Iranian Journal of Pathology

Searching the Human Herpes 6, 7 (PCR) In CSF of Children Admitted In Pediatric Ward of Rasoul Hospital, Tehran, Iran

Document Type : Original Research

Authors

Farideh Ebrahimi Taj 1
Samileh Noorbakhsh 1
Hamid Reza Monavari 2
Azardokht Tabatabaei 3
Masomeh Bakhshyeh 3

1 Dept. of Infectious Disease, Research Center of Pediatric Infectious Diseases, Tehran University of Medical Sciences, Tehran, Iran

2 Dept. of Virology, Research Center of Pediatric Infectious Diseases, Tehran University of Medical Sciences, Tehran, Iran

3 Dept. of Microbiology, Research Center of Pediatric Infectious Diseases, Tehran University of Medical Sciences

Abstract
Background and Objectives: Meningoencephalitis in Iranian children is frequent, but encephalitis is rare .The frequency of HHV-6 and HHV-7 in central nervous system diseases of our children is unclear. The aim of this study was searching the DNA-s of HHV-6 & HHV-7 in CSF samples of children with meningoencephalitis 
Materials and Methods: A cross sectional study (2007-2009) was done in Pediatric Ward in Rasoul Hospital, Tehran Iran. Totally, 150 CSF samples obtained from children (1-180 months) with meningoencephalitis. Conventional and BACTEC Ped Plus medium, latex agglutination tests were used for ruling out the bacterial causes. We searched the DNA-s of HHV-6 & HHV-6 quantitatively by Real time - PCR in 150 CSF samples obtained from children with meningoencephalitis.
Results: 60.7 % of  cases were male. Fever was reported (>38.5) in 74%; irritability in 70%; Convulsion was seen in 53% of cases. Herpes virus was detected in 12% (18/150) of cases. Both HHV-6 & HHV-7 were found in 6% of all cases. HHV-6 DNA was detected in 4.7% (6) and HHV-7 DNA was detected in 2 cases (1.4%) without any correlation to age, sex and clinical signs. HHV-6 was slightly more frequent than HHV-7.
Conclusion: Our data indicate that herpes viruses are not uncommon causes in children with meningoencephalitis. The incidence was lower than other references.
 

Keywords

Cerebrospinal Fluids
Herpes viruses
PCR
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Iranian Journal of Pathology
Volume 7, Issue 2 - Serial Number 2
Spring 2012
Pages 107-111

  • Receive Date 26 December 2010
  • Accept Date 20 June 2011

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