Document Type : Original Research


1 Dept. of Clinical Research, Pasteur Institute of Iran, Tehran, Iran

2 Dept. of Pathology, Cancer Institute of Iran, Tehran, Iran

3 Dept. of Virology, Tehran University of Medical Sciences, Tehran, Iran

4 Dept. of Gastroenterology, Tehran University of Medical Sciences, Tehran, Iran

5 Dept. of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Background and Objective: Human papillomavirus (HPV) is one of the possible etiologic factors  in development of esophageal squamous cell carcinoma (ESCC). In this study we aimed to study the  role of HPV in ESCC.
Patients and Methods:In this study, 140 cases of ESCC were analyzed for the HPV DNA by  polymerase chain reaction (PCR) using GP5+/GP6+ primers for L1 open reading frame (ORF) to  amplify a 150-bp segment of HPV L1 ORF. This region was subsequently sequenced to identify the  type of HPV.
Results:A total of 140 patients enrolled in our study. In this respect, 50.7% of them were females and 49.3% were males, aged between 20 and 81 years old. In addition, 33 tumor specimens (23.6%) and 12 (8.6%) non-involved tumor margins were HPV positive. In HPV positive tumor cases, 36% were also positive in tumor margins. The HPV positive cases were 21.7% males and 25.3% females. There was no correlation between the presence and types of HPV with patients’ sex and age. The frequency of HPV subtypes in tumoral regions were as follow: HPV-16: 60.6%, HPV-18: 30.3%, HPV-33: 6.1%, and HPV-31: 3 %. We found only HPV-16 in tumor margins.
Conclusion:Our results support a causal association between HPV infection and ESCC which is consistent with HPV studies conducted in other high-risk areas.