Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Prevalence of Clinically Isolated Metallo-beta-lactamase-producing Pseudomonas aeruginosa, Coding Genes, and Possible Risk Factors in Iran
1
9
EN
Abdolmajid
Ghasemian
Microbiology Depat, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
a.ghasemian@modares.ac.ir
Kobra
Salimian Rizi
Dept. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Hassan
Rajabi Vardanjani
Researcher of Shahrekord University of Medical Sciences, Shahrekored, Iran
Farshad
Nojoomi
Microbiology Dept, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
fnojoomi2@gmail.com
10.30699/ijp.13.1.1
<strong><em>Background & Objective</em></strong><strong>: </strong>The spread of carbapenem-resistant <em>Pseudomonas aeruginosa</em> is a global concern. Metallo-beta-lactamase (MBL) enzymes cause extensive drug resistance among Gram-negative bacteria. The current study aimed at determining the prevalence of MBL-producing <em>P. aeruginosa</em> in Iran.<br /> <strong><em>Methods</em></strong><strong>:</strong> A total of 43 studies were found out of which 36 were adopted. Data were collected from Google, Google Scholar, Science Direct, PubMed, Scopus, Embase, and Sciverse. The terms “<em>Pseudomonas</em> <em>aeruginosa</em>”, “metallo-beta-lactamase”, “prevalence”, “carbapenems”, and “Iran” were searched. Data from the isolates not producing MBLs were excluded from the study. Data were analyzed with Graph Pad Prism 6, meta-analysis section.<br /> <strong>Results:</strong> According to the results of the current study, 36 surveys indicated that 55% of the clinically isolated <em>P. aeruginosa</em> in Iran were resistant to imipenem and meropenem, among which 37.72% were the MBL producers. Among genes encoding MBLs, <em>bla<sub>VIM</sub></em> and <em>bla<sub>IMP</sub></em> were predominant with the prevalence of 12.91%±11.01% and 12.50%±23.56%, respectively. No report of harboring <em>bla</em><sub>NDM1</sub> and bla<sub>SPM1</sub> by <em>P. aeruginosa</em> was found, similar to most of the other countries in Asia. The prevalence of <em>bla<sub>VIM</sub></em> and <em>bla<sub>IMP</sub></em> from burn settings were 11.50%±3.5% and 24.65%±23%, respectively. Furthermore, the prevalence of these genes was not significantly different among burn and non-burn isolates (P=0.942 and P=0.597, respectively). Moreover, no relationship was observed between the MBL production and patients’ age range.<br /> <strong>Conclusion:</strong> Approximately half of <em>P. aeruginosa</em> isolates were carbapenem-resistant in Iran, and approximately half were the MBL producers. The <em>bla<sub>VIM </sub></em>and <em>bla<sub>IMP</sub></em> were the predominantMBLs among <em>P</em>. <em>aeruginosa</em> strains, while other genes were not found in <em>P. aeruginosa</em>. Moreover, there was no significant difference between <em>bla<sub>VIM</sub></em> and <em>bla<sub>IMP</sub></em>among burn and non-burn isolates. Due to the multiple drug resistance conferred by MBLs, detection and control of their spread alongside proper therapeutic regimens in hospitals and community settings are essential to prevent infection acquisition.
Pseudomonas aeruginosa,Metallo-Beta-Lactamase,Carbapenems,Iran
https://ijp.iranpath.org/article_29936.html
https://ijp.iranpath.org/article_29936_f447aaa49044781325728fe83c4a5e8a.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis
10
22
EN
Rayhaneh
Ahangar-Parvin
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
r.parvin92@yahoo.com
Marzieyeh
Mohammadi-Kordkhayli
Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
m_mshomal@yahoo.com
Sayyed Vahab
Azizi
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
vahab.azizi1363@yahoo.com
Maryam
Nemati
Dept. of Laboratory Sciences, Para-Medical School, Kerman University of Medical Sciences, Kerman, Iran
nemati_m22@yahoo.com
Hossian
Khorramdel-Azad
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
khorramdelazad@gmail.com
Zahra
Taghipour
Dept. of Histology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
taghipourz@yahoo.com
Zuhair
Hassan
Dept. of Immunology, Medical School, Tarbiat Moddares University, Tehran, Iran
hasan_zm@modares.ac.ir
Seyed Mohammad
Moazzeni
Dept. of Immunology, Medical School, Tarbiat Modares University, Tehran, Iran
moazzeni@modares.ac.ir
Abdollah
Jafarzadeh
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
jafarzadeh14@yahoo.com
10.30699/ijp.13.1.10
<strong><em>Background & objective</em></strong> The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE).<br /> <strong><em>Methods</em></strong><strong>:</strong> EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively.<br /> <strong><em>Results</em></strong><strong>:</strong> IL-12 gene expression and its serum levels in PBS-injected- or OO-administrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected- or OO-administrated EAE groups (P <strong><em>Conclusion</em>:</strong> VD modulates the expression of IL-12 and TGF-β in spinal cord and serum of EAE mice.
Experimental Autoimmune,Encephalomyelitis,Vitamin D,IL-12,TGF-β
https://ijp.iranpath.org/article_29937.html
https://ijp.iranpath.org/article_29937_a54c4d3157b9545cdcc5c422b94a3f23.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Immunohistochemical Characterization of Normal Ovary and Common Epithelial Ovarian Neoplasm with a Monoclonal Antibody to Cytokeratin and Vimentin
23
29
EN
Ankita
Goel
Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore, India
ankigoel88@gmail.com
Nandam
Rao
Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore, India
nmr2020kmc@gmail.com
Vissa
Santhi
Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore
santhijp@gmail.com
Syam
Byna
Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore, India
syam.byna@gmail.com
Bhavana
Grandhi
Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore, India
drbhavana.grandhi@gmail.com
Jyothi
Conjeevaram
Dept. of preventive and social medicine,Narayana medical college and hospital, Nellore, India
jyothi.conjeevaram@gmail.com
10.30699/ijp.13.1.23
<strong><em>Background & Objective</em></strong><strong>: </strong>The common epithelial ovarian tumors are classified into serous, mucinous, clear cell, endometrioid, the Brenner, mixed, and undifferentiated types. Cytoskeleton intermediate filament composition of ovarian tissues indicates that the cytokeratin and vimentin are observed in ovarian surface epithelium along with the common ovarian epithelial tumors. The current study aimed at investigating the cytokeratin and vimentin expression in epithelial ovarian tumors to establish a diagnostic relevance.<br /> <strong><em>Methods</em></strong><strong>:</strong> Sixty-six common epithelial ovarian tumors were studied using anti-cytokeratins (Monoclonal Mouse Anti-Human Cytokeratin Clones AE1/AE3; DAKO, Denmark,) and anti-vimentin (Monoclonal Mouse Anti-Vimentin, Clone V9; DAKO, Denmark,) to ascertain the intermediate filament profiles in formalin-fixed and paraffin-embedded surgical pathology materials.<br /> <strong><em>Results:</em></strong> All ovarian epithelial tumors expressed cytokeratin in a uniform fashion. Vimentin was coexpressed with high intensity in 62.5% of serous carcinomas, mild intensity in 25% of mucinous adenocarcinoma, and moderate intensity in single case of endometrioid adenocarcinoma. Vimentin decoration in mucinous carcinoma had a focal involvement, whereas malignant endometrioid and serous decoration tended to involve larger areas. There was a significantly increased expression of vimentin in serous cystadenoma and serous carcinoma, compared with their mucinous counterparts. Also, vimentin expression and histologic grade of serous tumors showed a positive correlation. No association was found between vimentin expression and degree of differentiation in mucinous, endometrioid, and Brenner tumors.<br /> <strong><em>Conclusion:</em></strong> The current investigation emphasized the efficiency of immunohistochemistry (IHC) typing as a tool for a more precise characterization of the origin and differentiation of human neoplasms.
Cytokeratin,Vimentin,Epithelial Tumor,Ovary,Benign,Borderline,Malignant
https://ijp.iranpath.org/article_29938.html
https://ijp.iranpath.org/article_29938_fef2336931be5c60e53b8d171126bc94.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Micronucleus Assay in Exfoliated Buccal Epithelial Cells Using Liquid Based Cytology Preparations in Building Construction Workers
31
38
EN
ARUL
P
Dept. of Pathology, Dhanalakshmi Srinivasan Medical College and Hospital, Siruvachur, Perambalur, Tamilnadu-621113, India
drarul3@gmail.com
Shetty
Smitha
Dept. of Pathology, Dhanalakshmi Srinivasan Medical College and Hospital, Siruvachur, Perambalur, Tamilnadu-621113, India
smitha@gmail.com
Suresh
Masilamani
Dept. of Pathology, Dhanalakshmi Srinivasan Medical College and Hospital, Siruvachur, Perambalur, Tamilnadu-621113, India
jakstatms@gmail.com
Akshatha
C
Dept. of Pathology, Dhanalakshmi Srinivasan Medical College and Hospital, Siruvachur, Perambalur, Tamilnadu-621113, India
akshatha.yashas@gmail.com
10.30699/ijp.13.1.31
<strong><em>Background and objective:</em></strong>Cytogenetic damage in exfoliated buccal epithelial cells due to environmental and occupational exposure is often monitored by micronucleus (MN) assay using liquid based cytology (LBC) preparations. This study was performed to evaluate MN in exfoliated buccal epithelial cells of building construction workers using LBC preparations.<br /> <strong><em>Material and methods:</em></strong> LBC preparations of exfoliated buccal epithelial cells from 100 subjects [50 building construction workers (cases) and 50 administrative staffs (controls)] was evaluated by May-Grunwald Giemsa, Hematoxylin and Eosin and Papanicolaou stains. Student’s t test was used for statistical analysis and a P value of <strong><em>Results:</em></strong> The mean frequencies of MN for cases were significantly higher than controls regardless of staining methods used.There were statistically significant differences between smokers and non-smokers of the controls as well as duration of working exposure (5 years) and smokers and non-smokers of cases (P=0.001). However, there were meaningful differences regarding mean frequencies of MN between smokers, non-smokers, those with alcohol consumption or not in cases and controls using various stains (P=0.001).<br /> <strong><em>Conclusion:</em> </strong>There was an increased risk of cytogenetic damage in building construction workers. However, evaluation of MN of exfoliated buccal epithelial cells in building construction workers serve as a minimally invasive biomarker for cytogenetic damage. LBC preparations can be applied for MN assay as it improves the quality of smears and cell morphology, decreases the confounding factors and reduces false positive results.
Liquid Based Cytology Preparations,Exfoliated Buccal Epithelial Cells,Micronucleus,Building construction,Workers
https://ijp.iranpath.org/article_29939.html
https://ijp.iranpath.org/article_29939_c9c83dcc1a3f34584acdcbce1ad07e08.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
The Evaluation of Diagnostic and Predictive Values of Helicobacter pylori Stool Antigen Test in Iranian Patients with Dyspepsia
39
44
EN
Fahimeh
Safarnezhad Tameshkel
0000-0001-8953-5447
Gastrointestinal & Liver Disease Research Center (GILDRC), Iran University of Medical Sciences, Tehran, Iran
fahime.1615@yahoo.com
Mohammad Hadi
Karbalaie Niya
0000-0003-4169-6932
Dept. of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
mohamad.karbalai@yahoo.com
Zahedin
Kheyri
Dept. of Internal Medicine, Amiralmomenin hospital, Arak University of Medical Sciences, Arak, Iran
asal.1615@yahoo.com
Davood
Azizi
Dept. of Pathology, Amiralmomenin hospital, Arak University of Medical Sciences, Arak, Iran
karbalai.m2011@yahoo.com
Farzin
Roozafzai
Gastrointestinal & Liver Disease Research Center (GILDRC), Iran University of Medical Sciences, Tehran, Iran
Samaneh
Khorrami
Dept. of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
dr.mhkn@yahoo.com
10.30699/ijp.13.1.39
<strong><em>Background and Objectives</em></strong><strong>:</strong> Iran, as a developing country, is experiencing high burdens of <em>Helicobacter pylori</em> (Hp)-associated non-communicable diseases. Hp stool antigen test (HpSA) is widely used as an inexpensive and feasible noninvasive method to diagnose Hp infection, instead of invasive approaches. The current study aimed at evaluating the diagnostic and predictive values of HpSA test for Hp infection in Iranian patients with dyspepsia.<br /> <strong><em>Materials and methods:</em></strong> The current cross sectional study was performed on 100 patients with dyspepsia. Gastric mucosal specimens were taken, processed, and examined according to the standard protocols. Simultaneously, stool samples were obtained and sent to laboratory for further analyses. Hp stool antigen titers were assessed using enzyme-linked immunosorbent assay (ELISA) technique.<br /> <strong><em>Results:</em></strong>Stool antigen titers were not associated with gender (P-value=0.284), but correlated to age (r=0.213, P-value=0.034).Considering0.385 as a cutoff point, the HpSA test had 80.4% sensitivity and 85.7% specificity.<br /> <strong><em>Conclusion:</em> </strong>Based on cost-effectiveness of HpSA test, the current study findings corroborated the use of HpSA test to detect and follow-up patients with Hp infection, as an alternative method to detect Hp rather than invasive procedures.
Helicobacter pylori,Stool Antigen Test,Sensitivity,Specificity
https://ijp.iranpath.org/article_29940.html
https://ijp.iranpath.org/article_29940_00c316b5a9d4ff081c80d46a6de56ea3.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Expression of Prostate-Specific Membrane Antigen (PSMA) in Brain Glioma and its Correlation with Tumor Grade
45
53
EN
Hiva
Saffar
0000-0003-1489-9611
Dept. of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Iran
hsaffar@sina.tums.ac.ir
Maryam
Noohi
Dept. of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Iran
maryam.noohi@yahoo.com
Seyed Mohammad
Tavangar
0000-0002-4253-2385
Dept. of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Iran
tavangar@ams.ac.ir
Hana
Saffar
Dept. of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Iran
hana_saffar283@yahoo.com
Sima
Azimi
Dept. of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Iran
parisima.azimi@gmail.com
10.30699/ijp.13.1.45
<strong><em>Background & Objective</em></strong> Angiogenesis is an essential component of tumor growth. Expression of PSMA on the neo-vasculature of many solid tumors, including glioblastoma multi-form, has been determined. The pattern of expression suggests that PSMA may play a functional role in angiogenesis.<br /> <strong><em>Methods</em></strong><strong>:</strong> expression of PSMA in different grades of brain glioma was evaluated by the immunohistochemistry method to determine the probable usefulness of anti-PSMA antibody as complementary target therapy in different grades of glioma.<br /> <strong><em>Results:</em></strong> Overall, 72 cases of low (grade I and II) and high (grade III and IV) grade gliomas were evaluated for expression of PSMA. Positive PSMA staining was observed in 12 (33.3%) of high grade and 3 (8.3%) of low grade gliomas. Although, high grade tumors more commonly had positive result for PSMA (P value=0.009), the intensity of staining was significantly stronger in low-grade tumors (P value=0.009).<br /> <strong><em>Conclusion:</em></strong> Expression of PSMA in different grades of glioma might provide a basis for further investigations focusing on selective target therapy in combination with the current standard care in all glioma grades, to improve treatment efficacy.
Prostate-Specific Membrane Antigen,Glioma,Grade,Tumor angiogenesis
https://ijp.iranpath.org/article_29941.html
https://ijp.iranpath.org/article_29941_2151e22df1c038e1f6ea159438c8ad59.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses
54
62
EN
Mohsen
Nakhaie
Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
mohsennakhaee1367@gmail.com
Hoorieh
Soleimanjahi
Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
soleim_h@modares.ac.ir
Hamid Reza
Mollaie
0000-0001-6874-0011
Dept. of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran
hamid2008kmu@gmail.com
Seyed Mohamad Ali
Arabzadeh
Dept. of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran
a_arabzadeh@kmu.ac.ir
10.30699/ijp.13.1.54
<strong><em>Background and objective:</em></strong>Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. The Multiplex RT PCR method was the preferred method for the detection of influenza A, B, and adenoviruses in clinical specimens because it is rapid, sensitive, specific, and more cost-effective than alternative methods<br /> <strong><em>Methods:</em></strong>After collecting samples from patients with respiratory disease, virus genome was extracted, then Monoplex PCR was used on positive samples and Multiplex RT-PCR on clinical specimens. Finally, by comparing the bands of these samples, the type of virus in the clinical samples was determined.<br /> <strong><em>Results:</em></strong>Performing Multiplex RT-PCR on 50 samples of respiratory tract led to following results; flu A: 12.5%, fluB: 50%, adeno: 27.5%, negative: 7.5%, and 2.5% contamination.<br /> <strong><em>Conclusion: </em></strong>Reverse transcription-multiplex Polymerase Chain Reaction (PCR) technique, a rapid diagnostic tool, has potential for high-throughput testing. This method has a significant advantage, which provides simultaneous amplification of numerous viruses in a single reaction. This study concentrates on multiplex molecular technologies and their clinical application for the detection and quantification of respiratory pathogens. The improvement in diagnostic testing for viral respiratory pathogens effects patient management, and leads to more cost-effective delivery of care. It limits unnecessary antibiotic use and improves clinical management by use of suitable treatment.
Polymerase chain reactionn,Influenza A,Influenza B,Adenovirus,Multiplex RT-PCR,Respiratory infection
https://ijp.iranpath.org/article_29942.html
https://ijp.iranpath.org/article_29942_8d65e84f4404bdb62db86fb782aba8e7.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
The Association between Ocular Problems and Serum Testosterone, Prolactin and Thyroglobulin Concentrations in Delayed Phase of Sulfur Mustard Exposure
63
70
EN
Hassan
Ghasemi
Immunoregulation Research Center, Shahed University, Tehran, I.R. Iran
ghasemi518@yahoo.com
Nayere
Askari
Dept. of biology, Faculty of Basic Sciences, Shahid Bahonar University of Kerman, Kerman, I.R Iran
askari.nr@gmail.com
Sakine
Moaiedmohseni
Immunoregulation Research Center, Shahed University, Tehran, I.R. Iran
smmohseni@yahoo.com
Soghrat
Faghihzadeh
Dept. of Biostatistics and Social Medicine, Zanjan University of Medical Sciences, Zanjan, I.R. Iran
s.faghihzadeh@zums.ac.ir
Susan
Kabudanian Ardestani
Institute of Biochemistry and Biophysics, Tehran University, Tehran, I.R. Iran
ardestany@ut.ac.ir
Elham
Faghihzadeh
Immunoregulation Research Center, Shahed University, Tehran, I.R. Iran
elham-fagh@yahoo.com
Tooba
Ghazanfari
Immunoregulation Research Center, Shahed University, Tehran, I.R. Iran
tghazanfari@yahoo.com
10.30699/ijp.13.1.63
<strong><em>Background and objective</em></strong> Aside from direct toxic effects, Sulfur Mustard (SM) induced serum hormone abnormalities may aggravate ocular complaints, including Ocular Surface Discomfort (OSD) (burning, itching, and redness), dry eye sensation, photophobia, blurred vision, foreign body sensation, and pain. The aim of the current study was to investigate the possible association of ocular complaints with serum hormone concentrations in chronic phase of Sulfur Mustard (SM) exposure.<br /> <strong><em>Methods:</em></strong>As a part of Sardasht Iran Cohort Study (SICS), 372 SM-exposed patients and 128 non-exposed participants were enrolled. Ocular complaints and ocular surface biomicroscopic conditions and serum hormones were compared.<br /> <strong><em>Results:</em></strong>The exposed with tearing group had significantly higher mean serum levels of testosterone and prolactin (ng/mL) than controls (5.75 vs. 4.75, P=0.031; 11.71 vs. 8.42, P=0.009). The exposed with OSD group had significantly higher mean serum levels of prolactin than controls (12.48 vs. 6.90, P=0.002). The exposed with photophobia group had significantly higher mean serum levels of testosterone than the matched exposed (6.25 vs. 5.65, P=0.013). The exposed with blurred vision group had significantly higher mean serum levels of Thyroglobulin (Tg) (ng/mL) than the matched exposed (65.73 vs. 32.6, P=0.003).<br /> <strong><em>Conclusion:</em> </strong>Higher mean serum levels of testosterone (in exposed with tearing and photophobia) and prolactin (in exposed with tearing and OSD) may play protective roles against SM effects. Higher mean serum levels of Tg may deteriorate the tear film integrity and optical surface, which causes blurred vision. In the chronic phase of SM toxicity, some ocular surface problems are associated with alterations in the serum concentrations of testosterone, prolactin, and Tg.
Sulfur mustard,Eye Injuries,Serum,Hormones
https://ijp.iranpath.org/article_29943.html
https://ijp.iranpath.org/article_29943_a68fea38fe4bdd65ba3517805b76e3cf.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Prognostic Significance of Reduction in Ki67 Index After Neoadjuvant Chemotherapy in Patients With Breast Cancer in Kerman Between 2009 And 2014
71
77
EN
Vahid
Moazed
Hematology and Oncology Division, Dept of Internal medicine, Afzalipour Kerman Medical Science University, Kerman, Iran
moazed_vahid@yahoo.com
Elham
Jafari
0000-0001-9240-9177
Pathology and Stem Cells Research Center, Dept of Pathology, Afzalipour Kerman Medical Science University, Kerman, Iran
ejfarda@yahoo.com
Behjat
Kalantari khandani
Hematology and Oncology Division, Dept of Internal medicine, Afzalipour Kerman Medical Science University, Kerman, Iran
bkalantarikhandani@gmail.com
Ali
Nemati
Hematology and Oncology Division, Dept of Internal medicine, Afzalipour Kerman Medical Science University, Kerman, Iran
alinemati76@yahoo.com
seyedamir
benrazavi
Dept. of Internal Medicine, Afzalipour Kerman Medical Science University, Kerman, Iran
drbenrazavi@yahoo.com
10.30699/ijp.13.1.71
<strong><em>Background and objective:</em></strong>Breast cancer is the most common malignancy among women. The Neoadjuvant chemotherapy is the treatment of choice for non-operable tumors. The Ki67 is a proliferation marker that can be used to predict the therapeutic response to chemotherapy and the patients' prognosis.<br /> <strong><em>Methods:</em></strong> This retrospective study was carried out on 55 consecutive patients with breast cancer referred to a Training Tertiary Healthcare Center in Kerman, Iran since 2009 to 2014. After diagnostic approval, the tissue samples of patients were examined for estrogen and progesterone receptors, ki67 and HER2-neu markers by using immunohistochemical staining. Then the patients were treated with 6 cycles of Neoadjuvant chemotherapy regimens by Doxorubicin and Taxans or 4 chemotherapy cycles, containing Anthracycline and Cyclophosphamide and 4 cycles of Paclitaxel. After mastectomy, their samples were reexamined for ki67 again and classified into three groups (low: ki67<15%), medium (Ki67 = 16-30%) and high (Ki67> 30%).<br /> <strong><em>Results:</em></strong> Before chemotherapy, 54.5% of the patients had high expression of Ki67. But after chemotherapy, 52.7 of the patients had complete therapeutic response showing that the Ki67 level was reduced significantly (P=0.003).<br /> <strong><em>Conclusion:</em> </strong>Before and after Neoadjuvant chemotherapy, Ki67 measurements may be used as a predictive marker of therapeutic response.
Neoadjuvant Therapy,Ki 67,Breast cancer,Prognosis
https://ijp.iranpath.org/article_29944.html
https://ijp.iranpath.org/article_29944_5c2fba9243efb69816ca74a185acec5b.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Beware of Bone Marrow: Incidental Detection and Primary Diagnosis of Solid Tumours in Bone Marrow Aspiration and Biopsies; A Study of 22 Cases
78
84
EN
Ruchita
Tyagi
Dept. of Pathology, Dayanand Medical College and Hospital, Tagore Nagar, Ludhiana, India
ruchitatyagi@gmail.com
Aminder
Singh
0000-0003-2015-2354
Dept. of Pathology, Dayanand Medical College and Hospital, Tagore Nagar, Ludhiana, India
dramisingh@gmail.com
Bhavna
Garg
Dept. of Pathology, Dayanand Medical College and Hospital, Tagore Nagar, Ludhiana, India
dr.bhavnagarg@yahoo.co.in
Neena
Sood
Dept. of Pathology, Dayanand Medical College and Hospital, Tagore Nagar, Ludhiana, India
drneena_sood@yahoo.co.in
10.30699/ijp.13.1.78
<strong><em>Background & objective</em></strong><em>: </em>Introduction: First detection of any solid tumour as metastatic deposits in bone marrow directs clinicians to start searching for the primary tumour. Detection of bone marrow metastasis determines the stage of the malignancy, prognosis, mode of treatment, chemotherapeutic response and follow-up in case of relapse. The aim of the current study was to analyse the clinico-haematological presentation and morphological pattern of infiltration of solid tumours detected first as metastatic deposits on bone marrow examination.<br /> <strong><em>Methods</em></strong><strong><em>:</em></strong>Three-year retrospective analysis of MGG-stained bone marrow aspiration smears and touch imprints of the bone marrow biopsy and Hematoxylin and Eosin (H&E) stained histopathological sections of biopsies was performed at the Department of Pathology at a tertiary care institute (January 2014 to December 2016). The morphological pattern of metastatic deposits and haematological profiles of the patients were analysed. Exclusion criterion was the presence of hematolymphoid malignancies.<br /> <strong><em>Results</em></strong><strong><em>:</em></strong> In 22 cases, bone marrow was the first site of detection of metastasis. The age of the patients ranged from 3 years and 10 months to 82 years, with equal gender predilection. Overall, 16 cases had cytopenias, 9 cases each had leucocytosis and leukoerythroblastic presentation. The metastasis was from Ewing’s sarcoma, prostate carcinoma, gastric adenocarcinoma invasive duct carcinoma breast, gallbladder carcinoma, lacrimal duct carcinoma and invasive papillary urothelial carcinoma.<br /> <strong><em>Conclusions: </em></strong>Bone marrow examination is a cheap and reliable investigation to detect metastasis in an unsuspecting case. Bilateral trephine biopsies are recommended to increase the efficacy of detecting bone marrow metastasis.
Bone marrow aspiration,Biopsy,Metastatic Deposit,Primary Diagnosis
https://ijp.iranpath.org/article_30048.html
https://ijp.iranpath.org/article_30048_f0a92d3b32d92990a4cd607832167d7c.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Sinonasal Teratocarcinosarcoma- A Rare Tumour not so Rarely Misdiagnosed
85
88
EN
Jayalakshmy
Leelamma
Dept. of Pathology, Government Medical College, Kottayam, Kerala. India
jayafloyyd@gmail.com
Bhavya
P. Mohan
Dept. of Pathology, Government Medical College, Kottayam, Kerala. India
jayafloyyd80@gmail.com
Aparna
Srinivasan
Dept. of Pathology, Government Medical College, Kottayam, Kerala. India
albrin.james@gmail.com
10.30699/ijp.13.1.85
Sinonasal teratocarcinosarcoma (STCS) is a rare, morphologically heterogeneous and highly aggressive tumour of ambiguous origin. It is characterized by the presence of benign and malignant epithelial, mesenchymal and neuroectodermal components. Because of their rarity and heterogeneity, these lesions are often misdiagnosed, leading to management difficulties. Adequate sampling with a high index of suspicion is needed to diagnose this rare tumour. We reported here a 48-year old man with right nasoethmoidal mass eroding the cribriform plate with intracranial extension. An initial incisional biopsy was performed and a diagnosis of craniopharyngioma was made. Subtotal endoscopic excision of the mass revealed features of STCS. Immunohistochemistry confirmed the same. The patient was subsequently treated with radiotherapy. The histogenesis, histopathological features, immunohistochemistry findings, clinical features and treatment were discussed here. Till date, there are less than 100 cases reported in English literature.
Sinonasal,Teratocarcinosarcoma,heterogeneous
https://ijp.iranpath.org/article_30049.html
https://ijp.iranpath.org/article_30049_f5929ebc035f0d46a0b10bbb28f47ab5.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Sarcomatoid Carcinoma of Renal Pelvis Involving Ureter and Renal Parenchyma with Heterologous Osteosarcomatous Differentiation: A Case Report and Review of Literature
89
93
EN
Bhavya
P.Mohan
Dept. of Pathology, Government Medical College, Kottayam, Kerala, India
bhavyasharath80@gmail.com
Jayalakshmy
PL
Dept. of Pathology, Government Medical College, Kottayam, Kerala, India
jayafloyyd@gmail.com
V
Letha
Dept. of Genitourinary Surgery, Government Medical College, Kottayam, Kerala, India
drlethav@gmail.com
Suresh
Bhat
Dept. of Pathology, Government Medical College, Kottayam, Kerala, India
drsureshbhat@yahoo.com
10.30699/ijp.13.1.89
Sarcomatoid carcinoma is a high‑grade rare malignant tumor with both epithelial and mesenchymal components. Sarcomatoid carcinoma in the upper urinary tract is very rare. We reported here a case of sarcomatoid carcinoma of renal pelvis with osteosarcomatous differentiation, with involvement of the ureter and renal parenchyma in a 68-year-old female. Histologically, predominant pleomorphic spindle cell sarcoma component with osteoid production and urothelial carcinoma component with in situ areas were identified. Immunohistochemical analysis showed vimentin positivity in sarcomatous component and cytokeratin positivity in carcinomatous component.
Sarcomatoid Carcinoma,renal pelvis,Osteosarcoma
https://ijp.iranpath.org/article_30050.html
https://ijp.iranpath.org/article_30050_9f1d60aa9103a33f2b0c4dc5475b942d.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Pseudopapillary and Macrofollicular Microscopic Growth Patterns in an Advanced Stage Ovarian Dysgerminoma: A Case Report
94
98
EN
Fatemeh
Nili
0000-0002-9835-897X
Dept. of Pathology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
f-nili@sina.tums.ac.ir
Niusha
Noubari
Dept. of Pathology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
n.noubari@yahoo.com
Alireza
Abdollahi
0000-0002-5714-967X
Dept. of Pathology, Imam Hospital Complex, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
dr_p_abdollahi@yahoo.com
10.30699/ijp.13.1.94
ysgerminoma is one of the two most common types of ovarian germ cell tumors. Providing accurate pathologic diagnosis and treatment planning, the prognosis is good even in advanced stages. Pathologic diagnosis is generally straightforward. In microscopic examination, the usual known growth patterns in tumor cells are solid, trabecular, insular and rarely pseudoglandular.<br /> In this paper, we reported an advanced ovarian dysgerminoma with different microscopic patterns of growth, including pseudopapillary and macrofollicular structures, in an 18-year-old woman. The patient underwent staging laparotomy and is currently receiving chemotherapy.
Dysgerminoma,Microscopic Growth Pattern,Pseudopapillary,Macrofollicular
https://ijp.iranpath.org/article_30051.html
https://ijp.iranpath.org/article_30051_49bd149a864507d18d0e97f9a7de03b0.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Ovarian Small Cell Carcinoma: A Rare Case Report and Review of Literature
99
102
EN
Savita
Agarwal
Dept. of Pathology, UP University of Medical Sciences, Saifai, Etawah, India
megharalli_86@yahoo.co.in
Megha
Ralli
Dept. of Pathology, UP University of Medical Sciences, Saifai, Etawah, India
drmegharalli@gmail.com
Pinki
Pandey
Dept. of Pathology, UP University of Medical Sciences, Saifai, Etawah, India
pnkdxt@gmail.com
Shruti
Singh
Dept. of Pathology, UP University of Medical Sciences, Saifai, Etawah, India
drshruti137@gmail.com
10.30699/ijp.13.1.99
Ovarian small cell carcinoma is a rare and highly malignant tumor with poor prognosis. It usually presents in younger females with features of hypercalcemia. The exact histogenesis of the tumor is unclear and it may present as an undifferentiated tumor. In such cases, immunohistochemistry (IHC) plays an important role to confirm the diagnosis. Limited treatment options are available and mainly include radical surgery followed by adjuvant therapy in advanced stages. The current report was a rare case of small cell carcinoma of ovary presenting no symptoms of hypercalcemia diagnosed on histopathological examination and IHC findings.
Small Cell Carcinoma,Immunohistochemistry,Ovary
https://ijp.iranpath.org/article_30052.html
https://ijp.iranpath.org/article_30052_744d4faca18cd302c4eb901f09faeb2c.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Ectopic Thyroid in Stomach; a Case Report
103
105
EN
Parvin
Rajabi
Dept. of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
p-rajabi@med.mui.ac.ic
Seyed Mehdi
Eftekhari
Dept. of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
seyedmehdieftekhari@med.mui.ac.ir
Elham
Rouhani
Dept. of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
ehamrouhani@yahoo.com
Omid
Mirmosayyeb
Dept. of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
omid.mirmosayyeb@gmail.com
Azar
Baradaran
0000-0001-7058-6367
Dept. of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
azarbaradaran@yahoo.com
10.30699/ijp.13.1.103
An ectopic thyroid is a form of thyroid dysgenesis in which an entire or parts of the thyroid gland may be located in another part of the body than what is the usual place. The most frequent location is the base of tongue. Although most cases are asymptomatic, symptoms related to tumor size and its relationship with surrounding tissues, hormonal dysfunction and seldom malignancy may also occur. Any disease affecting the thyroid gland may involve the ectopic thyroid, including malignancy.
Ectopic thyroid,Thyroid Dysgenesis,Hormonal Dysfunction,Stomach
https://ijp.iranpath.org/article_30053.html
https://ijp.iranpath.org/article_30053_7566b6f4fb89d538ca329c4fad0ac2d8.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
13
1
2018
01
01
Reporting an Experience: Improving the Feulgen Staining Technique for Better Visualizing of Nucleus
106
107
EN
Noushin
Jalayer naderi
0000-0002-7356-3205
Dept. of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University, Tehran, Iran
noushin_jly85@yahoo.com
10.30699/ijp.13.1.106
Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in hematoxylin for 3-5 minutes in final step after staining the slides with light green, provides a greater ground contrast and very better visualizing of nucleus. By this method, a clear distinction can be made between micronuclei and other structures suggestive of a micronucleus.
Feulgen staining,Staining technique,Nucleus,Nuclear abnormalities
https://ijp.iranpath.org/article_30054.html
https://ijp.iranpath.org/article_30054_a4ea3c6c5d2095028edcc80c84fd8475.pdf