TY - JOUR ID - 36629 TI - Molecular Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus in Iranian Burn Patients JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Tajik, Samira AU - Najar Peerayeh, Shahin AU - Bakhshi, Bita AU - Golmohammadi, Reza AD - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Y1 - 2019 PY - 2019 VL - 14 IS - 4 SP - 284 EP - 289 KW - Burn patients KW - Community-associated Staphylococcus aureus (CA-MRSA) KW - Methicillin resistant S. aureus (MRSA) KW - Molecular characterization DO - 10.30699/ijp.2019.94189.1917 N2 - Background & Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is reported as one of the important bacterial causes of burn wound infections. This study was carried out to investigate molecular characterization of community-associated MRSA (CA-MRSA) isolated from Iranian burn patients. Methods: A total of 31 isolates of S. aureus were collected from the Motahari Burns Hospital (Tehran, Iran) in 2016. All isolates were collected from outpatients and inpatients within 48 hours of admission. The mecA, pvl, tsst-1, hla-α, and psmα genes detecting, SCCmec, agr and PFGE typing were done. Result: A total of 13 (41.9%) isolates were cefoxitin-resistant and mecA-positive, which were considered as MRSA. The SCCmec typing MRSA strains revealed type II in 1 (7.7%), type III in 9 (69.2%), and other types in 3 isolates (23.7%) cases. The agr typing of all 31 isolates showed that 14 (45.2%), 1 (3.2%), 6 (19.4%), and 10 (32.3%) strains belonged to agr groups 1, 3, 4, and unknown type, respectively. The pvl, tsst-1, hla-α, and psmα genes were positive in 3 (9.7%), 4 (12.9%), 21 (67.7%), and 31 (100%) isolates, respectively. Considering the cut-off values of ≥50%, 3 groups of related isolates (cluster A1, B1, and C1) in PFGE study were observed. Conclusion: The MRSA strains of this study were initially isolated as Community-associated S. aureus (CA-MRSA); however molecular characterization showed that a significant proportion of them had hospital-associated MRSA (HA-MRSA) features. Therefore, it is likely that the HA-MRSA strains are spread among the community. UR - https://ijp.iranpath.org/article_36629.html L1 - https://ijp.iranpath.org/article_36629_dc931f5c6c8e25f173acc151a4ae02a2.pdf ER -