Iranian Journal of Pathology

Evaluation of HOTAIRM1, miR-196b, and HOXA9 as Oncogenic Markers in Patients with Acute Myeloblastic Leukemia

Document Type : Original Research

Authors

Fahimeh Norouzi 1
Mehdi Allahbakhshian 1
Nader Vazifeshiran 1
Zahra Hasanpoor 1
Mohsen Hamidpour 2

1 Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 HSC Research Centre, Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

https://doi.org/10.30699/ijp.2025.2030358.3309
Abstract
Background & Objective: miR-196b, HOXA9, GFI1, and PIM1 are key factors involved in cellular signaling pathways that contribute to the pathogenesis of malignancies, including acute myeloblastic leukemia (AML). Given their critical roles in AML progression, the present study aimed to investigate the gene expression levels of HOTAIRM1, miR-196b, HOXA9, GFI1, and PIM1 in AML patients compared to healthy controls.
Methods: A total of 30 AML patients and 10 healthy volunteers were enrolled in this study. Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll-Paque density gradient centrifugation. Gene expression levels of HOTAIRM1, miR-196b, HOXA9, GFI1, and PIM1 were assessed using real-time quantitative PCR (RQ-PCR). Statistical analyses were performed using Student’s t-test, one-way ANOVA, and Pearson correlation tests.
Results: The expression levels of HOTAIRM1, miR-196b, HOXA9, and GFI1 were significantly elevated in AML patients compared to healthy controls. Furthermore, t-test analysis revealed that the expressions of HOTAIRM1, HOXA9, and GFI1 significantly differed between AML-M3 and non-M3 AML subtypes.
Conclusion: These findings suggest that the investigated markers, particularly HOTAIRM1, HOXA9, and GFI1, may serve as potential clinical biomarkers for monitoring AML progression and could be valuable targets for early detection or therapeutic intervention.

Keywords

Lung cancer
Adenocarcinoma
Squamous cell carcinoma
TTF-1
Napsin A
p40
p63

Subjects

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Iranian Journal of Pathology
Volume 20, Issue 3
Summer 2025
Pages 307-315

  • Receive Date 06 January 2025
  • Revise Date 09 February 2025
  • Accept Date 28 February 2025

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