Iranian Journal of Pathology

Prevalence of algD, pslD, and pelF Genes Involved in Biofilm Formation in Clinical MDR Pseudomonas aeruginosa Strains

Document Type : Original Research

Authors

Zahra Haghighatian 1
Negar Shirani 2
Gholamreza Goudarzi 3
Pegah Shakib 4
Fatemeh Jahani 5
Sodabeh Zare 6

1 Department of Pathology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

2 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran

3 Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

5 Department of Biology, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran

6 Department of Biostatistics and Epidemiology, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran

https://doi.org/10.30699/ijp.2025.2057524.3439
Abstract
Background & Objective: The purpose of this research was to determine the frequency of algD, pslD, and pelF genes in biofilm formation among MDR and non-MDR clinical strains of Pseudomonas aeruginosa in Khorramabad, Iran (2024).
Methods: This cross-sectional study included all Pseudomonas aeruginosa isolates collected from various clinical samples in Khorramabad teaching hospitals in 2024. After confirming the isolates and determining their antibiotic resistance patterns using the disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, algD, pelF, and pslD genes were detected by PCR.
Results: The highest sensitivity was observed to imipenem (75%) and meropenem (71.3%), while the greatest resistance was recorded against ciprofloxacin, ceftazidime, and tobramycin 45 (56.25%). The frequencies of the algD, pelF, and pslD genes were 88.8, 76.3, and 96.3%, respectively. A significant association was found between the PelF and algD genes with multidrug resistance (MDR) (P<0.05).
Conclusion: The presence of multi-drug resistance (MDR) in this study indicates the need for serious measures to control infections caused by this bacterium. Further research is recommended to explore the contribution of biofilm-associated genes to the  development of multi-drug resistance (MDR).

Keywords

Pseudomonas aeruginosa
Biofilm
Multidrug resistance (MDR)
Exopolysaccharides

Subjects

  1. Vetrivel A, Ramasamy M, Vetrivel P, Natchimuthu S, Arunachalam S, Kim G, et al. Pseudomonas aeruginosa Biofilm Formation and Its Control. Biologics. 2021, 1, 312-336. [DOI:10.3390/biologics1030019]
  2. Diggle SP, Whiteley M. Microbe Profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat. Microbiology. 2020;166(1):30-3. [DOI:10.1099/mic.0.000860] [PMID] [PMCID]
  3. Horcajada JP, Montero M, Oliver A, Sorlí L, Luque S, Gómez-Zorrilla S, et al. Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections. Clin Microbiol Rev. 2019;32(4):10.1128. [DOI:10.1128/CMR.00031-19] [PMID] [PMCID]
  4. Kunz Coyne AJ, El Ghali A, Holger D, Rebold N, Rybak MJ. Therapeutic strategies for emerging multidrug-resistant Pseudomonas aeruginosa. Infectious diseases and therapy. 2022 Apr;11(2):661-82. [DOI:10.1007/s40121-022-00591-2] [PMID] [PMCID]
  5. Strateva T, Mitov I. Contribution of an arsenal of virulence factors to pathogenesis of Pseudomonas aeruginosa infections. Ann microbiol .2011;61:717-32. [DOI:10.1007/s13213-011-0273-y]
  6. Huber P, Basso P, Reboud E, Attrée I. Pseudomonas aeruginosa renews its virulence factors. Environ Microbiol Rep. 2016;8(5):564-71. [DOI:10.1111/1758-2229.12443] [PMID]
  7. Le Berre R, Nguyen S, Nowak E, Kipnis E, Pierre M, Quenee L, Ader F, Lancel S, Courcol R, Guery BP, Faure K. Relative contribution of three main virulence factors in Pseudomonas aeruginosa pneumonia. Critic Care Med. 2011;39(9):2113-20. [DOI:10.1097/CCM.0b013e31821e899f] [PMID]
  8. Nadal Jimenez P, Koch G, Thompson JA, Xavier KB, Cool RH, Quax WJ. The multiple signaling systems regulating virulence in Pseudomonas aeruginosa. Microbiol Mol Biol Rev . 2012;76(1):46-65. [DOI:10.1128/MMBR.05007-11] [PMID] [PMCID]
  9. Ciofu O, Tolker-Nielsen T. Tolerance and resistance of Pseudomonas aeruginosa biofilms to antimicrobial agents-how P. aeruginosa can escape antibiotics. Front Microbiol. 2019;10:913. [DOI:10.3389/fmicb.2019.00913] [PMID] [PMCID]
  10. Chung J, Eisha S, Park S, Morris AJ, Martin I. How three self-secreted biofilm exopolysaccharides of Pseudomonas aeruginosa, Psl, Pel, and alginate, can each be exploited for antibiotic adjuvant effects in cystic fibrosis lung infection. Int J Mol Sci . 2023;24(10):8709. [DOI:10.3390/ijms24108709] [PMID] [PMCID]
  11. Tian L, Xu S, Hutchins WC, Yang C-H, Li J. Impact of the exopolysaccharides Pel and Psl on the initial adhesion of Pseudomonas aeruginosa to sand. Biofouling. 2014;30(2):213-22. [DOI:10.1080/08927014.2013.857405] [PMID]
  12. Gheorghita AA. Structural Insights Into Modification and Export of the Pseudomonas aeruginosa Alginate Exopolysaccharide: University of Toronto (Canada); 2023. [DOI:10.1038/s41467-022-35131-6]
  13. Dharshini RS, Manickam R, Curtis WR, Rathinasabapathi P, Ramya M. Genome analysis of alginate synthesizing Pseudomonas aeruginosa strain SW1 isolated from degraded seaweeds. Antonie Van Leeuw .2021;114:2205-17. [DOI:10.1007/s10482-021-01673-w] [PMID]
  14. Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. CLSI methods development and standardization working group best practices for evaluation of antimicrobial susceptibility tests. J Clin Microbiol .2018;56(4):10.1128. [DOI:10.1128/JCM.01934-17] [PMID] [PMCID]
  15. As S. CLSI-based antibiogram profile and the detection of MDR and XDR strains of Acinetobacter baumannii isolated from urine samples. Med J Islam Repub Iran .2019;33:3. [DOI: 10.34171/mjiri.33.3] [PMID] [PMCID]
  16. Heydari S, Eftekhar F. Biofilm formation and β-lactamase production in burn isolates of Pseudomonas aeruginosa. Jundishapur journal of microbiology. 2015;8(3):e15514. [DOI:10.5812/jjm.15514] [PMID] [PMCID]
  17. Banar M, Emaneini M, Satarzadeh M, Abdellahi N, Beigverdi R, Leeuwen WBv, et al. Evaluation of mannosidase and trypsin enzymes effects on biofilm production of Pseudomonas aeruginosa isolated from burn wound infections. PloS one. 2016; 11(10): e0164622. [DOI:10.1371/journal.pone.0164622] [PMID] [PMCID]
  18. Khan M, Willcox MD, Rice SA, Sharma S, Stapleton F. Development of antibiotic resistance in the ocular Pseudomonas aeruginosa clone ST308 over twenty years. Experiment Eye Res .2021;205:108504. [DOI:10.1016/j.exer.2021.108504] [PMID]
  19. Sebola D, Eliasi UL, Oguttu JW, Qekwana D. Antimicrobial resistance patterns of Pseudomonas aeruginosa isolated from canine clinical cases at a veterinary academic hospital in South Africa. J South Afric Veteri Associat .2020;91(1):1-6. [DOI:10.4102/jsava.v91i0.2052] [PMID] [PMCID]
  20. Vaez H, Salehi-Abargouei A, Ghalehnoo ZR, Khademi F. Multidrug resistant Pseudomonas aeruginosa in Iran: A systematic review and metaanalysis. J Global Infect Dis. 2018;10(4):212-7. [DOI:10.4103/jgid.jgid_113_17] [PMID] []
  21. Ghorashi Z, Nezami N, Ghotaslou R, Ghorashi S. Pattern of Pseudomonas aeruginosa drug resistance in Tabriz Children Hospital. PJBS .2010;13(8):400-4. [DOI:10.3923/pjbs.2010.400.404] [PMID]
  22. Gebreyohannes G, Nyerere A, Bii C, Sbhatu DB. Challenges of intervention, treatment, and antibiotic resistance of biofilm-forming microorganisms. Heliyon .2019;5(8). [DOI:10.1016/j.heliyon.2019.e02192] [PMID] [PMCID]
  23. Almzil NRH, Yazgan YK, Al Marjani M. Evaluation Of Biofilm Formation In Pseudomonas Aeruginosa Isolated From Clinical Samples And The Presence Of Biofilm-Related Genes (pelA, pslD AND algD). Europ J Mol Clin Med. 2022;9(7):3603-15. [DOI: 10.1186/s13104-020-4890-z] [PMID] [PMCID]
  24. Rajabi H, Salimizand H, Khodabandehloo M, Fayyazi A, Ramazanzadeh R. Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 genes involved in biofilm formation in clinical Pseudomonas aeruginosa strains. BioMed Res Int .2022;2022(1):1716087. [DOI:10.1155/2022/1716087] [PMID] [PMCID]
  25. Ghadaksaz A, Fooladi AAI, Hosseini HM, Amin M. The prevalence of some Pseudomonas virulence genes related to biofilm formation and alginate production among clinical isolates. J Appl Biomed. 2015;13(1):61-8. [DOI:10.1016/j.jab.2014.05.002]
  26. Colvin KM, Gordon VD, Murakami K, Borlee BR, Wozniak DJ, Wong GC, et al. The pel polysaccharide can serve a structural and protective role in the biofilm matrix of Pseudomonas aeruginosa. PLoS pathog .2011;7(1):e1001264. [DOI:10.1371/journal.ppat.1001264] [PMID] [PMCID]
Iranian Journal of Pathology
Volume 20, Issue 4
Summer 2025
Pages 380-385

  • Receive Date 09 April 2025
  • Revise Date 23 May 2025
  • Accept Date 17 July 2025

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