Iranian Journal of Pathology

Use Of Razi Bovine Kidney Cell Line For Proliferation Of Pneumocystis Carinii

Document Type : Original Research

Authors

Massoud Hajia 1
Abbas Mahmoodzadeh 2
Hassan Morovati 3

1 Research Center of Health Reference Laboratory, Tehran, Iran.

2 Dept. of Microbiology and Research Center of Molecular Biology, Baqyatallah University of Medical Sciences, Tehran, Iran.

3 Razi Vaccine and Serum Research Institute, Karaj, Iran.

Abstract
  Background and Objective: Pneumocystis pneumonia (PCP) has been historically the most prevalent opportunisticinfection in patients infected with the human immunodeficiencyvirus. Culture of the organism has not been faced with suitable success in artificial media, while various results have been reported for cell culture media. The aim of this study was proliferation of Pneumocystis carinii on the Razi Bovine Kidney (RBK) cell line and to compare growth rate with ‘Vero’ and ‘MRC-5’ cell lines. Materials and Methods: We used 6 rats (Sprague-Dawley) provided from Razi Institute to infect with Pneumocystis carinii after suppressing the immune system with methylprednisolone acetate (40 mg/kg). Methylprednisolone acetate was used subcutaneously once a week for 8 weeks. Samples were homogenized after separation of the lung tissue. Microscopic examination was applied for prepared smears to confirm the presence of Pneumocystis carinii. Purified trophozoites were then inoculated into the cell line flasks. Growth rate was estimated by counting the trophozoite in each day. Results: Number of cultivated organisms was increased after 5 days incubation in all applied cell lines. Growth rate of Vero, MRC-5 and RBK were 3, 3, and 3.75 times more respectively in comparison with number of the calculated cells in first day. Hence the difference between RBK and two other cell lines was significant (p = 0.023). Conclusion: RBK cell line is suitable to proliferate Pneumocystis carinii.  

Keywords

Pneumocystis carinii
Vero cells
MRC-5 cell
Razi bovine kidney cell
Iranian Journal of Pathology
Volume 3, Issue 4 - Serial Number 4
Autumn 2008
Pages 197-202

  • Receive Date 29 September 2014

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