Document Type : Original Research


1 Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore, India

2 Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore


Background & Objective: The common epithelial ovarian tumors are classified into serous, mucinous, clear cell, endometrioid, the Brenner, mixed, and undifferentiated types. Cytoskeleton intermediate filament composition of ovarian tissues indicates that the cytokeratin and vimentin are observed in ovarian surface epithelium along with the common ovarian epithelial tumors. The current study aimed at investigating the cytokeratin and vimentin expression in epithelial ovarian tumors to establish a diagnostic relevance.
Methods: Sixty-six common epithelial ovarian tumors were studied using anti-cytokeratins (Monoclonal Mouse Anti-Human Cytokeratin Clones AE1/AE3; DAKO, Denmark,) and anti-vimentin (Monoclonal Mouse Anti-Vimentin, Clone V9; DAKO, Denmark,) to ascertain the intermediate filament profiles in formalin-fixed and paraffin-embedded surgical pathology materials.
Results: All ovarian epithelial tumors expressed cytokeratin in a uniform fashion. Vimentin was coexpressed with high intensity in 62.5% of serous carcinomas, mild intensity in 25% of mucinous adenocarcinoma, and moderate intensity in single case of endometrioid adenocarcinoma. Vimentin decoration in mucinous carcinoma had a focal involvement, whereas malignant endometrioid and serous decoration tended to involve larger areas. There was a significantly increased expression of vimentin in serous cystadenoma and serous carcinoma, compared with their mucinous counterparts. Also, vimentin expression and histologic grade of serous tumors showed a positive correlation. No association was found between vimentin expression and degree of differentiation in mucinous, endometrioid, and Brenner tumors.
Conclusion: The current investigation emphasized the efficiency of immunohistochemistry (IHC) typing as a tool for a more precise characterization of the origin and differentiation of human neoplasms.


  1. Rein BJD, Gupta S, Dada R, Safi J, Michener C, Agarwal A. Potential Markers for Detection and Monitoring of Ovarian Cancer. Journal of Oncology. 2011;2011
  2. Riccardi E, Grieco V, Verganti S, Finazzi M. Immunohistochemical diagnosis of canine ovarian epithelial and granulosa cell tumors. J Vet Diagn Invest.2007;19(4):431–5
  3.  Herrmann H, Aebi U. Intermediate filaments and their associates: multi-talented structural elements specifying cytoarchitecture and cytodynamics. Curr Opin Cell Biol. 2000;12(1):79-90.
  4. Kaku T, Ogawa S, Kawano Y, Ohishi Y, Kobayashi H, Hirakawa T, et al. Histological classification of ovarian cancer.Med Electron Microsc.2003;36(1):9-17.
  5. Bahrami A, Truong LD, Ro JY. Undifferentiated tumor: true identity by immunohistochemistry.Arch Pathol Lab Med.2008;132:326-48.
  6. Loffler S, Horn LC, Weber W, Spanel-Borowski K: 2000, The transient disappearance of cytokeratin in human fetal and adult ovaries. Anat Embryol (Berl).2000;201:207–15
  7. Benjamin E, Law S, Borrow LG. Intermediate filaments cytokeratin and vimentin in ovarian sex cord-stromal tumors with correlative studies in adult and fetal ovaries. J Pathol. 1987; 152(4):253–63.
  8. Czernobilsky B, Moll R, Levy R, Franke WW. Coexpression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur J Cell Biol.1985; 37:175–90.
  9.  Holthöfer H, Miettinen A, Lehto VP, Lehtonen E, Virtanen I. Expression of vimentin and cytokeratin types of intermediate filament proteins in developing and adult human kidneys. Lab Invest. 1984;50(5):552–9.
  10. Liu C-Y, Lin H-H, Tang M-J, Wang Y-K. Vimentin contributes to epithelial-mesenchymal transition cancer cell mechanics by mediating cytoskeletal organization and focal adhesion maturation. Oncotarget. 2015;6(18):15966-83.
  11. Nakopoulou L, Stefanaki k, Janinis J, Mastrominas M. Immunohistochemical Expression of placental alkaline phosphatase and vimentin in Epithelial Ovarian Neoplasms. Acta Oncologica. 1995;34(4): 511-5.
  12. Viale GGambacorta MDell'Orto PCoggi G.Coexpression of cytokeratins and vimentin in common epithelial tumours of the ovary: an immunocytochemical study of eighty-three cases. Virchows Arch A Pathol Anat Histopathol. 1988;413(2):91-101.
  13. Dabbs D. J. and Geisinger K R.Common epithelial ovarian tumors. Immunohistochemical intermediate filament profiles. Cancer.1988; 62: 368–74.
  14. Hou T, Liang D, He J, Chen X, Zhang Y. Primary peritoneal serous carcinoma: a clinicopathological and immunohistochemical study of six cases. International Journal of Clinical and Experimental Pathology. 2012;5(8):762-9.
  15.  Matsuzaki S, Darcha C. Epithelial to mesenchymal transition- like and mesenchymal to epithelial transition- like processes might be involved in the pathogenesis of pelvic endometriosis. Hum Reprod.2012;27:712-21 .
  16.  Kir G, Gurbuz A, Karateke A, Kir M. Clinicopathologic and immunohistochemical profile of ovarian metastases from colorectal carcinoma. World Journal of Gastrointestinal Surgery. 2010;2(4):109-16.
  17. Dabbs DJ, Geisinger KR, Noms HT. Intermediate filaments in endometrial and endocervical carcinomas: The diagnostic utility of vimentin patterns. Am J Surg Pathol 1986; 10368-576
  18. Borah T, Mahanta RK, Bora BD, Saikia S. Brenner tumor of ovary: An incidental finding. Journal of Mid-Life Health. 2011;2(1):40-1.