ORIGINAL_ARTICLE
Relationships of Serum Level of High- Sensitivity C- Reactive Protein (hs- CRP) and White Blood Cell (WBC) Count with Gamma- Glutamyltransferase among Iranian Healthy Adults
Background & Objective: There are many common infections and inflammations among people over the world. This demands an affordable and reliable test with high sensitivity/specificity to predict or confirm the diagnosis of such cases in routinely clinical practice. In the present study, we aimed to find any potential correlation between serum levels of GGT and CRP- Q and/or hs- CRP and WBC count as the well- known markers of inflammation in human body.
Materials and Methods: Through a cross- sectional study, serum levels of CRP-Q, hs- CRP, and GGT, in addition to WBC count were measured in 1,500 healthy people, referred to Blood Transfusion Organization from across the country. Data were analyzed after the patients were checked by physical examination for ruling out the presence of any inflammation or other illness. Serum levels of GGT, CRP- Q, hs- CRP, and WBC count were analyzed by SPSS for windows version 16.
Results: Analysis showed a positive linear correlation between CRP- Q and hs- CRP with GGT also confirmed by non- parametric tests.
Conclusion: Serum GGT may be an inflammatory index and a useful marker in approaching to inflammatory diseases.
https://ijp.iranpath.org/article_8337_c1aa4f155247820356d9334e9270e7bf.pdf
2012-09-01
209
214
Gamma Glutamyltransferase
C- Reactive Protein
White Blood Cell Count
Alireza
Abdollahi
dr_p_abdollahi@yahoo.com
1
Dept. of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Saeed
Shoar
2
Development Association for Clinical Study (DACS), Student Scientific Research Center (SSCR), Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Oda E, Kawai R, Watanabe K, Sukumaran V. Prevalence of Metabolic Syndrome Increases with the Increase in Blood Levels of Gamma Glutamyltransferase and Alanine Aminotransferase in Japanese Men and Women. Inter Med 2009; 48: 1343-1350.
1
Lee DS, Evans JC, Robins SJ, et al. Gamma glutamyl transferase and metabolic syndrome, cardiovascular disease, and mortality risk: the Framingham Heart Study. Arterioscler Thromb Vasc Biol 2007; 27: 127-133.
2
Misra A. C- Reactive Protein in Young Individuals: Problems and Implications for Asian Indians. Nutrition 2004;20:478–81.
3
Kim JH, Lee HR, Han AR, Im JA, Lee DC. Relationship between Serum gamma-glutamyltransferase Level and Serum Ferritin Level in Healthy Adults. J Korean Acad Fam Med 2006;27(8):645-51.
4
Schulman JD, Goodman SI, Mace JW, Patrick AD, Tietze F, Butler EJ. Glutathionuria: inborn error of metabolism due to tissue deficiency of gamma-glutamyl transpeptidase. Biochem. Biophys Res Commun 1975;65(1):68–74.
5
Yokoyama H. Gamma glutamyl transpeptidase (gammaGTP) in the era of metabolic syndrome in Japanese. Nihon Arukoru Yakubutsu Igakkai Zasshi 2007;42 (3):110–24.
6
Fraser A, Thinggaard M, Christensen K, Lawlor DA. Alanine aminotransferase, gamma-glutamyltransferase (GGT) and all-cause mortality: results from a population-based Danish twins study alanine aminotransferase, GGT and mortality in elderly twins. Liver Int 2009;29(10):1494-9.
7
Lee DH, Blomhoff R, Jacobs DR JR. Is serum gamma glutamyltransferase a marker of oxidative stress? Free Radic Res 2004;38: 535–9.
8
Raulf M, Stüning M, König W. Metabolism of leukotrienes by L-gamma-glutamyl-transpeptidase and dipeptidase from human polymorphonuclear granulocytes. Immunology 1985;55(1):135–47.
9
Fraser A, Harris R, Sattar N, Ebrahim S, Smith GD, Lawlor DA.Gamma-glutamyltransferase is associated with incident vascular events independently of alcohol intake: analysis of the British women’s heart and health study and meta-analysis. Arterioscler Thromb Vasc Biol 2007;27:2729–35.
10
Lee DH, Jacobs DR JR.Serum gamma-glutamyltransferase: new insights about an old enzyme. J Epidemiol Community Health 2009;63(11):884-6.
11
Targher G, Kendrick J, Smits G, Chonchol M. Relationship between serum gamma-glutamyltransferase and chronic kidney disease in the United States adult population. Findings from the National Health and Nutrition Examination Survey 2001-2006. Nutr Metab Cardiovasc Dis 2010;20(8):583-90.
12
Strasak AM, Rapp K, Brant LJ, Hilbe W, Gregory M, Oberaigner W, et al. Association of gamma- glutamyltransferase and risk of cancer incidence in men: a prospective study. Cancer Res 2008;68:3970–7.
13
Lee DH, Silventoinen K, Hu G, Jacobs DR, Jousilahti P, Sundvall J, et al. Serum gamma glutamyltransferase predicts nonfatal myocardial infarction and fatal coronary heart disease among 28,838 middleaged men and women. Eur Heart J 2006;27:2170–6.
14
Ryu S, Chang Y, Kim DI, Kim WS, Suh BS. gamma-Glutamyltransferase as a predictor of chronic kidney disease in nonhypertensive and nondiabetic Korean men. Clin Chem 2007;53:71–7.
15
Lee DH, Jacobs DR Jr, Gross M, Steffes M. Gamma glutamyltransferase is a predictor of incident diabetes and hypertension: the Coronary Artery Risk Development in Young Adults (CARDIA) Study. Clin Chem 2003;49:1358–66.
16
Lee DH, Steffen LM, Jacobs DR Jr. Association between serum gamma glutamyltransferase and dietary factors: the Coronary Artery Risk Development in Young Adults (CARDIA) Study. Am J Clin Nutr 2004;79:600–5.
17
Westerbacka J, Corner A, Tiikkainen M, Tamminen M, Vehkavaara S, Häkkinen AM, et al. Women and men have similar amounts of liver and intra-abdominal fat, despite more subcutaneous fat in women: implications for sex differences in markers of cardiovascular risk. Diabetologia 2004;47:1360–9.
18
Lee DH, Steffes M, Jacobs D. Can persistent organic pollutants explain the association between serum gamma-glutamyltransferase and type 2 diabetes? Diabetologia 2008;51: 402–7.
19
Miyatake N, Matsumoto S, MakinoH, Numata T. Comparison of hepatic enzymes between Japanese men with and without metabolic syndrome. Acta Med Okayama 2007; 61:31–4.
20
Fraser A, Harris R, Sattar N, Ebrahim S, Davey Smith G, Lawlor DA. Alanine aminotransferase, gamma glutamyltransferase and incident diabetes: the British Women’s Heart and Health Study and meta-analysis. Diabetes Care 2009;32:741–50.
21
Whitfield JB. Gamma glutamyl transferase. Crit Rev Clin Lab Sci 2001;38:263–355.
22
Emdin M, Pompella A, Paolicchi A. Gamma- glutamyltransferase, atherosclerosis, and cardiovascular disease: Triggering oxidative stress within the plaque. Circulation 2005;112:2078–80.
23
Paolicchi A, Emdin M, Ghliozeni E, Ciancia E, Passino C, Popoff G, et al. Atherosclerotic plaques contain gamma-glutamyl transpeptidase activity. Circulation 2004;109:1140.
24
Bo S, Gambino R, Durazzo M, Guidi S, Tiozzo E, Ghione F, et al. Associations between gamma-glutamyl transferase,metabolic abnormalities and inflammation in healthy subjects from a population-based cohort: A possible implication for oxidative stress. World J Gastroenterol 2005;11:7109–17.
25
Ulus T, Yildirir A, Demirtas S, Demir O, Sade LE, Bozbas H, et al. Serum gamma-glutamyl transferase activity: A new marker for stent restenosis? Atherosclerosis 2007;195:348–53.
26
Strasak AM, Kelleher CC, Klenk J, Brant LJ, Ruttmann E, Rapp K, et al. Longitudinal change in serum gamma-glutamyltransferase and cardiovascular disease mortality: a prospective population-based study in 76,113 Austrian adults. Arterioscler Thromb Vasc Biol 2008;28(10):1857-65.
27
Lee YJ, Kim JK, Lee JH, Lee HR, Kang DR, Shim JY. Association of serum gamma-glutamyltransferase with C-reactive protein levels and white blood cell count in Korean adults. Clin Chem Lab Med 2008; 46(10):1410-5.
28
Oda E, Kawai R. Comparison between high-sensitivity C- reactive protein (hs- CRP) and white blood cell count (WBC) as an inflammatory component of metabolic syndrome in Japanese. Intern Med 2010;49(2):117-24.
29
Lee MY, Koh SB, Koh JH, Nam SM, Shin JY, Shin YG, et al. Relationship between gamma- glutamyltransferase and metabolic syndrome in a Korean population. Diabet Med 2008;25(4):469-75.
30
ORIGINAL_ARTICLE
Immunohistochemical Expression of p53 and bcl2 in Colorectal Adenomas and Carcinomas Using Automated Cellular Imaging System
Background & Objective: The current approaches to reduce the risk of colorectal carcinoma are through the detection and removal of the precursor lesion” adenomatous polyps”. The study was conducted to evaluate the immunohistochemical expression of p53 and bcl2 in colorectal adenomas and carcinomas.
Patients and Methods: A total of 86 cases, 33 colorectal adenomas, 33 colorectal adenocarcinomas and 20 samples of non -tumerous colonic tissue as control, were included in this retrospective study. Sections were stained immunohistochemically for p53 and bcl2. Scoring was performed using Digimizer software. Data were analyzed using SPSS program (Statistical Package for Social Sciences) version 16 and Microsoft Office Excel 2007.
Results: The frequency of p53 positive cases was significantly higher in carcinoma than adenoma while the frequency of bcl2 positive cases was significantly higher in adenoma than carcinoma. P53 expression was significantly higher in large sized adenomas, villous configuration, severe dysplasia, and multiple lesions. Bcl2 expression showed significantly correlated with adenomas of small size, solitary, tubular, and mild dysplasia. There was a significant correlation between bcl2 expression and non-mucinous carcinoma and a negative correlation with tumor size.Therewas an inverse relationship between bcl2 and p53 expression in both colorectal adenomas and carcinomas.
https://ijp.iranpath.org/article_8338_525b1c3a1c7e0539160f3e216fd42fc4.pdf
2012-09-01
215
223
P53 Antigen
c-bcl-2 Proto-Oncogene
Adenoma
Colorectal carcinomas
Ban
Qasim
dr.banqasim@yahoo.com
1
Dept. of Pathology and Forensic Medicine, Al-Nahrain University, Baghdad, Iraq
LEAD_AUTHOR
Husam
Ali
2
Dept. of Pathology and Forensic Medicine, Al-Nahrain University, Baghdad, Iraq
AUTHOR
Alaa
Hussein
alaa_ghani@yahoo.com
3
Dept. of Pathology and Forensic Medicine, Al-Nahrain University, Baghdad, Iraq
AUTHOR
Takahashi M, Wakabayashi K. Gene mutations and altered gene expression in azoxymethane-induced colon carcinogenesis in rodents. Cancer Sci 2004;95(6):475-80.
1
Calvert PM , Frucht H. The genetics of colorectal cancer. Ann Intern Med 2002;137(7):603-12.
2
Morán A, Ortega P, de Juan C, Fernández-Marcelo T, Frías C, Sánchez-Pernaute A, et al. Differential colorectal carcinogenesis: molecular basis and clinical relevance. World J Gastrointest Oncol 2010;2(3):151-8.
3
Ozaki T, Nakagawara A. p53: the attractive tumor suppressor in the cancer research field. J Biomed Biotechnol 2011;2011:603925.
4
Menezes HL, Jucá MJ, Gomes EG, Nunes BL, Costa HO, Matos D. Analysis of the immunohistochemical expressions of p53, bcl-2 and Ki-67 in colorectal adenocarcinoma and their correlations with the prognostic factors. Arq Gastroenterol 2010;47(2):141-7.
5
Zavrides H, Zizi-Sermpetzoglou A, Elemenoglou I, Papatheofanis I, Peros G, Athanasas G, et al. Immunohistochemical expression of bcl-2 in UICC stage I and III colorectal carcinoma patients: correlation with c-erbB-2, p53, ki-67, CD44, laminin and collagen IV in evaluating prognostic significance. Pol J Pathol 2006;57(3):149-59.
6
Saneei MH, Razi M. Role of bcl2 oncoprotein in carcinogenesis of colorectal neoplasia and its correlation with histopathologic and prognostic parameter. Journal of Isfahan Medical School 2005;23(78):30-6.
7
Yan-fang A, Yong M, Jing-hua L. The expressions of PCNA and Bcl-2 in colorectal adenoma and carcinoma and their clinicopathological and prognostic significance. Acta Academiae Medicinae Xuzhou 2006;6:11-7.
8
Contu PC, Contu SS, Moreira LF. Bcl-2 expression in rectal cancer. Arq Gastroenterol 2006; 43(4):284-7.
9
Ishii T, Notohara K, Umapathy A, Mallitt KA, Chikuba H, Moritani Y, et al. Tubular adenomas with minor villous changes show molecular features characteristic of tubulovillous adenomas. Am J Surg Pathol 2011;35(2):212-20.
10
Sheikh RA, Min BH, Yasmeen S, Teplitz R, Tesluk H, Ruebner BH, et al. Correlation of Ki-67, p53, and Adnab-9 immunohistochemical staining and ploidy with clinical and histopathologic features of severely dysplastic colorectal adenomas. Dig Dis Sci 2003;48(1):223-9.
11
Tzouvala M, Lazaris AC, Papatheodoridis GV, Kouvidou C, Papathomas TG, Kavantzas N, et al. Potential role of apoptosis and apoptotic regulatory proteins in colorectal neoplasia: correlations with clinico-pathological parameters and survival. Dig Dis Sci 2008;53(2):451-60.
12
Vernillo R, Lorenzi B, Banducci T, Minacci C, Vindigni C, Lucenti Fei A , et al. Immunohistochemical expression of p53 and Ki67 in colorectal adenomas and prediction of malignancy and development of new polyps. Int J Biol Markers 2008;23(2):89-95.
13
Einspahr JG, Martinez ME, Jiang R, Hsu CH, Rashid A, Bhattacharrya AK, et al. Associations of Ki-ras proto-oncogene mutation and p53 gene overexpression in sporadic colorectal adenomas with demographic and clinicopathologic characteristics. Cancer Epidemiol Biomarkers Prev 2006;15(8):1443-50.
14
Nussrat FL, Ali HH, Hussein HG,AL-Ukashi RJ. Immunohistochemical expression of ki-67 and p53 in colorectal adenomas: a clinicopathological study. Oman Med J 2011;26(4):229-234.
15
Abdulamir AS, Hafidh R, Mahdi L, Al-jeboori L, Abubaker F, Abbas KA. The interplay between p53 and p21 tumor suppressor proteins in the transformation of colorectal adenoma to carcinoma. Am J Immunol 2008;4:14-22.
16
Mulder JW, Wielenga VJ, Polak MM, van den Berg FM, Adolf GR, Herrlich P, et al. Expression of mutant p53 protein and CD44 variant proteins in colorectal tumorigenesis. Gut 1995;36(1):76-80.
17
Gurzu S, Jung J, Mezei T, Pavai Z. The correlation between the immunostains for p53 and Ki67 with bcl-2 expression and classical prognostic factors in colorectal carcinomas. Rom J Morphol Embryol 2007;48(2):95-9.
18
Ghavam-Nasiri MR, Rezaei E, Ghafarzadegan K, Seilanian-Toosi M, Malekifard H. Expression of p53 in colorectal carcinoma: correlation with clinicopathologic features. Arch Iranian Med 2007;10(1):38-42.
19
Nancy Y, Assad MD, Mona A. Prognostic value of Cyclin D1 and p53 protein in colorectal carcinoma. Cancer Institute 2000;12:283-92.
20
Soong R, Powell B, Elsaleh H, Gnanasampanthan G, Smith DR, Goh HS, et al. Prognostic significance of TP53 gene mutation in 995 cases of colorectal carcinoma. Influence of tumor site, stage, adjuvant chemotherapy, and type of mutation. Eur J Cancer 2000;36(16):2053-60.
21
Gervaz P, Bouzourene H, Cerottini JP, Chaubert P, Benhattar J, Secic M, et al. Dukes B colorectal cancer: distinct genetic categories and clinical outcome based on proximal or distal tumor location. Dis Colon Rectum 2001;44(3):372-3.
22
Lanza G Jr, Maestri I, Dubini A, Gafa R, Santini A, Ferretti S, et al. P53 expression in colorectal cancer: relation to tumor type, DNA ploidy pattern, and shortterm survival. Am J Clin Pathol 1996;105(5):604-12.
23
Garrity MM, Burgart LJ, Mahoney MR, Windschitl HE, Salim M, Wiesenfeld M, et al. Prognostic value of proliferation, apoptosis, defective DNA mismatch repair, and p53 overexpression in patients with resected Dukes' B2 or C colon cancer: a North Central Cancer Treatment Group Study. J Clin Oncol 2004;22(9):1572-82.
24
Sinicrope FA, Ruan SB, Cleary KR, Stephens LC, Lee JJ, Levin B. bcl-2 and p53 oncoprotein expression during colorectal tumorigenesis. Cancer Res 1995;55(2):237-41.
25
Saleh HA, Jackson H, Banerjee M. Immunohistochemical expression of bcl-2 and p53 oncoproteins: correlation with Ki67 proliferation index and prognostic histopathologic parameters in colorectal neoplasia. Appl Immunohistochem Mol Morphol 2000;8(3):175-82.
26
Yang HB, Chow NH, Sheu BS, Chan SH, Chien CH, Su IJ. The role of bcl-2 in the progression of the colorectal adenoma-carcinoma sequence. Anticancer Res 1999;19(1B):727-30.
27
Hussain AG. bcl2 overexpression in colorectal carcinoma. Iraqi J Med Sci 2008;6:66-76.
28
Demirbaş S, Sücüllü I, Yildirim S, Celenk T. Influence of the c-erb B-2, nm23, bcl-2 and p53 protein markers on colorectal cancer. Turk J Gastroenterol 2006;17(1):13-9.
29
ORIGINAL_ARTICLE
Chemotherapy and Management of Locally Advanced Carcinoma Cervix
Background & Objective:the present study was undertaken to ascertain the incidence of early, advanced cancer cervix and its recurrence and role of chemotherapy in locally advanced cancer cervix.
Material and Methods: The present study was conducted in the Departments of Obstetrics & Gynaecology, Pathology and Radiotherapy and the specimens examined in the Department of Pathology of Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India from July 2009 to July 2010.One hundred histologically confirmed carcinoma cervix cases were divided into two groups. I: Study group of cases receiving different types of chemotherapy and II: Control group comprised of cases on pre and postoperative radiotherapy and surgery. Patients were staged and locally advanced inoperable cases (Stage IB-IVA) were given different types of chemotherapy. Thereafter subjective and objective assessment was made according to World Health Organization (WHO) criteria of tumor response.
Results: Majority of cases (60.0%) presented in stage IIB of disease by International Federation of Gynecology and Obstetrics (FIGO) classification. Commonest symptom was postmenopausal vaginal bleeding, 70.0% cases. 68.0% & 56.0% cases showed complete response (CR) on chemotherapy and radiotherapy respectively. Stage IIB patients showed the best response with CR in 70.0% & partial response (PR) in 16.7% cases. Colposcopic evaluation on 12 -18 months follow up showed 08 to be disease free, with decrease in size of tumor.
Conclusion: Chemotherapy is an effective mode of therapy and can be considered as an adjunct to surgery or radiotherapy to improve the overall survival of cancer cervix patients.
https://ijp.iranpath.org/article_8339_1b6fa96fff86e65aa66c517af04b3cbd.pdf
2012-09-01
224
230
Cervix Cancer
chemotherapy
Rajyashri
Sharma
1
Department of Gynaecology, Jawaharlal Nehru Medical College, Aligarh Muslim University,Aligarh (UP), India
AUTHOR
Huma
Firdaus
2
Department of Gynaecology, Jawaharlal Nehru Medical College, Aligarh Muslim University,Aligarh (UP), India
AUTHOR
Veena
Maheshwari
3
Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh (UP), India
AUTHOR
Kafil
Akhtar
drkafilakhtar@gmail.com
4
Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh (UP), India
LEAD_AUTHOR
Shahid Ali
Siddiqui
5
Department of Radiotherapy, Jawaharlal Nehru Medical College, Aligarh Muslim University , Aligarh (UP), India
AUTHOR
National Comprehensive Cancer Network. NCCN Clinical Practice Guidelines in Oncology: Cervical Cancer v.1.2009. Available at http: //www.nccn.org/professionals/physician_gls/PDF/cervical.pdf. Accessed August 25, 2009.
1
Solomon D, Breen N, McNeel T. Cervical cancer screening rates in the United States and the potential impact of implementation of screening guidelines. CA Cancer J Clin 2007;57 (2):105-11.
2
Smith RA, Cokkinides V, Brawley OW. Cancer screening in the United States, 2009: a review of current American Cancer Society guidelines and issues in cancer screening. CA Cancer J Clin2009;59(1):27-41.
3
Markman M. Presentation of chemotherapy options for cervix cancer on cancer-related Internet sites. J Womens Health (Larchmt) 2009;18(6):827-9.
4
Kumar JV, Doval DC, Rao R, Rawal S. A retrospective study of patients with locally advanced cancer of the cervix treated with neoadjuvant chemotherapy followed by radical surgery. Int J Gynecol Cancer 2009;19(3):417-22.
5
Zhao FH, Lin MJ, Chen F, Zhang R, Belinson JL, Sellors JW, et al.. Primary screening for cervical cancer: a pooled analysis of individual patient data from 17 population-based studies from China. Lancet Oncol 2010;11(12):1160-71.
6
Gonzalez DA, Zarba JJ, Patel F, Alcedo JC, Beslija S, Casanova L, et al.. Phase III, Open-Label, Randomized Study Comparing Concurrent Gemcitabine Plus Cisplatin and Radiation Followed by Adjuvant Gemcitabine and Cisplatin Versus Concurrent Cisplatin and Radiation in Patients With Stage IIB to IVA Carcinoma of the Cervix. J Clin Oncol2011;29(13):1678-85.
7
World Health Organization. WHO/ICO Information Centre on Human Papilloma Virus (HPV) and Cervical Cancer. Available at http://www.who.int/hpvcentre/statistics/en/.. Accessed April 2, 2011.
8
Duenas-Gonzalez A, Lopez-Graniel C, Rivera L, Guadarrama R, Chanona G, De La Garza J. Clinicopathological Variables Predictive of Clinical Outcome in Patients with Cervical Cancer Treated with Cisplatin-based Neoadjuvant Chemotherapy Followed by Radical Hysterectomy. Anticancer Res 2010;1:201-8.
9
Panici PB, Stefano G, Alessandro C, Mariangela A. Pattern of Failures and Clinical Outcome of Patients with Locally Advanced Cervical Cancer Treated with a Tailored Integrated Therapeutic Approach. Anticancer Res 2010;1:3731-5.
10
Panici PB, Stefano G, Alessandro C, Mariangela A, Costantino M, Fabio L. Neoadjuvant Chemotherapy and Radical Surgery Versus Exclusive Radiotherapy in Locally Advanced Squamous Cell Cervical Cancer: Results From the Italian Multicenter Randomized Study. J Clin Oncol 2002;20(1):179-88.
11
ORIGINAL_ARTICLE
Immunofluorescence Pattern of Autoimmune Bullous Diseases in Iranian Patients
Background & Objective: Autoimmune bullous diseases are associated with autoimmunity against structural components in the skin and mucous membranes. Autoantibodies are against the intercellular junctions in pemphigus disease and hemidesmosomal unchoring complex in pemphigiod diseases and epidermolysis bullosa aquisita. The tissue-bound and circulating serum autoantibodies can be detected with direct immunofluorescence (DIF) and indirect Immunofluorescence (IIF) tests. The aim of this study was to pinpoint the immunofluorescence pattern of Iranian patients with autoimmune bullous diseases.
Methods: In a prospective case series study, sixteen patients with autoimmune disease enrolled in the study for two years. Perilesional skins and sera from the patients were used in DIF and IIF for detection of immunofluorescence pattern.
Results:Out of 16 cases, 9 cases had pemphigus and 7 cases had bullous pemphigoid. All cases of pemphigus had positive DIF in intercellular region with lacelike pattern; IgG was detected in all cases, IgA in 1(11.1%) case and C3 in 3 (33.3%) cases. One (11.1%) case of pemphigus had positive IIF in intercellular region with lacelike pattern; circulating autoantibodies were IgG and IgA. All cases of bullous pemphigiod had positive DIF in dermal-epidermal Junction with linear pattern. IgG was detected in all cases, IgM in one (14.3%) case, and C3 in six (85.7%) cases. One (14.3%) case of bullous pemphoid had positive IIF in dermal-epidermal Junction with linear pattern; circulating autoantibody was IgG.
Conclusion: Immunofluorescence tests are sensitive diagnostic methods for autoimmune bullous diseases. IIF positive cases in our study were lower compared to the previous reports.
https://ijp.iranpath.org/article_8340_637b9478edd93bb7a37797e8983dbcdf.pdf
2012-09-01
231
235
Immunofluorescence
Bullous Skin Diseases
Iran
Alireza
Monsef
monsef_ar2001@yahoo.com
1
Dept. of Pathology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran
LEAD_AUTHOR
Mahmood
Farshchian
2
Dept. of Dermatology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran
AUTHOR
Mohammad
Jafari
3
Dept. of Pathology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran
AUTHOR
Mehdi
Farshchian
4
Dept. of Dermatology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran
AUTHOR
Mihai S, Sitaru C. Immunopathology and molecular diagnosis of autoimmune bullous diseases. J Cell Mol Med 2007;11(3):462-81.
1
Razzaque AA. Diagnosis of bullous disease and studies in the pathogenesis of blister formation using immunopathological techniques. J Cuetan Pathol 1989;11(4): 237-48.
2
Elder DE, Elenitsas R, Johnson BL, Murphy GF. Lever's Histopathology of the Skin. 9th ed. Philadelphia:Lippincott Williams and Wilkins;2005.
3
Henry JB. Clinical Diagnosis and Management by Laboratory Methods; 21th ed. Philadelphia Elsevier;2007.
4
Fine JD, Neises GR, Katz SI. Immunofluresence and Immunoelectron Microscopic studies in cicartrical pemphigoid. J Invest Dermatol 1984;82(1):39-43.
5
Rosai J, Ackerman LV. Ackerman Surgical Pathology. 9th ed. New York:Mosby; 2004.
6
Kumar V, Abbas AK, Nelson F. Pathologic Basis of Disease. 7th ed. Philadelphia: Elsevier;2005.
7
Tan T, Ratnam KV. Immunoflurescence studies of pemphigus and pemphigoid in Singapore. Ann Acad Med Singapore 1983;12(1):13-8.
8
Weigand DA, Clements MK. Direct immunofluorence in bullous pemphigoid: effects of extent and location of lesions. J Am Acad Dermatol 1989;20(3):437-40.
9
Kirtsching G, Wojnarowska F. IgA basement membrane zone autoantibodies in bullosa pemphioid detect epidermal antigens of 270- 280 K Da, 230 K Da, and 180 K Da molecular weight by immunoblotting. Clin Exp Dermatol 1999; 24(4): 302-7.
10
ORIGINAL_ARTICLE
Effect of Low Level Laser Therapy on the Surgical Wound Healing in Hamster
Background and Objectives: Accelerating wound healing is a very important factor for patients to return to ordinary life. Laser seems to have positive effect on cutaneous wound healing. This study aimed to investigate the effect of red light laser 630 nm on cutaneous surgical wound in hamster and to compare outcomes such as angiogenesis, number of fibroblasts and collagen formation in intervention and control group.
Materials and Methods: This experimental study carried out with collaboration of Qazvin University of Medical Sciences with Mehregan Pet Clinic in winter of 2009. Thirty suri hamsters were randomly allocated in two groups and four parallel wounds were made on their backs. The intervention group was radiated with red-light laser 630 nm. After 2,5,10 and 14 days, skin biopsy were obtained and number of blood vessels, fibroblast and collagen production were compared with the control group. Data were analyzed using independent sample student t test using SPSS version 10 and P value less than 0.05 was considered significant.
Result: Evaluation of wounds in experimental and control groups showed significantly increased number of vessels and fibroblasts as well as collagen production in laser radiating group (P<0.05).
Conclusion: Laser therapy (630 nm) can accelerate wound healing in comparison with control group.
https://ijp.iranpath.org/article_8341_10bf82fcde523d5f67fc2cd126cf5fec.pdf
2012-09-01
2236
240
Laser
Wound Healings
Hamster
Neda
Nasirian
drsnasirianqums@gmail.com
1
Dept. of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran
LEAD_AUTHOR
Ali
Nasirian
2
Dept. of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran
AUTHOR
Houreld N, Abrahamse H. Low-level laser therapy for diabetic wound healing. Source: The diabetic foot, Date: 12/22/2005, Available online AT: http://www.mylightwave.com/docs/HighBeamResearch%20Low-level%20laser%20therapy%20for%20diabetic%20foot%20wound%20healing._Wound%20care_.pdf. Last accessed 22 Dec 2011.
1
Bolton P, Dyson M, Young S. The effect of polarized light on the release of growth factors from the U-937 macrophage – Like cell line. Laser Ther 1992;4(1):33-8.
2
Dyson M, Young S. Effect of Laser Therapy on Wound Contraction and Cellularity in Mice. Lasers Med Sci 1986;1(2):125-30.
3
Kumar V, Abbas AK, Fausto N. Robbins and Cotran Pathologic Basis of Diseases. 8th ed. Saunders:New York;2007.
4
Walsh LJ. The current status of low level laser therapy in dentistry. Part 1. Soft tissue applications. Aust Dent J 1997;42(4): 247-54.
5
Tunér J, Hode L. laser therapy: Clinical practice and scientific background. 1st Sweden:Prima books;2002.
6
Pinheiro AL, Carneiro NS, Vieira, AL, Brugnera A Jr, Zanin FA, Barros RA, et al. Effects of low level laser therapy on malignant cells in vitro study. J Clin Laser Med Surg 2002;20(1):23-6.
7
Ohshiro T, Calderhead RG. Progress in low-level laser therapy. John Wiley &Sons: England;1991.
8
Steinlechner CWB, Dyson M. The effects of low level laser therapy on the proliferation of keratinocytes. Laser Ther 1993;5:65-73.
9
Loeveschall H, Arenbolt-Bindslev D. Effect of low-level diode laser irradiation of human oral mucosa fibroblasts in vitro. Laser Surg Med 1994; 14(4):347-54.
10
Reddy GK, stehnoBittel L, Enwemeka CS. Laser photostimulation accelerates wound healing in diabetic rats. Wound Repair Regen 2001; 9(3):248-55.
11
YU W, Naim JO, lanzafame RJ. Effects of photostimulation on wound healing in diabetic mice. Laser Surg Med 1997;20(1):59-63.
12
Matić M, Lazetić B, Poljacki M, Duran V, Ivkov-Simić M. Low-level laser irradiation and its effect on repair processes in the skin. Med Pregl 2003; 56(3-4):137-41.
13
14-HamblinMR.Mechanism of low-level light therapy. Available online at www.mgh.harvard.edu/wellman/people/mhamblin.asp
14
ORIGINAL_ARTICLE
Frequency of Nosocomial Infections with Antibiotic Resistant Strains of Acinetobacterspp. in ICU Patient
Background and Objective:Acinetobacter spp.a Gram-negative coccobacillus is increasingly reported as important cause of nosocomial infections. Multi-drug resistance (MDR) ofAcinetobacterspp., raises concerns over our ability to treat serious infections with these organisms. The aim of this study was to determine the frequency and associated risk factors for infections with MDR Acinetobacter spp. in ICU patients ofShahid Mostafa Khomeini Hospital, Tehran, Iran.
Patients and Methods:This descriptive-analytical andcross-sectional study was designed in 3 years period from April 2008 to March 2010 on 130 patients. For bacteriological analysis, sputum, blood, urine and wound specimens were used from patients within >48 hr after admission. Patient’s clinical and epidemiologic data were collected, from recordedfile, and correlated to Acinetobacter spp. infection. The data were analyzed using SPSS16 statistical software,chi-square, and Mann-Whitney test.
Results: The frequency of Acinetobacterspp. infection separately by years was 21.5%, 30.8% and 47.7% in 2008, 2009, 2010, respectively. All isolateswere resistant to carbnicillin, piperacillin, cefotaxime and cephalotin, 99.2% to ciprofloxacin, cotrimoxazole and chloramphenicol, 97.7% to imipenem, 95.4% to tetracycline and 91.5% to gentamicin. The highest sensitivity was to amikacin 14.6%.
Conclusion:Nosocomialinfections with Acinetobacter spp. during the three years, was a growing trend, and all isolates were MDR and highest susceptibility was to Amikacin. Most important risk factors wereincorrect diagnosis, inappropriate usage, doses, andtime of antibiotic therapy, inappropriate formulation of some antibiotics.
https://ijp.iranpath.org/article_8342_373204eb810f06f84b84322f4bd8104a.pdf
2012-09-01
241
245
AcinetobacterInfection
Multidrug resistance
Intensive care unit
Maryam
Amini
mmamini55602@gmail.com
1
Dept. of Infectious Diseases and Tropical Medicine, Shahed University, Tehran, Iran
LEAD_AUTHOR
Ali
Davati
2
Dept. of Social Medicine, Shahed University, Tehran, Iran
AUTHOR
Mahdieh
Golestanifard
3
Dept. of Infectious Diseases and Tropical Medicine, Shahed University, Tehran, Iran
AUTHOR
Akbari M, Davoudzade M, Rozbahani H, Tarhi M, Bayat A, Radsari E. Types of pathogens isolated from hospital staff gowns Martyrs Khorramabad tribes in 2003-2004 years. Lorestan University of Medical Sciences Journal 2005;7(2):11-6.
1
Khosroshahi N, Sharifi M. Carbapenem resistant Acinetobacter strains isolated from patients in intensive care units and equipment of health centers in Qazvin in 2006-2007. Iranian Journal of Medical Microbiology 2007;3(1):33-8.
2
Acintobacter. Available at: http:// www.infectious disease. Louisiana.dhh.gov. Accessed 2008.
3
Bennani B, Selmani R, MahmoudM, Nejjari C, KanjaaN.Nosocomial pneumonia in mechanically ventilated patients: prospective study in intensive care unit of Fez university hospital. Saudi journal of Anaesthesia 2008;2(2):46-51.
4
WarehamD, BeanD, KhannaP, Hennessy E, Krahe D, Ely A, et al. Blood streaminfection due to Acintobacterspp: epidemiology, risk factor and impact of multi drug resistance. Eur J Clin Microbiol Infect Dis 2008;27(7):607-12.
5
Munoz-price LS, Weinstein RO. Acintobacter infection. N Engl J Med. 2008; 358(12):1271-81.
6
Mshana ST, Kamugisha ER, MiramboMA, Chakraborty TR, Lyamuya EL. Prevalenceof multi resistant gram negative organisms in a tertiary hospital in Mwanza,Tanzania. BMC Res Notes 2009; 2: 49-54.
7
Zarrilli RA, Crispino MA, BagattiniMA, Barretta EL, Di Popolo AN,Triassi MA, et al. Molecular epidemiology ofsequentialout breaks of A.baumanniiinICU shows theemergence ofcarbapenemresistance. J Clin Microbial 2004;42(3):946-53.
8
Patwardhan R, Dhakephalkar P, Niphadkar K, Chopade B. A Study ofnosocomialpathogenin ICU withspecialreference to multi resistant Acintobacterbaumanniiharbouringmultiple plasmids. Indian J Med Res 2008;128(2):178-87.
9
Jen shih MU, Yao lee NA, Chun lee HS, Ming chang CH, Jung wu CH, Ling chen PO, et al. Risk factor ofmulti drug resistance in nosocomialbacteremia due to Acinetobacter baumannii. J Microbiol ImmunolInfect 2008;41(2):118-23.
10
SunenshineRE, Wright MA, MaragakisLI, Harris AN, Song XI, HebdenJO, et al. Multi drug resistant Acintobacterinfection mortality rateandlength ofhospitalization. Emerg Infect Dis 2007; 13(1):97-103.
11
Ghorbanalizadegan M, Ranjbar R, Izadi M, Esmaili D, Ahmadi A, Goudarzi Z. Prevalence of Pseudomonas aeruginosa and Acinetobacter with multi drug resistance in patients admitted to hospital Baghiatollah. Ilam university of Medical Sciences Journal 2007;15(1):1-5.
12
Flanders S, Collard H, Saint S. Nosocomial pneumonia: state of the science. Am J Infect Control 2006;34(2):84-93.
13
14. Van Looveren M, Goossens H.Antimicrobial resistance of Acinetobacter spp. in Europe. Clin Microbiol Infect 2004;10(8):684–704.
14
Sadeghifard N, Ranjbar R,Ghasemi A, Pakzad I, Zaimi J,Zameri A, et al. Evaluation of drug resistant strains of Acinetobacter baumannii and other species isolated from three hospitals, Tehran. Ilam university of Medical Sciences Journal 2006;14(3):29-36.
15
Basustaoglu AC, Kisa O, Sacilik SC. Epidemiological characterization of hospital-acquired Acinetobacter baumannii isolates from a 1500-bed teaching hospital by phenotypic and genotypic methods. J Hospital Infect 2001;47(3):246-49.
16
Khaltabadi R, Moniri R, Shajari G, Nazem M,Mousavi G, Ghasemi A,et al.The pattern of antibiotic resistance and spread of antibiotic resistance genes in Acinetobacter strains isolated from Kashan. Faiz J Med Res 2009;12(4):60-6.
17
Hujer KM, Hujer AM, Hulten EA, Bajaksouzian S, Adams JM, Donskey CJ, et al. Analysis of antibiotic resistance genes in multi drug resistant Acintobacter spp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. Antimicrob Agent Chemother 2006;50(12): 4114-23.
18
ORIGINAL_ARTICLE
Inguinal Lymph Node Metastasis as the First Presentation of Endometrial Carcinoma
Endometrial adenocarcinoma is uncommon in fewer than 40-year-old people. The first common sign and symptoms are abnormal vaginal bleeding and discharge. Metastasis is occurred in the late stage. Here we present a 31-year-old nullipar woman who was admitted to surgical ward with enlarged right inguinal lymphnode from one month ago. She had no history of previous malignancy, infectious condition, vaginal bleeding, and discharge. Abdominal sonography revealed no abnormality. She underwent surgical biopsy and metastatic adenocarcinoma most suspected from genital tract was reported. Based on pathological recommendation diagnostic curettage was performed and endometrial adenocarcinoma of conventional type as a first origin was confirmed. To our knowledge metastasis to inguinal lymph node as a first manifestation of endometrial carcinoma is rare.
https://ijp.iranpath.org/article_8343_1fa58a26cd251126e5ae2c6ce29b0b54.pdf
2012-09-01
246
250
Endometrial Carcinoma
Groin
Lymph node
Metastasis
Shokouh
aghipour zahir
shokouh_ zahir@yahoo.com
1
Dept. of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
LEAD_AUTHOR
Kiyanoosh
Sedaghat
2
Dept. of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
AUTHOR
Saeed
Kargar
3
Dept. of Surgery, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
AUTHOR
Di Cristofano A, Ellenson LH. Endometrial carcinoma. Annu Rev Pathol 2007;2:57-85.
1
Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, et al. Cancer statistics. 2008. CA Cancer J Clin 2008;58(2):71-96.
2
Abeloff MD, Armitage JO, Niederhuber JE, Kastan MB, McKenna WG. Abeloff’s Clinical Oncology. 4th ed. Philadelphia:Churchill Livingstone;2008.
3
Scholz HS, Lax S, Petru E, Benedicic C, Winter R. Inguinal lymph node metastasis as the presenting symptom of endometrial cancer: a case report. Anticancer Res 2002;22(4):2531-2
4
5-Paulussen F, Leyendecker G, Windemuth W. Inguinal lymph node metastasis as primary symptom of endometrial carcinoma (author's transl). Geburtshilfe Frauenheilkd 1978;38(2):95-7.
5
Chambers JT, Carcangiu ML, Voynick IM, Schwartz PE. Immunohistochemical evaluation of estrogen and progesterone receptor content in 183 patients with endometrial carcinoma. Part II: Correlation between biochemical and immunohistochemical methods and survival. Am J Clin Pathol 1990;94(3):255-60.
6
7-Dabbs DJ, Sturtz K, Zaino RJ. The immunohistochemical discrimination of endometrioid adenocarcinomas. Hum Pathol 1996;27:172-7.
7
8-Berezowski K, Stastny JF, Kornstein MJ. Cytokeratins 7 and 20 and carcinoembryonic antigen in ovarian and colonic carcinoma. Mod Pathol 1996;9:426-9.
8
Takeshima N, Hirai Y, Tanaka N, Yamawaki T, Yamauchi K, Hasumi K. Pelvic lymph node metastasis in endometrial cancer with no myometrial invasion. Obstet Gynecol 1996;88(2):280-2.
9
ORIGINAL_ARTICLE
Kimura’s Disease: A Case Report and Review of Literatures
Kimura’s disease (KD) is a chronic inflammatory disorder primarily seen in male Asians during the second and third decades of life. Clinically, it presents as solitary or multiple subcutaneous nodules, predominantly in the head and neck region, typically in the pre auricular region, forehead, and scalp. The etiology of Kimura disease is still unknown. This disorder should be suspected when the clinical triad of painless unilateral cervical adenopathy, hypereosinophilia, and hyper-IgE is present. We report a case of KD with multiple subcutaneous nodules in the parotid, submandibular and posterior auricular regions, hypereosinophilia and hyper IgE levels.
https://ijp.iranpath.org/article_8344_99d97730cff4c8693cab87576592a118.pdf
2012-09-01
251
255
Kimura disease
Case Report
Jahanbanoo
Shahryari
jshahryari@gmail.com
1
Dept. of Pathology, Kerman University of Medical Sciences, Kerman, Iran
LEAD_AUTHOR
Shahriar
Dabiri
dabiri12@yahoo.com
2
Dept. of Pathology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Amin
Talebi
3
Dept. of Pathology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Kimura T, Yoshimura S, Ishikaura E. Unusual granulation combined with hyperplastic change of lymphatic tissue. Trans Soc Pathol Jpn 1948;37:179-80.
1
Sorbello M, Laudini A, Morello G, Sidoti MT, Maugeri JG, Giaquinta A, et al. Anaesthesiological implications of Kimura’s disease: a case report. J Med Case Reports 2009;25(3):7316.
2
Tseng CF, Lin HC, Huang SC, Su CY. Kimura’s disease presenting as bilateral parotid masses. Eur Arch Otorhinolaryngol 2005;262(1):8–10.
3
Motoi M, Horie Y, Akagi T. Kimura’s disease: clinical, histological, and immunohistochemical studies. Acta Med Okayama 1992;46:449-55.
4
Irish JC, Kain K, Keystone JS, Gullane PJ. Kimura’s disease: an unusual cause of head and neck masses. J Otolaryngol 1994;23:88-91.
5
Khoo BP, Chan R. Kimura disease: 2 case reports and a literature review. Cutis 2002 70(1):57-61.
6
Arshad AR. Kimura’s disease of parotid gland presenting as solitary parotid swelling. Head Neck2003;25(9):754-7.
7
Chen H, Thompson LD, Aguilera NS, Abbondanzo SL. Kimura disease: A clinicopathologic study of 21 cases. Am J Surg Pathol 2004;28(4):505-13.
8
Lee MW, Bae JY, Choi JH, Moon KC, Koh JK. Cutaneous eosinophilic vasculitis in a patient with Kimura's disease. J Dermatol 2004;31(2):139-41.
9
Buggage RR, Spraul CW, Wojno TH, Grossniklaus HE. Kimura disease of the orbit and ocular adnexa. Surv Ophthalmol 1999;44(1):79-91.
10
Ferri M, Ross C, Harvey JT. Recurrent orbital angiolym- phoid hyperplasia with eosinophilia (Kimura's disease). Can J Ophthalmol 1999;34(5):290-3.
11
Seregard S. Angiolymphoid hyperplasia with eosinophilia should not be confused with Kimura’s disease. Acta Ophthalmol Scand 2001;79(1):91-3. 13. Ray V, Boisseau-Garsaud AM, Hillion G. Kimura's disease on the hard palate in a patient from Martinique. Rev Med Interne 2003;24(4):253-6.
12
Meningaud JP, Pitak-Arnnop P, Fouret P, Bertrand JC. Kimura's disease of the parotid region: report of 2 cases and review of the literature. J Oral Maxillofac Surg 2007;65(1):134-40.
13
Yuen HW, Goh YH, Low WK, Lim-Tan SK: Kimura’s disease: a diagnostic and therapeutic challenge. Singapore Med J 2005;46(4):179-83.
14
Chusid MJ, Rock AL, Sty JR, Oechler HW, Beste DJ. Kimura’s disease: an unusual cause of cervical tumour. Arch Dis Child 1997;77(2):153–4.
15
Yeung E, Lih Ma. Bilateral Orbital Kimura's Disease in a Young Asian Man. Chang Gung Med J 2002:25(1);45-50.
16
ORIGINAL_ARTICLE
Asymptomatic Bronchogenic Cyst in a 35-Year-Old Woman as an Incidental Finding
Bronchogenic cyst is a result of abnormal development of the ventral foregut presents as a discrete mass. Most commonly, it is appeared in the middle mediastinum and rarely is seen in adults. We report a 35-year-old woman who was asymptomatic and incidentally was found with a mass in chest radiograph.
https://ijp.iranpath.org/article_8345_1adfbb4f3377300aa6eb8f7d2e859038.pdf
2012-09-01
256
258
Bronchogenic cysts
Case Reports
Iran
Sepideh
Siadati
siadati _sepideh@yahoo.com
1
Dept. of Pathology, Shahid Beheshti Hospital, Babol University of Medical Sciences, Babol, Iran
LEAD_AUTHOR
Seyed Reza
Modares
2
Dept. of Surgery, Shahid Beheshti Hospital, Babol University of Medical Sciences, Babol, Iran
AUTHOR
Akpinar L, Tufekcioglu O, Sen T, Basar N, Cagli KE, Aydog G, et al. Unusual case of bronchogenic cyst of interatrial septum related to transient ischemic attack. J Cardiovasc Med (Hagerstown). 2011;12(12):889-90.
1
Nino-Hernandez LM, Arteta-AcostaC, Redondo-de Oro K, Alcala-Cerral L, Redondo-Bermudez C, Marrugo- Grace O. Cervical bronchogenic cyst mimicking thyroglossal cyst: case report and literature review. Cir Cir 2011;79(4):330-3.
2
Cataletto ME. Bronchogenic cyst: eMedicine specialists>pediatrics: General Medicine>pulmonary. http://www.emedicine.com/PED/topic 2623.htm (6 october 2006).
3
Kumar V, Abbas AK, Fausto N, Aster JC. Robbins and cotran pathologic Basis of Disease. 8th ed. Philadelphia:Saunders Elsevier;2010.
4
Wick MR, powers CN. Silverbergs principles and practice of surgical pathology. 4th ed. Philadelphia:Churchill livingstone;2006.
5
St-Georges R, Deslauriers J, Duranceau A, Vaillancourt R, Deschamps C, Beauchamp G, et al. Clinical spectrum of bronchogenic cysts of mediastinum and lung in adult. Ann Thorac Surg 1991;52(1):6-13.
6
Kirmani B, Kirmani B, Sogliani F. Should asymptomatic bronchogenic cysts in adults be treated conservatively or with surgery? Interact Cardiovasc Thorac Surg 2010;11(5):649-59.
7
Laberge JM, Puligandla P, Flageole H. Asymptomatic congenital lung malformations. Semin Pediatr Surg 2005;14(1):16-33.
8
De Simone M, Cioffi U. Leiomyomas and extramucosal cysts of the esophagus in adults. The clinical picture and surgical therapy. Minerva Chir 1999;54(1-2):15-25.
9
AK togu S, Yuncu G, Halilcolar H, Ermete S, Buduneli T. Bronchogenic cysts: clinico pathological presentation and treatment. Eur Respir J 1986;(10): 2017-21.
10
Azeem F, Rathwell C, Awad WI. A near fatal presentation of a bronchogenic cyst compressing the left main coronary artery. J Thorac Cardiovase Surg 2008;135:1395-6.
11
Ubukata H, Satani T, Motohashi G, Konishi S, Goto Y, Watanabe Y, et al. Intra – abdominal bronchogenic cyst with gastric attachment: report of a case. Surg Today 2011;41(8):1095-100.
12
Shimazu R, Kuratomi Y, Inokuchi A. A case of an upper cervical bronchogenic cyst in an adult. Auris Nasus Larynx 2006;33(3):351-3.
13
Rasihashemi SZ, Sokouti M, Bozorgi F. A pitfall in the diagnosis of gaint bronchogenic cyst presented as loculated pleural effusion. Heart Lung Circ 2012;21(4):240-1.
14
Baumann CR, Konu D, Glatzel M, Siegel AM. Thoracolumbar intradural extramedullary bronchogenic cyst. Acta Neurochir (Wien) 2005;147(3):317-9.
15
Nistul M, Hardisson D, Riestra ML. Multiple pulmonary leiomyomatous hamartomas associated with a bronchogenic cyst in a man. Arch Pathol Lab Med 2003;127(4):e194-6.
16
ORIGINAL_ARTICLE
Primary Subcutaneous Hydatid Cyst in Scapula
Hydatid disease, caused by larval stage of Echinococcus granulosus, is a common parasitic infection of human and animal. Although liver and lung are the most commonly affected area, hydatid cyst may develop any part of the body. Primary subcutaneous hydatid cyst is extremely rare. We report a case of 54 years old man who presented with palpable mass in scapular region from 3 years ago. Present and past medical history was not significant finding. Pathologic evaluation identified the cystic structure as unilocular hydatid cyst. Primary hydatid disease constitutes a potentially serious differential diagnosis of any subcutaneous mass in endemic area of hydatid disease.
https://ijp.iranpath.org/article_8346_b37ad80e5ba3818cabd3072fa3065a67.pdf
2012-09-01
259
261
Hydatid Cyst
Subcutaneous Tissues
Iran
Tahereh
Mirzaei
labmirzaee@yahoo.com
1
Dept. of Parasitology, Kashan University of Medical Sciences, Kashan, Iran
LEAD_AUTHOR
Hossein
Hooshyar
2
Dept. of Parasitology, Kashan University of Medical Sciences, Kashan, Iran
AUTHOR
Henry JB. Clinical Diagnosis and Management by laboratory Methods. 21st ed. New York:Sunders;2007.
1
Mandell GI, Bennet JE, Dolin R, editors. Principles and practice of infectious disease. Philadelphia:Churchill Livingston Inc;2005.
2
Babu KS, Goel D, Prayaga A, Rao IS, Kumar A. Intra abdominal hydatid cyst: a case report. Acta Cytol 2008;52(4):464-6.
3
Dănilă N, Chifan M, Prescorniţă L, Andronic D, Taraşi C, Crumpei F. Rare location of a hydatid cyst- in the upper mediastinum migrating into the spinal channel. Rev Med Chir Soc Med Nat Iasi 2001;105(3):573-5.
4
Brunetti E, Kern P, Vuitton DA. Writing Panel for the WHO-IWGE. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 2010;114(1):1-16.
5
Akhbari Sh, Ehsanbakhsh AR, Khorashadizadeh N. Hydatid disease of the breast, case report. Ofogh-e-Danesh 2009;15(2):65-70.
6
Kireşi DA, Karabacakoğlu A, Odev K, Karaköse S. Uncommon locations of hydatid cysts. Acta Radiol 2003;44(6):622-36.
7
Memis A, Arkun R, Bilgen I, Ustun EE. Primary soft tissue hydatid disease: report of two cases with MRI characteristics. Eur Radiol 1999; 9(6):1101-3.
8
Orhan Z, Kara H, Tuzuner T, Sencan I, Alper M. Primary subcutaneous cyst hydatic disease in proximal thigh: an unusual localization: a case report. BMC Musculoskelet Disord 2003;7(4):25.
9
Sallami S, Ayari K, Oueslati B, Miladi M. Isolation of subcutaneous hydatid cyst. Tunis Med 2011;89(3):314-5
10
Al-Ibrahim J. Prevalence of hydatid cyst disease in west Bank-Palestine. An-Najah:National University publication;2009.
11
Sayek I, Onat D. Diagnosis and treatment of uncomplicated hydatid cyst of the liver. World J Surg 2001;25(1):21-7.
12
John TD, Petri WA. Markell and voges Medical Parasitology. 9th Ed. Missouri:Saunders;2006.
13
Chevalier X, Rhamouni A, Bretagne S, Martigny J, Larget-Piet B. Hydatid cyst of the subcutaneous tissue without other involvement: MR imaging features. AJR Am J Roentgenol 1994;163(3):645-6.
14
Kayaalp C, Dirican A, Aydin C. Primary subcutaneous hydatid cyst: A review of 22 cases. Int J Surg 2011;9:117-21.
15
Ousadden A, Elbouhaddouti H, Ibnmajdoub KH, Mazaz K, Aittaleb K. A solitary primary subcutaneous hydatid cyst in the abdominal wall of a 70-year-old woman: a case report. J Med Case Reports 2011;5(1):270.
16
Neumayr A, Troia G,de Bernardis C, Tamarozzi F, Goblirsch S, Piccoli L, et al. Justified concern or exaggerated fear: the risk of anaphylaxis in percutaneous treatment of cystic echinococcosis-a systematic literature review. PLoS Negl Trop Dis 2011;5(6):e1154.
17
Farzam R, Ghaempanah A, Feyzi A. Intramuscular hydatid cyst of the thigh: a case presentation. J Zanjan Univ Med Sci 2011;18(73):83-8.
18
Suryawanshi P, Khan AQ, Jatal S. Primary hydatid cyst of pancreas with acute pancreatitis. Int J Surg Case Rep 2011 2(6):122-4.
19
ORIGINAL_ARTICLE
Molecular Genetics Diagnosis of Wilson Disease: the First Reported Case of ATP7BGene Mutation at Codon 778 in Southwest Iran
Wilson disease is a metabolic disorder with an autosomal recessive genetic pattern and occurs in 1-4 of every 100000 individuals. Inactivation of the ATP7B gene leads to accumulation of the toxic copper to liver and brain causing hepatic and neurological complication. Therefore, most patients suffer from chronic hepatic inflammation and central nervous system disorder. Nowadays, up to 500 mutations were found in the ATP7B gene that weaken or fully disrupt the function of the gene product. Recurrent mutations were found in different population. We found a homozygous pathogenic missense mutation at codon 778 (R778W) in an individual from southwest Iran. This mutation has been reported in previous studies in the continents America and Europe. The present study is the first report from Wilson disease that has been diagnosed in southwest Iran. This mutation has been shown in previous studies in patients from continents America and Europe.
https://ijp.iranpath.org/article_8347_270f13b6b764e2b2a125217cd619e05e.pdf
2012-09-01
262
266
Wilson Disease
ATP7B Cu-binding P type ATPase
Iran
Hamid
Galehdari
galehdari187@yahoo.com
1
Dept. of Genetics, Shahid Chamran University, Ahvaz, Iran
LEAD_AUTHOR
Raheleh
Tangestani
2
Toxicology Research Centre, Jundishapur University of Medical Science, Ahvaz, Iran
AUTHOR
Brewer GJ. The risks of copper toxicity contributing to cognitive decline in the aging population and to Alzheimer’s disease. J Am C Nut 2009;28(3):238–42.
1
Gaw A, Murphy MJ, Cowan RA. “Copper,” in Clinical Biochemistry: An Illustrated Color Text. Philadelphia:Churchill Livingstone;2008.
2
Ala A, Walker AP, Ashkan K, Dooley JS, Schilsky ML. Wilson's disease. Lancet 2007;369(9559): 397–408.
3
Wilson SAK. Progressive lenticular degeneration: a familial nervous disease associated with cirrhosis of the liver. Brain 1912;34:295–508.
4
Bull PC, Thomas GR, Rommens JM, Forbes JR, Cox DW. The Wilson disease gene is a putative copper transporting P-type ATPase similar to the Menkes gene. Nat Genet 1993;5:327–337.
5
Nagano K, Nakamura K, Urakami K, Umeyama K, Uchiyama H, Koiwai K, et al. Intracellular distribution of the Wilson’s disease gene product (ATPase7B) after in vitro and in vivo exogenous expression in hepatocytes from the LEC rat, an animal model of Wilson’s disease. Hepatology 1998; 27(3):799–807.
6
Hung IH, Suzuki M, Yamaguchi Y, Yuan DS, Klausner RD, Gitlin JD. Biochemical characterization of the Wilson disease protein and functional expression in the yeast Saccharomyces cerevisiae. J Biol Chem 1997;272(34):21461–6.
7
Merle U, Schaefer M, Ferenci P, Stremmel W. Clinical presentation, diagnosis and long-term outcome of Wilson's disease: a cohort study. Gut. 2007;56(1):115–20.
8
Shiono Y, Wakusawa S, Hayashi H, Takikawa T, Yano M, Okada T, et al. Iron accumulation in the liver of male patients with Wilson disease. Am J Gastroenterol 2001;96(11):3147-51.
9
Kalach N, Seidman EG, Morin C, Rasquin-Weber A, O’Regan S, Laberge JM, et al. Acute liver failure from Wilson’s disease in a five year-old child. Can J Gastroenterol 1993;7:610-2.
10
Wilson DC, Phillips MJ, Cox DW, Roberts EA. Severe hepatic Wilson’s disease in preschool-aged children. J Pediatr 2000;137(5):719-22.
11
Milkiewicz P, Saksena S, Hubscher SG, Elias E. Wilson’s disease with superimposed autoimmune features: report of two cases and review. J Gastroenterol Hepatol 2000;15(5):570-4.
12
Yamaguchi Y, Heiny ME, Gitlin JD. Isolation and characterization of a human liver cDNA as a candidate gene for Wilson disease. Biochem Biophys Res Commun 1993;197(1):271-7.
13
Huster D. High copper selectively alters lipid metabolism and cell cycle machinery in the mouse model of Wilson disease. J Biol Chem 2007;282(11):8343-55.
14
Scheinberg IH, Sternlieb I, Schilsky M, Stockert RJ. Penicillamine may detoxify copper in Wilson’s disease. Lancet. 1987;2(8550):95.
15
Kodama H, Murata Y, Iitsuka T, Abe T. Metabolism of administered triethylene tetramine dihydrochloride in humans. Life Sci 1997;61(9):899-907.
16
Walshe JM, Dixon AK. Dangers of non-compliance in Wilson’s disease. Lancet. 1986;1(8485):845-7.
17
Zali N, Mohebbi SR, Esteghamat S, Chiani M, Haghighi MM, Hosseini-Asl SM, et al. Prevalence of ATP7B gene mutations in Iranian patients with Wilson disease. Hepatitis monthly. 2011;11(11):890-4.
18
ORIGINAL_ARTICLE
Beta-Thalassemia Carrier Detection by NESTROFT
https://ijp.iranpath.org/article_8348_6548822a50ebd2c73b3d0aed4dc80c2d.pdf
2012-09-01
267
267
Somsri
Wiwanitkit
somsriwiwan@hotmail.com
1
Wiwanitkit House, Bangkhae, Bangkok, Thailand
LEAD_AUTHOR
Viroj
Wiwanitkit
2
Wiwanitkit House, Bangkhae, Bangkok, Thailand
AUTHOR
Chakrabarti I, Sinha SK, Ghosh N, Goswami BK. Beta-Thalassemia Carrier Detection by NESTROFT: An Answer in Rural Scenario? Iranian J Pathol 2012; 7(1):19–26.
1
Wiwanitkit V. Using of osmotic fragility (OF) and dichlorophenol-indolephenol (DCIP) tests screening for antenatal clinic: appraisal of usefulness of the program in rural Thai communities. Rural Remote Health 2006;6(2):587.
2
Wiwanitkit V, Suwansaksri J, Paritpokee N. Combined one-tube osmotic fragility (OF) test and dichlorophenol-indolphenol (DCIP) test screening for hemoglobin disorders, an experience in 213 Thai pregnant women. Clin Lab 2002;48(9-10):525-8.
3
Wiwanitkit V. Combined osmotic fragility and dichlorophenol-indolphenol test for hemoglobin disorder screening in Thai pregnant subjects: an appraisal. Lab Hematol 2004;10(2):119-20.
4
Chapple L, Harris A, Phelan L, Bain BJ. Reassessment of a simple chemical method using DCIP for screening for haemoglobin E. J Clin Pathol 2006;59(1):74-6.
5