Iranian Journal of Pathology

The Association Between RACK1 Gene Polymorphisms rs1279736 and rs3756585 in Uterine Cervical Cancer with the Risk Factor of HPV: A Focus on Squamous Cell Carcinoma (SCC)

Document Type : Original Research

Authors

Maryam Fekri Soofi Abadi 1
Fatemeh Hashemi 2
Saeedeh Shojaeepour 3
Sahar Amirpour-Rostami 4
Mohammad Reza Zangouey 1
Sara Pourshaikhali 1
Fatemeh Pouya 5
Shahriar Dabiri 1

1 Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran

2 Department of Biochemistry & Molecular Biology, Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

4 Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

5 Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

https://doi.org/10.30699/ijp.2024.2019194.3236
Abstract
Background & Objective: Cervical cancer is the second most common malignancy among women worldwide. The Receptor for Activated C Kinase 1 (RACK1) has a key role in regulating the pathways of cell signaling, angiogenesis, cell migration, invasion, and metastasis. This study investigated the association of polymorphisms rs1279736 and rs3756585 with cervical cancer.
Methods:  In this study, 100 LB pap-smear and paraffin block samples of cervical squamous carcinoma associated with Human papillomavirus (HPV) were selected along with 100 LB smear cytology samples from healthy women with normal pap smears, serving as the control group. Genomic DNA was extracted from the samples, and the polymorphisms rs1279736 and rs3756585 in the RACK1 genes were examined using the RFLP-PCR and ARMS-PCR methods, respectively.
Results: The rs1279736 polymorphism shows that the chance of cervical cancer in individuals with CA and AA genotypes is 3.772 and 1.852 times that of CC genotypes, respectively. This suggests that the A allele is involved in developing cervical cancer (P=0.005; OR=2.113). The rs3756585 polymorphism indicates that the chance of cervical cancer in TG and GG genotypes is 0.373 and 4.235 times that of TT genotypes, respectively. This also points towards the involvement of the G allele in the development of cervical cancer (P=0.032; OR=1.800). Also, there was no significant relationship between the genotype of polymorphisms, age, and subtype of papillomavirus 16 and 18 in patients.
Conclusion: The present study was conducted for the first time. Our results showed that two polymorphisms are significantly associated with cervical cancer.

Highlights

  • RACK1 is a candidate gene associated with uterine cervical malignancies.
  • All cases studied showed invasive squamous cell carcinoma of the cervix with adjacent in-situ carcinoma and koilocytosis.
  • Genetic polymorphisms of rs1279736 and rs3756585 in RACK1 gene can determine the presence of cervical cancer due to papillomavirus.

Keywords

Cervical Cancer
HPV
Genetic Polymorphisms
RACK1

Subjects

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Iranian Journal of Pathology
Volume 20, Issue 1
Winter 2025
Pages 24-32

  • Receive Date 31 January 2024
  • Revise Date 31 July 2024
  • Accept Date 25 December 2024

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