Sakineh Amouian; Mahdi Farzadnia; Bahram Memar; Armin Attaranzadeh; Naser Tayyebi
Volume 3, Issue 1 , January 2008, , Pages 25-29
Abstract
Background and Objective: Evaluation of tumor proliferative activity may provide a predicting parameter to estimate biologic aggression and a subsequent prognosis that has been evaluated in many malignancies. We have selected renal cell carcinoma (RCC) in this study. To determine tumor proliferative ...
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Background and Objective: Evaluation of tumor proliferative activity may provide a predicting parameter to estimate biologic aggression and a subsequent prognosis that has been evaluated in many malignancies. We have selected renal cell carcinoma (RCC) in this study. To determine tumor proliferative activity, KI67 antibody was applied and results were compared with apoptosis, applying P53 antibody and using immunohistochemical staining. Patients and Methods: Specimens of 30 patients who underwent radical nephrectomy for RCC were selected for histopathology and immunohistochemical study. Two different grading systems (S&H, Fuhrman) were used to calibrate average nucleoli diameter and tumor grading on all specimens. After processing of paraffin-embedded samples, they were immunohistochemically stained applying (MIB-1) KI67 monoclonal and P53 antibodies. Then, statistical analysis was done. Results: Tumor grading correlated with the average nucleus diameter. Positive reaction to KI67 and P53 antibodies in tumors increased as compared to control group. No significant relationship between age, sex and tumor grade was obtained. Conclusion: These two antibodies are as easy and reliable markers that could be applied on formalin-fixed tissues for better assessment of the biologic behavior of RCC and probably prediction of patients’ outcome.
Hossein Ayatollahi; Maliheh Hasanzade; Mahdi Farzadnia; Mahdi Khabbaz Khoob; Atefeh Rahmanian
Volume 2, Issue 3 , June 2007, , Pages 100-104
Abstract
Background and Objective: The aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-CRP) in preeclampsia and to compare hs-CRP levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. Materials and Methods: Serum hs-CRP levels were ...
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Background and Objective: The aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-CRP) in preeclampsia and to compare hs-CRP levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. Materials and Methods: Serum hs-CRP levels were investigated in 40 cases of normal pregnant women, 37 cases with mild preeclampsia and 38 cases with severe preeclampsia in the third trimester of pregnancy. Venous blood samples were collected at admission to the hospital at least 6h before delivery for measurement of hs-CRP by immuno turbidometric method. The student t-test was used for comparison of proportions. Results: There were significant difference in the means serum hs-CRP between normal pregnant women and mild preeclamptic women (P<0.05). Serum concentration of hs-CRP were significantly higher in severe prceclampsia (p<0.05) than normal pregnancy. There were also significant differences in hs-CRP levels between mild and severe preclampsia (P<0.05). Conclusion: We found higher levels of hs-CRP in mild and severe preeclampsia than normal pregnancy and also these results suggest that hs- CRP are increased more in severe preeclamsia than mild preeclampsia , and may be useful in prediction and diagnosis of the severity of preeclampsia.
