Iranian Journal of Pathology

The diagnostic value of TTF1, P63, HMWK [34βE12], CK7, and CD56 immunostaining in classification of lung carcinoma

Document Type : Original Research

Authors

Amir Hossein Jafarian
Masoumeh Gharib
Nema Mohammadian Roshan
Samaneh Sherafatnia
Abbas Ali Omidi
Sahar Bagheri

Dept. of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Iran

https://doi.org/10.30699/ijp.2017.25041
Abstract
Background & objective: The histologic distinction of small cell from non-small cell lung carcinoma and correct identification of all subtypes of lung carcinoma are very important in treatment management. The main method for histologic classification of lung tumors is based on morphology. However, in small bronchoscopic biopsies in particular, distinction is very difficult upon morphology alone. The current study aimed at evaluating the utility of a panel of antibodies, consisting of thyroid transcription factor (TTF-1), P63, high molecular weight keratin [HMWK (34βE12)], cytokeratin (CK7), and cluster of differentiation (CD56) for accurate distinction of bronchogenic carcinomas.
Methods: Bronchoscopic biopsies of 60 lung carcinoma cases including 20 small cell carcinomas, 20 adenocarcinomas, and 20 squamous cell carcinomas (SCCs) with typical morphologic features were selected. All these cases were immunohistochemically stained for TTF-1, P63, HMWK (34βE12), CK7, and CD56. All immunostained slides were scored as either positive or negative.
Results: The mean age of the patients was 60 years; ranged from 35 to 81. Sixteen patients were female and 44 were male. All adenocarcinomas were positive for CK7 and most of them (18/20; 90%) were positive for TTF-1. Most of small cell lung carcinomas were positive for TTF-1 (17/20; 85%), and CD56 (18/20; 90%). All squamous cell carcinomas (SCCs) were negative for TTF-1, but most of them were positive for HMWK (34βE12) and P63.
Conclusion: The obtained data showed that TTF-1, P63, CK7, CD56 and/or 34βE12 represent a useful panel of antibodies to identify lung carcinoma subtypes in small bronchoscopic biopsies.

Keywords

Immunohistochemistry
TTF1
P63
HMWK [34βE12]
CK7
CD56
lung carcinoma
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Iranian Journal of Pathology
Volume 12, Issue 3
Summer 2017
Pages 195-201

  • Receive Date 22 February 2016
  • Revise Date 10 July 2016
  • Accept Date 24 October 2016

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