Document Type : Original Research

Authors

1 Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran

2 Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran

Abstract

 
Background and Objectives: Biological scaffold resources composed of extracellular matrix (ECM) have been shown to make easy the practical remodeling of various tissues in both animal and human studies. The goal of current study was to make sheet form of ECM from sheep’s urinary bladder.
Methods: ECM was extracted from Sheep’s urinary bladder according to standard method. Scanning electron microscopy (SEM) was applied in order to analyze the ultrastructure of the extracted matrix.
Results: Matrix was formed by irregular and fiber like particles.
Conclusion: Sheep’s urinary bladder matrix may be used as an accessible and suitable source of ECM extraction.
 

Keywords

  1. Badylak SF, Freytes DO, Gilbert TW. Extracellular matrix as a biological scaffold material: Structure and function. Acta Biomaterialia 2009; 5: 1–13.
  2. Badylak SF. The extracellular matrix as a biologic scaffold material. Biomaterials 2007; 28:  3587–93.
  3. Brown B, Lindberg K, Reing J, Stolz DB, Badylak SF. The basement membrane component of biologic scaffolds derived from extracellular matrix. Tissue Eng 2006; 12(3): 519-26.
  4. Brennan EP, Reing J, Chew D, Myers-Irvin JM, Young EJ, Badylak SF. Antibacterial activity within degradation products of biological scaffolds composed of extracellular matrix. Tissue Eng 2006; 12(10): 2949-55.
  5. Kimmel H, Rahn M, Gilbert TW. The clinical effectiveness in wound healing with extracellular matrix derived from porcine urinary bladder matrix: a case series on severe chronic wounds. The Journal of the American College of Certified Wound Specialists 2010; 2(3): 55–9.
  6. Gilbert TW, Nieponice A, Spievack AR, Holcomb J, Gilbert S, Badylak SF. Repair of thoracic wall with an extracellular matrix scaffold in a canine model. J Surgical Res 2008; 147(1): 61-7.
  7. Gilbert TW, Stolz DB, Biancaniello F, Simmons-Byrdd A, Badylak SF. Production and characterization of ECM powder: implications for tissue engineering applications. Biomaterials 2005; 26: 1431–5.
  8. Badylak SF, Lantz GC, Coffey A, Geddes LA. Small intestinal submucosa as a large diameter vascular graft in the dog. J Surg Res 1989; 47(1):74–80.
  9. Chen F, Yoo JJ, Atala A. Acellular collagen matrix as a possible ‘‘off the shelf” biomaterial for urethral repair. Urology 1999; 54(3): 407–10.
  10. Lin P, Chan WC, Badylak SF, Bhatia SN. Assessing porcine liver derived biomatrix for hepatic tissue engineering. Tissue Eng 2004; 10(7–8):1046–53.
  11. Badylak SF, Tullius R, Kokini K, Shelbourne KD, Klootwyk T, Voytik SL, et al. The use of xenogeneic small intestinal submucosa as a biomaterial for Achilles tendon repair in a dog model. J Biomed Mater Res 1995; 29(8): 977–85.
  12. Brown B, Lindberg K, Reing J, Stolz DB, Badylak SF. The basement membrane component of biologic scaffolds derived from extracellular matrix. Tissue Eng 2006; 12(3): 519–26.
  13. McDevitt CA, Wildey GM, Cutrone RM. Transforming growth factor-beta1 in a sterilized tissue derived from the pig small intestine submucosa. J Biomed Mater Res A 2003; 67(2): 637–40.
  14. Voytik-Harbin SL, Brightman AO, Kraine MR, Waisner B, Badylak SF. Identification of extractable growth factors from small intestinal submucosa. J Cell Biochem 1997; 67(4): 478–91.
  15. Hodde JP, Ernst DM, Hiles MC. An investigation of the long-term bioactivity of endogenous growth factor in OASIS Wound Matrix. J Wound Care 2005; 14(1): 23–5.
  16. Hodde JP, Record RD, Liang HA, Badylak SF. Vascular endothelial growth factor in porcine-derived extracellular matrix. Endothelium 2001; 8(1): 11–24.