TY - JOUR ID - 24218 TI - Investigation of Efflux-Mediated Tetracycline Resistance in Shigella Isolates Using the Inhibitor and Real Time Polymerase Chain Reaction Method JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Shahsavan, Shadi AU - Owlia, Parviz AU - Rastegar Lari, Abdolaziz AU - Bakhshi, Bita AU - Nobakht, Maliheh AD - Antimicrobial Resistance research center, Iran University of Medical science, Tehran, Iran AD - Molecular Microbiology Research Center, Shahed University, Tehran, IR Iran AD - Dept. of Microbiology, Faculty of Medicine, Iran University of Medical science, Tehran, Iran AD - Dept. of Microbiology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran AD - Antimicrobial Resistance research center, Iran University of Medical science, Tehran, Iran Y1 - 2017 PY - 2017 VL - 12 IS - 1 SP - 53 EP - 61 KW - Shigella KW - Tetracycline resistance KW - Efflux pump KW - Real-time PCR DO - 10.30699/ijp.2017.24218 N2 - Background: Shigella spp. are gram negative bacteria, which are of global public health importance. The growing of multidrug-resistant Shigella isolates are a major problemaround the world. Methods: Overall, 50 isolates of Shigella spp. from children diarrheic stools were studied. The isolates were identified and confirmed using biochemical, serological and molecular methods (ipaH, wbgZ and rfc genes). Antimicrobial susceptibility test was done according to the Clinical and Laboratory Standards Institute (CLSI) guidelines against minocycline, tetracycline, doxycycline, ampicillin, streptomycin, trimethoprim-sulfamethoxazole, nalidixic acid, norfloxacin, ciprofloxacin and levofloxacin. Also, the role of efflux pump in defense of Shigella against tetracycline was investigated by Minimum Inhibitory Concentration (MIC) with and without an efflux pump inhibitor. Detection of tetA, tetB, tetC and tetD genes in Shigella was evaluated by conventional Polymerase Chain Reaction (PCR) and real time PCR. Results: Molecular identification revealed a prevalence of 14% for Shigella flexneri and 86% for Shigella sonnei. Minimum Inhibitory Concentration (MIC) of 90% of resistant isolates was changed in the presence CCCP.Results of conventional PCR exhibited that 66% of isolates were positive for tetA, while according to real time PCR method, 90% of isolates carried tetA. Positive results for tetB were12% and 18% by conventional and real time PCR methods, respectively. No positive results were detected for tetC and tetD. Also, tetB was detected only in S. flexneri while tetA was detected in both S. flexneri and S. sonnei. Conclusion: It seems that efflux-mediated tetracycline resistance to tetracycline in S. flexneri can be related to tetB, however resistance in S. sonnei can be related to the expression of tetA. UR - https://ijp.iranpath.org/article_24218.html L1 - https://ijp.iranpath.org/article_24218_ace747ab61f9851a9e3b65312bf0ea2c.pdf ER -