; 73(4): 424–7.
  15. Mirhosseini M, Emtiazi G. Optimisation of Enterocin A Production on a Whey-Based Substrate. World Appl Sci J 2011; 14(10): 1493-9.
  16. Jin T, Sun D, Su JY, Zhang H, Sue HJ. Antimicrobial Efficacy of Zinc Oxide Quantum Dots against Listeria monocytogenes, Salmonella enteritidisand Escherichia coliO157:H7. J Food Sci 2009; 74(1): 46-52.
  17. Weiss J, Takhistov P, Clements DJ. Functional materials in food nanotechnology. J Food Sci 2006; 71(9): 107–16.
  18. Moraru CI, Panchapakesan CP, Huang Q, Takhistove P, Liu S, Kokini JL. Nanotechnology: a new frontier in food science. Food Technol 2003; 57(12): 24–9.
  19. Sawai J, Yoshikawa T. Quantitative evaluation of antifungal activity of metallic oxide powders (MgO, CaO and ZnO) by an indirect conductimetric assay. J Appl Microbiol 2004; 96(4): 803–9.
  20. Sawai J. Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. J Microbiol Methods 2003; 54(2):177–82.
  21. Sunada K, Kikuchi Y, Hashimoto K, Fujishima K. Bactericidal and detoxification effects of TiO2 thin film photocatalysts. Environ Sci Technol 1998; 32(5): 726–8.
  22. Sunada K, Watanabe T, Hashimoto K. Disinfection of surfaces by photocataly ticoxidation with titanium dioxide and UVA light. J Photochem Photobiol A: Chem 2003; 156(1-3): 227–33.
  23. Brayner R, Ferrari-Iliou R, Brivois N, Djediat S, Benedetti MF, Fievet F. Toxicological impact studies based on Escherichia coli bacteria  in  ultrafine  ZnO nanoparticles colloidal medium. Nano Letters 2006; 6(4): 866–70.
  24. Mirhosseini M, Firouzabadi FB. Antibacterial activity of Zinc oxide nanoparticle suspensions on foodborne pathogens. Int J Dairy Technol 2013; 66(2): 291-5.
  25. Huang Z, Zheng X, Yan D, Yin G, Liao X, Kang Y. Toxicological effect of ZnO nanoparticles based on bacteria. Langmuir 2008; 24(8): 4140–4.
  26. Liu Y, He L, Mustapha A, Li H, Hu ZQ, Lin M. Antibacterial activities of zinc oxide nanopartic les against Escherichia coli O157:H7. J Appl Microbiol 2009; 107(4): 1193–201.