Iranian Journal of Pathology

Validity of Selected WBC Differentiation Flags in Sysmex XT-1800i

Document Type : Original Research

Authors

Parya Bameni Moghaddam 1
Fatemeh Mahjoub 1
Amirhossein Emami 2
Alireza Abdollahi 3, 4

1 Dept. of Pathology, Tehran University of Medical Sciences, Tehran. Iran

2 Dept. of Internal Medicine, Tehran University of Medical Sciences, Tehran, Iran

3 Dept. of Pathology ,Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran

4 Thrombosis Hemostasis Research Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract
Background: Automatic Cell Counter devises make the CBC differential very easy and delivering the results in few second. However, the problem with this device is facing a flag requires a time-consuming microscopic review of the specimen which causes unacceptable wait times for patient as well as costs for laboratories. In this study, we calculated the validity of WBC diff flags in Sysmex XT-1800i. In addition, we verified the correlation between manual and automated samples.
Methods: Overall, 1095 flagged samples were selected in the period of 6 weeks (Imam Hospital complex, Tehran Iran, 2014). The results of both automated and manual counting of the samples were carefully studied and compared. Totally, 624 NRBC flags, 450 Blast flags, 155 abnormal WBC Scatter gram flags, 140 Eosinophilia flags and 468 Monocytosis flags were identified. Results: Considering NRBC and blast flags there was a significant difference between our manual counted and automated counted NRBCs and blasts (P<0.05). There was no significant difference between automated and manual counting of flags for WBC Scatter gram. A significant difference between automated and manual counting data in flags, eosinophilia and monocytosis was foun (P<0.05).
Conclusion: We propose the NRBC flags to be ignored and report negative except for the neonatal ward, and the Blasts flags to be ignored and report negative in all the cases. The WBC Scatter gram should be report positive. For eosinophilia and monocytosis flags, we propose, the Sysmex results should be considered correct and the manual checking would not be necessary.

Keywords

WBC differentiation
Sysmex cell count
WBC flags
NRBC
Blasts
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  • Receive Date 20 June 2015
  • Revise Date 14 February 2015
  • Accept Date 28 December 2015

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