TY - JOUR ID - 28318 TI - The Effect of Smoking on Mast Cells Density and Angiogenesis in Chronic Periodontitis JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Jalayer naderi, Noushin AU - Semyari, Hasan AU - Hemmati, Reza AD - Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University AD - Department of Periodontic, Faculty of Dentistry, Shahed University AD - Faculty of Dentistry, Shahed University Y1 - 2017 PY - 2017 VL - 12 IS - 4 SP - 384 EP - 391 KW - Angiogenesis KW - Chronic periodontitis KW - Mast cell density KW - Cigarette smoking KW - Vascular Endothelial Growth Factor (VEGF) DO - 10.30699/ijp.2017.28318 N2 - Background and objective:Gingival bleeding reduction in smokers has been associated with decreased blood vessel density. The mechanism of suppressive effect of cigarette smoking on blood vessel density is not precisely defined. The aim of this study was to evaluate the impact of smoking on angiogenesis by assessing mast cells density and VEGF expression in chronic periodontitis. Materials& Methods: 52 paraffin embedded block of gingiva tissues with periodontitis obtained from 30 nonsmokers and 22 smokers undergoing flap surgery were examined immunohistochemically for VEGF expression. Mast cell counts was completed on toluidine blue stained slides. Exposure to cigarette smoking was calculated by the number of packs × year. Patients were classified into 4 groups based on the number of smoked cigarettes. The correlation between VEGF expression and mast cell counts was evaluated and compared in nonsmokers and smokers.  Results: The mean number of mast cells (p=0.004) and average value of VEGF expression (p = 0.000) in nonsmokers was significantly higher than smokers. No correlation was noted between VEGF expression / mast cell counts and number of smoked cigarettes in four groups of smokers (p=0.29,0.12 , 0.20  and 0.11, respectively). Conclusion: Mast cells and VEGF expression may account for suppressive effect of cigarette smoking on blood vessels in periodontitis. UR - https://ijp.iranpath.org/article_28318.html L1 - https://ijp.iranpath.org/article_28318_ad2beed2ee5504abb811d60eec159d4f.pdf ER -