Iranian Journal of Pathology

Detection of icaABCD Genes and Biofilm Formation in Clinical Isolates of Methicillin Resistant Staphylococcus aureus

Document Type : Original Research

Authors

Mohsen Mirzaee 1
Shahin Najar Peerayeh 2
Abdol-Majid Ghasemian 2

1 Dept. of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

2 Dept. of Bacteriology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran

Abstract
Background & Objectives:  Methicillin resistance Staphylococcus aureus (MRSA) is one of the most important pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. The aims of this study were to determine presence oficaABCD genes and relation to the biofilm formation in of MRSA isolates. Methods: Of the 63 MRSA clinical isolates collected from selected hospitals in Tehran, Iran,quantitative biofilm formation was determined by microtiter tissue culture plates (MtP). All MRSA isolates were examined for determination the icaABCD genes by using PCR method. Results: twenty nine (46%) of the isolates were strong produced biofilm on Mtp. All of the MRSA isolates carried icaD and icaC genes, whereas, the prevalence of icaA and icaB was 60.3% and 51% respectively. Conclusions: S. aureus clinical isolates have different capacity to production biofilm and adhesion. This may be caused by a different in the expression of biofilm genes and hetrogenicity in genetic origins.

Keywords

Staphylococcus aureus
biofilm
icaABCD
MRSA
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Iranian Journal of Pathology
Volume 9, Issue 4 - Serial Number 4
Autumn 2014
Pages 257-262

  • Receive Date 26 August 2013
  • Accept Date 24 June 2014

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