Serology Testing for SARS-CoV-2: Benefits and Challenges
Mohammadreza
Jalali Nadoushan
Department of Pathology, Shahed University, Tehran, Iran
author
Soha
Ahmadi
Department of Chemistry, University of Toronto, Toronto, Canada
author
Paniz
Jalali Nadoushan
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Canada
author
text
article
2020
eng
As COVID-19 was declared as a pandemic by the World Health Organization (WHO) in March 2020, it is an emerging need to discuss different aspects of this pandemic. In any pandemic, valid and rapid laboratory diagnostic tests are critically important for early diagnosis, which will increase the rate of successful treatment and more importantly prevent the spread of the disease.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
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no.
2020
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https://ijp.iranpath.org/article_39841_1b21d1906135fe80118b3cdbe19949d8.pdf
dx.doi.org/10.30699/ijp.2020.39841
The Novel Coronavirus SARS-CoV-2 Vulnerability Association with ABO/Rh Blood Types
Alireza
Abdollahi
Department of Pathology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
author
Maedeh
Mahmoudi-aliabadi
Department of Laboratory, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
author
Vahid
Mehrtash
Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Bita
Jafarzadeh
Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Mohammadreza
Salehi
Department of Infectious Diseases and Tropical Medicine, School of Medicine, Imam Hospital complex, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Background & Objective: Coronavirus disease 2019 (COVID-19) is the most recent emerging viral disease. Defining the epidemiological aspects and factors influencing the susceptibility of the patients to COVID-19 has been an ongoing struggle. In the present study, we have investigated the connection between ABO histo-blood group phenotypes and the COVID-19. Methods: This study was conducted on 397 patients with confirmed diagnoses of COVID-19 admitted to our center. Also, 500 individuals were selected to form the controls, all of whom had been disclosed to the same medical center in June 2019, before the onset of the outbreak. Results: Our results demonstrated ABO histo-blood phenotypes are correlated with patients’ susceptibility to the infection. A higher rate of infection was observed among patients with the AB histo-blood group, while patients with the O histo-blood group have shown a lower rate of infection. The Rh blood group phenotype was not statistically significant in determining a patient’s vulnerability. Conclusion: Similar to several previous studies about other viral diseases’ association with ABO histo-blood groups, we have concluded that an individual’s ABO histo-blood group phenotype and his/her susceptibility to COVID-19 are indeed connected. So far, only one research has been conducted about this association. Interestingly, while we observed a decreased vulnerability to the disease among patients with an O histo-blood group, we have reached discordant results regarding the increased susceptibility among individuals with an AB histo-blood group, unlike A histo-blood group in the previous study.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
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2020
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https://ijp.iranpath.org/article_39842_eae7669eee8d65cc47ff0c700c489e63.pdf
dx.doi.org/10.30699/ijp.2020.125135.2367
Necessity of Routine Repeat Testing of Critical Values in Various Working Shifts
Hiva
Saffar
Department of Pathology and Laboratory Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Alireza
Abdollahi
Department of Pathology and Laboratory Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Atefe Sadat
Hosseini
Department of Pathology and Laboratory Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Mojgan
Torabi Farsani
Department of Pathology and Laboratory Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Ghazal
Hajinasrollah
Department of Community Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Pegah
Mohaghegh
Department of Community and Preventive Medicine, Arak University of Medical Sciences, Arak, Iran
author
text
article
2020
eng
Background & Objective: Accurate and timely reporting of critical values is an important issue. There is some doubt whether repeat testing of critical values would offer any advantage over single testing or not. The aim of this study was evaluation of utility of routine repeat testing of critical values in our referral center and to compare probable variations in different working shifts. Methods: Clinical results of serum Potassium, Calcium, Blood Hemoglobin and Prothrombin Time (INR: International Normalized Ratio) were evaluated for three months. Results: Totally, 178, 96, 67 and 107 consecutive critical values for Potassium, Calcium, Hemoglobin and INR were reported, respectively. In potassium and Hemoglobin 5.05% and 1.17% of retest runs exceeded the acceptable tolerance limit. All of the calcium retest results were within the acceptable limit. For INR, 21/107 retest results did not meet the acceptable tolerance limit, nine still were critical. Afternoon working run performance was significantly better than the two others. Conclusion: Our observation suggests that routine repeat of hematology and chemistry critical test result is not necessary and may adversely affect patient safety measure. However, attention should be paid to results greater than analytical measurement range and all such results should be repeated before reporting.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
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2020
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https://ijp.iranpath.org/article_39843_f0be222ac2b949ca546284aa9fc9aa0c.pdf
dx.doi.org/10.30699/ijp.2020.99403.1980
Interobserver Agreement in Assessing Dysplasia in Colorectal Adenomatous Polyps: A Multicentric Iranian Study
Tahmineh
Mollasharifi
Department of Pathology, Clinical Research Development Center, Shahid Modarres Educational Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Mahsa
Ahadi
Department of Pathology, Shohada-e-Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Elena
Jamali
Department of Pathology, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Afshin
Moradi
Department of Pathology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Parisa
Asghari
Department of Pathology, Clinical Research Development Center, Shahid Modarres Educational Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Saman
Maroufizadeh
Department of Biostatistics, School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran
author
Behrang
Kazeminezhad
Department of Pathology, Clinical Research Development Center, Shahid Modarres Educational Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Background & Objective: Most colorectal cancers (CRCs) arise from adenomatous polyps, and clinical management of this type of polyp is highly dependent on the reliability and validity of the pathological diagnosis. The aim of this study was to examine the interobserver agreement of five pathologists in assessing dysplasia in adenomatous polyps. Methods: In this study, a total of 146 adenomatous polyps of patients undergoing colonoscopy were selected from hospitals of Shahid Beheshti University of Medical Sciences, Tehran, Iran between 2017 and 2018. Five pathologists independently classified adenomatous polyps according to histologic type, nuclear pseudostratification, mitotic activity, nuclear polarity, nuclear pleomorphism, nuclear shape, nucleolus, chromatin pattern, cytology grade, architectural features, dysplasia, and final diagnosis. The overall kappa statistic (k) was used to assess agreement among pathologists. Results: The mean age of the patients was 62.06 ± 13.06 (mean ± SD) with a male-to-female ratio of 2.2:1. The most common site of resection was the sigmoid colon (28.1%). The highest agreement was found for dysplasia grade (k=0.415) and histologic type (k=0.401), whereas the lowest agreement was found for mitotic activity (k=0.185), nuclear shape (k=0.187), and nucleolus (k=0.196). Conclusion: Our findings indicate that agreement among pathologists in assessing dysplasia in adenomatous polyps is within fair to moderate levels of agreement. Therefore, there is a vital need to better clarify the current diagnostic criteria.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
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no.
2020
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https://ijp.iranpath.org/article_39853_95f2e48a02a5b4305050ccb4c7a83932.pdf
dx.doi.org/10.30699/ijp.2020.115021.2250
The Survival of Patients with t(15;17)(q22;q12) Positive Acute Promyelocytic Leukemia: A Study in North-East of Iran
Hossein
Ayatollahi
Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ali
Bazi
Clinical Research Development Unit, Amir-Al-Momenin Hospital, Zabol University of Medical Sciences, Zabol, Iran
author
Mohammad Hadi
Sadeghian
Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ali
Fani
Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Payam
Siyadat
Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
author
Maryam
Sheikhi
Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Omolbanin
Sargazi-aval
Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Background & Objective: Acute promyelocytic leukemia (APL) with t(15;17)(q22;q12) is a relatively common subtype of acute myeloid leukemia (AML). Here, our objective was to ascertain the survival of patients with this leukemia in north-east of Iran. Methods: Survival rates of 42 APL patients with t(15;17)(q22;q12) were assessed. Clinical information was obtained from archived medical records. Statistical analysis was performed by SPSS 18 software using log-ranked test and Kaplan Maier survival analysis. Results: Females and males comprised 49% and 51%, respectively. The mean age at diagnosis was 34.3 ± 14.1 years old. During the study period, 17 demises occurred in males, while this number was 7 in females. The mean survival of patients (month) was 23.22 ± 3.57 (95% CI: 16.21 ± 30.2). The five-year survival rate obtained 30%. Regarding demographic and clinical features, the highest rates of 5-year survival were recorded in patients with 20-35 years old (47.6%), males (51%), white blood cell count <10±/l (48%), and platelet count >140 ± /l (100%). Conclusion: Younger age, lower WBC count and higher platelet count were significantly associated with longer survival in AML patients with t(15;17)(q22; q12).
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
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no.
2020
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https://ijp.iranpath.org/article_39854_46fc0465453397d920595d40e90c7b80.pdf
dx.doi.org/10.30699/ijp.2020.101417.2007
Cytogenetics and Revised International Staging System (R-ISS): Risk Stratification in Multiple myeloma - A Retrospective Study in Indian Population
Chethana Babu
Udupa
Department of Pathology, Kasturba Medical college Manipal, Manipal academy of higher education, Manipal, Karnataka, India
author
KARTHIK
UDUPA
Department of Medical Oncology, Kasturba Medical college Manipal, Manipal academy of higher education, Manipal, Karnataka, India
author
Ananth
Pai
Department of Medical Oncology, Kasturba Medical college Manipal, Manipal academy of higher education, Manipal, Karnataka, India
author
Prathika
Sherigar
Department of Medical Oncology, Kasturba Medical college Manipal, Manipal academy of higher education, Manipal, Karnataka, India
author
text
article
2020
eng
Background & Objective: Cytogenetic abnormalities in Multiple myeloma (MM) has emerged as the most important factor that determine the prognosis and survival. Fluorescence in situ hybridization (FISH) can detect a greater number of cytogenetic abnormalities as compared to conventional karyotyping and hence has become the standard test in determining genetic abnormalities in MM. The present study was planned as there is an unmet need to find out various cytogenetic abnormalities and to implement them in prognostic stratification by Revised International Staging System (R-ISS) among Indian population. Methods: A single institution retrospective study was conducted among a total of 117 patients newly diagnosed as Multiple Myeloma. They were analyzed for various cytogenetic abnormalities by using interphase FISH (iFISH) and were staged according to Revised International Staging System (R- ISS). Results: Out of the 117 patients studied, deletion 17p13 (p53) was present in 16 patients (13.67%). Thirty patients (25.64%) showed deletion 13q14.3. Three patients (2.56%) were detected to have t(4:14).Two patients (1.7%) had t(11:14) and t(14:16), respectively. Total of 19 patients (16.23%) in our study exhibited high risk cytogenetics and two among them had more than one high risk cytogenetic abnormalities. There was a 66.4% moderate correlation between ISS-III and high-risk cytogenetics which was statistically insignificant. Of the total 117 patients, 37 (31.62%) were staged R-ISS III. Conclusion: High risk cytogenetics was found in 16.23 % of our study population and del 17p13 was the most common high-risk cytogenetic abnormality. Of the studied subjects, 31.62% had R-ISS III, which is significantly higher compared to western population.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
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2020
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https://ijp.iranpath.org/article_39857_30c8142e245852d8246ed26d72c593dc.pdf
dx.doi.org/10.30699/ijp.2020.105128.2078
Detection of Apoptosis in Leukoplakia and Oral Squamous Cell Carcinoma using Methyl Green Pyronin and Hematoxylin and Eosin
Pooja
Sharma
Department of Oral Pathology, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India
author
Anjali
Narwal
Department of Oral Pathology, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India
author
Mala
Kamboj
Department of Oral Pathology, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India
author
text
article
2020
eng
Background & Objective: Cell population and turnover are controlled by a balance between cell proliferation and apoptosis. Detection of apoptosis in oral cancer contributes to its better prognosis and improved management. This study aimed to quantify apoptotic cells in leukoplakia and oral squamous cell carcinoma (OSCC) using methyl green-pyronin (MGP) and hematoxylin and eosin (H & E) staining. Methods: The sample included a total of 130 subjects (comprising 108 males and 22 females). Formalin fixed and paraffin embedded tissues were used and categorized into three groups of normal oral mucosa (n=10), leukoplakia with dysplasia (n=60), and OSCC (n=60). The number of apoptotic cells and apoptotic index (AI) were calculated after staining with MGP and routine H & E stained slides. Results: MGP stained the condensed chromatin of apoptotic cells. Statistically significant difference (P≤0.001) was observed among various study groups in terms of numbers of AI and apoptotic cells. Also, AI increased with increasing grades of dysplasia, and it was the highest in well differentiated OSCC. Results were statistically significant in both H & E and MGP stained sections (P≤0.001). A good correlation was found between MGP and H & E staining results. Conclusion: MGP is more specific and can lead to intense staining for chromatin in apoptotic cells. Accordingly, it can provide a good alternative to H&E in identifying apoptotic cells.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
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2020
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https://ijp.iranpath.org/article_39858_ef6c2e39a7f84497491804ba8fb9e39e.pdf
dx.doi.org/10.30699/ijp.2020.107263.2115
Histopathological Spectrum of Meningiomas with Emphasis on Prognostic Role of Ki67 Labelling Index
Girish
Solanke
Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104
author
Vidya
Monappa
Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal- 576104
author
Ranjini
Kudva
Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104
author
text
article
2020
eng
Background & Objective: Meningiomas are the most frequently encountered primary non-glial tumors of the central nervous system (CNS). The Ki67 labelling index (Ki67LI) is a proliferation marker that may prove useful in determining the histological grade. This study aims at: 1) Studying the frequency, grade and histomorphological spectrum of meningiomas, 2) Evaluating 20 histological parameters and determining its utility in grading meningiomas and 3) Comparing the Ki67LI in the various subtypes and WHO grades. Methods: The cases of meningiomas diagnosed in our Department from June 2009 to May 2014 were included. The clinical details, grade and 20 histological parameters: mitosis, vesicular nuclei, macronucleoli, nuclear pleomorphism, scattered bizarre nuclei, hypercellularity, sheeting, lymphocytes, small cell change, foam cells, ossification, necrosis, papillary change, lipidization, psammoma bodies, vascularization, brain invasion, dural invasion, bone invasion and other soft tissue invasion were recorded for each case. The average and highest Ki67LI was recorded as percentage and number per high power field. Results: A total of 175 cases of meningioma were included: grade I (145), grade II (30). Atypical histological features like hypercellularity, sheeting, etc. were common in grade II tumors. Increased vascularity, lymphocytes and psammoma bodies were common in grade I tumors. Ki67LI (highest) ranged from 1-6% in grade I and 5-12% in grade II tumors. Conclusion: Among different methods showing mitotic activity, Ki67% (highest) was the most statistically significant LI in differentiating grade I and grade II tumors. The median Ki67% (highest) was 4% for grade I and 7% for grade II tumors.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
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no.
2020
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https://ijp.iranpath.org/article_39859_3a620598f1cd2ded28e74f747f888e55.pdf
dx.doi.org/10.30699/ijp.2020.107195.2119
Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
Hadi
Hassibi
Department of Veterinary Surgery, Shahid Bahonar University of Kerman, Kerman, Iran
author
Alireza
Farsinejad
Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran
author
Shahriar
Dabiri
Pathology and stem cell Research Center, Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran
author
Dariush
Vosough
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
Abbas
Mortezaeizadeh
Pathology and stem cell Research Center, Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran
author
Reza
Kheirandish
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahnoar University of Kerman, Kerman, Iran
author
Omid
Azari
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2020
eng
Background & Objective:This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. Methods: In this experimental study, a critical size defect (CSD) (10 mm) was created in the radial diaphysis of 40 rabbits. In group A, the defect was left intact with no medical intervention. In group B, the defect was filled by a decellularized bone graft. In group C, the defect was implanted by a decellularized bone graft enriched with platelet growth factors. In group D, the defect was treated by a decellularized bone graft seeded by periosteal mesenchymal stem cells (MSCs). Also, in group E, the defect was filled by a decellularized bone graft enriched with platelet growth factors and periosteal MSCs. Radiological evaluation was done on the first day and then in the second, fourth, and eighth weeks after the operation. The specimens were harvested on the 28th and 56th postoperative days and evaluated for histopathological criteria. Results: The radiologic and microscopic analysis of the healing process in bone defects of the treated groups (C, D, and E) revealed more advanced repair criteria than those of groups A and B significantly (P<0.05). Conclusion: Based on this study, it appears that implantation of concentrated PSCs in combination with growth factors and allogeneic cortical bone graft is an effective therapy for the repair of large bone defects.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
15
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2020
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https://ijp.iranpath.org/article_39860_cf890cd9593b2b7d1317cd135c6f8a07.pdf
dx.doi.org/10.30699/ijp.2020.101715.2013
Predicting Effects of Clinicopathological Variables on Her2 Gene Amplification by Chromogenic in situ Hybridization (CISH) in IHC Her2 (2+) Breast Cancer Patients; A Study from Iran
Mohammad
Hashemi-Bahremani
Department of Pathology, Imam Hosein Educational Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Abdolali
Ebrahimi
Department of Pathology, Imam Hosein Educational Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Mohaddese
Fallahi
Department of Pathology, Imam Hosein Educational Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Background & Objective: The her2 amplification plays an important role in breast cancer management. Therefore, there is a need for using supplementary molecular methods in IHC equivocal cases. Present study has been conducted to determine the effects of clinicopathological variables on her2 gene amplification by chromogenic in situ hybridization (CISH) in IHC Her2 (2+) breast cancer individuals. Methods: A cross-sectional study was conducted in Zaferanyeh Laboratory collaborated with Shahid Beheshti University of Medical Sciences (Tehran-Iran; 2015-2018). All pathological data related invasive breast cancer patients with equivocal IHC results were included. CISH method was performed as a supplementary technique. The associations between histopathologic variables, status of Ki-67 index, progesterone and estrogen receptors (PR & ER) with her2 amplification by CISH were investigated and analyzed. The level of significance was considered as P-value < 0.05. Result: Totally, 239 patients with mean age of 53.2 years were studied. CISH identified her2 gene amplification in 51 subjects (21.3%). The type of tumor (invasive ductal carcinoma), the tumor grade, and the value of Ki-67 index were directly correlated with her2 amplification. Significant negative associations were also observed between CISH results and ER and PR expression. Conclusion: As her2 gene amplification was identified in 21.3% of invasive breast cancer patients with equivocal IHC results, it is supposed that applying CISH method may consider as a potentially valuable supplementary method. Results have also shown that higher grades of tumor, invasive ductal carcinoma, absences of hormone receptors and high Ki-67 index significantly correlated with the her2 amplification.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
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2020
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https://ijp.iranpath.org/article_39872_27b59c729e38b4099e10cc1a354d346c.pdf
dx.doi.org/10.30699/ijp.2020.110293.2172
Expression Pattern of Telomerase Reverse Transcriptase (hTERT) Variants and Bcl-2 in Peripheral Lymphocytes of Systemic Lupus Erythematosus Patients
Armin
Attar
Department of Cardiovascular Medicine, Division of Interventional Cardiology, Shiraz University of Medical Sciences, Shiraz, Iran
author
Mohsen
Khosravi Maharlooei
Students’ Research Committee, Cell and Molecular Medicine Research Group, Shiraz University of Medical Sciences, Shiraz, Iran
author
Mohammad
Nazarnia
Internal Medicine Department, Rheumatology Division, Shiraz University of Medical Sciences, Shiraz, Iran
author
Ahmad
Hosseini
Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran
author
Zohre
Bajalli
Students’ Research Committee, Cell and Molecular Medicine Research Group, Shiraz University of Medical Sciences, Shiraz, Iran
author
Yalda Sadat
Moeini
Department of Anesthesiology, Division of Intensive Care Unit, Shiraz University of Medical Sciences, Shiraz, Iran
author
Ahmad
Monabati
Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
author
Fatemeh
Amirmoezi
Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
author
Mansooreh
Jaberipour
Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran
author
Mojtaba
Habibagahi
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
author
text
article
2020
eng
Background & Objective: It is not clear whether activated lymphocytes of patients with systemic lupus erythematosus (SLE) are more proliferative or less apoptotic. We aimed to delineate potential differences between B and T cells of SLE patients compared to healthy controls regarding the telomerase activity and apoptosis status. Methods: In this cross-sectional case control study, Blood samples were taken from 10 SLE patients and 10 healthy controls. B and T cells were separated using magnetic cell sorting system. Telomeric repeat amplification protocol (TRAP) assay and real-time PCR were used to determine the telomerase activity and the expression of alternatively spliced variants. Result: Four patients under treatment showed significant telomerase activity in their T cells. Four of the newly diagnosed patients showed telomerase activity in their B cells (20% of all patients and 40% of new onset patients). There was no specific pattern of human telomerase reverse transcriptase variant expression within the patients’ lymphocytes. A significantly reduced expression of Bcl-2 was detected in B cells (P=0.018) and a trend toward lower Bcl-2 expression in T cells was seen in SLE patients compared to healthy controls. Conclusion: Although not definitive, our results may suggest that B cells may have more active roles during the earlier phases of the disease attack, while T cells take over when the disease reaches its chronic stages.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
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2020
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https://ijp.iranpath.org/article_39898_ff8984e9827db716827764dca460ba1f.pdf
dx.doi.org/10.30699/ijp.2020.110994.2187
Diagnostic Value of Cytokeratin 34 beta E12 (Ck34βE12) and α-Methylacyl-CoA racemase (AMACR) Immunohistochemical Expression in Prostatic Lesions
Ikram A.
Hasan
Pathology and Forensic Medicine Department, College of Medicine, Mustansiriyah University, Baghdad, Iraq
author
Hiba
Gaidan
Pathology and Forensic Medicine Department, College of Medicine, Mustansiriyah University, Baghdad, Iraq
author
Methaq
Al-kaabi
Pathology and Forensic Medicine Department, College of Medicine, Mustansiriyah University, Baghdad, Iraq
author
text
article
2020
eng
Background & Objective:Some prostatic lesions contain small suspicious foci for prostatic carcinoma in which the morphological features are equivocal. Two immunohistochemical markers namely, cytokeratin 34 beta E12 (Ck34βE12) and α-Methylacyl-CoA racemase (AMACR), were evaluated in these lesions for a definitive diagnosis and avoiding misdiagnosis or overdiagnosis of prostatic carcinoma. Methods:A total of 90 paraffin embedded blocks of prostatic tissue were selected and categorized into three groups as follows: 50 cases of benign prostatic hyperplasia (BPH), 20 cases of prostatic carcinoma, and 20 cases of benign prostatic lesions with suspicious foci labeled as ASAP (atypical small acinar proliferation) that occupy not more than 5% of the lesion. These cases were revised for histopathological diagnosis and stained with two immunohistochemical markers: Ck34βE12 and AMACR. Result:While 92.9% of BPH were positive for Ck34βE12, 96% of prostatic carcinoma were negative for this marker (P=0.0001). Regarding AMACR, 92.9% of BPH cases were negative, but 92% of prostatic carcinoma cases were positive for this marker (P=0.0001). Out of 20 cases of BPH, 15 cases containing suspicious foci showed Ck34βE12+/AMACR- (diagnosis: benign), but 5 cases were Ck34βE12-/AMACR+, for which the diagnosis changed to prostatic carcinoma (P=0.04). Conclusion :Immunohistochemical staining with Ck34βE12 and AMACR improved the diagnostic performance and also increased confidence level for establishing definite diagnosis in cases with suspicious foci, in which the morphological features were equivocal. This could help to avoid misdiagnosis or overdiagnosis of prostatic carcinoma that would eventually improve the management of the patient and subsequently the prognosis.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
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2020
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https://ijp.iranpath.org/article_39901_1028d3501f59f3df665b02f6ac29ebbf.pdf
dx.doi.org/10.30699/ijp.2020.113544.2229
Androgen Receptor Expression and Its Correlation with Clinicopathological Parameters in Iranian Patients with Triple Negative Breast Cancer
Azar
Naimi
Department. of Pathology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Maryam
Sultan
Department. of Pathology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Elham
Amjadi
Department. of Pathology, Poursina Hakim Digestive Diseases Research Center, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Parvin
Goli
Department. of Pathology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Amirhosein
Kefayat
Department of Oncology, Cancer Prevention Research Center, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
text
article
2020
eng
Background & Objective: Our knowledge about correlation of androgen receptor expression and clinicopathological properties of triple-negative breast cancer (TNBC) patients is inadequate, particularly in the Iranian population. The main aim of the present study was to assess the AR expression in TNBC Iranian patients and evaluate its correlation with their clinicopathological parameters. Methods: Herein, 76 TNBC patients were evaluated for the AR expression by immunohistochemistry. The slides' staining intensity was investigated according to the average degree of nuclear staining and sub-classified into negative (0), weak (1), moderate (2), or strong (3). Subsequently, the positive cells percentage for each slide was assessed and sub-classified into <25% (1), 25-50% (2), 50-75% (3), and >75% (4). The aggregation of these two scores was used as the final score ranging from 0 to 7. While 4-7 scores were selected as positive, the others were included in the AR-negative expression group. Fisher's exact test was used to analyze the AR expression correlation with the clinicopathological parameters. Result : Positive immunoreactivity for AR was observed in 8 out of 76 (11%) specimens. No-correlation (P>0.05) was observed between the AR expression and grade, stage, lymph node status, and Ki-67 level. The AR-positive patients exhibited older age at the time of diagnosis (P=0.0339) and larger tumor size (P=0.0224) in comparison with the AR-negative patients. Low percentage of TNBC patients expressed AR and no significant correlation was observed between its expression and most of the clinicopathological parameters. Conclusion : AR may not be a suitable biomarker and treatment target for the Iranian patients with TNBC.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
15
v.
3
no.
2020
239
244
https://ijp.iranpath.org/article_39902_8abbb5c425f6356f7d285aaf04c13669.pdf
dx.doi.org/10.30699/ijp.2020.112819.2224
Cyclin D1 Expression in Patients with Laryngeal Squamous Cell Carcinoma
Vahid
Zand
Department of Otolaryngology, Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
author
Fariba
Binesh
Dept. of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
author
Mojtaba
Meybodian
Dept. of Otolaryngology, Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
author
Farzan
Safi Dahaj
Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
author
Arezoo
Alamdar yazdi
Dept. of Otolaryngology, Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
author
text
article
2020
eng
Background & Objective: Laryngeal squamous cell carcinoma (LSCC) is considered to be one of the most common cancers of the head and neck, accounting for roughly 90% of all malignant tumors of the larynx. To have a timely diagnosis for a better and practical therapy, molecular markers have to be investigated. The aim of this study was to determine the expression of Cyclin D1 (CD1) in patients with laryngeal squamous cell carcinoma. Methods: In this study the demographic data of 82 patients with laryngeal squamous cell carcinoma, including age, gender and geographical region history of smoking and drug abuse, paraclinical findings, surgical description, and pathologic reports were extracted from their medical records. The stage and grade of the disease and tumor location were determined using their medical records. An appropriate tissue sample was selected. Then, the selected cancerous tissue samples stored as formalin-fixed paraffin-embedded tissue then were (Immunohistochemistry) IHC stained and analyzed in terms of the expression of CD1. Result & Conclusion : According to the results, 75 out of 82 (91.5%) investigated samples were positive for CD1 expression. There was a significant relationship between stage of the disease (P=0.041) and CD1 expression in patients with laryngeal squamous cell carcinoma. There was no significant relationship between gender (P=0.055), age (P=0.256), history of smoking and drug abuse (P=0.192), location of the tumor (P=0.90), grade of the disease (P=0.515) and geographical region (P=0.466) and CD1 expression in patients with laryngeal squamous cell carcinoma. The results of the present study showed that CD1 expression was higher (91.5%) in patients with laryngeal squamous cell carcinoma in comparison to the other studies. According to the results we can conclude that stage of the disease can significantly affect CD1 expression in patients with squamous cell carcinoma.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
15
v.
3
no.
2020
245
250
https://ijp.iranpath.org/article_39904_7b5a9dadc89872171b7c3128d7fb74de.pdf
dx.doi.org/10.30699/ijp.2020.116579.2276
Salivary Duct Carcinoma with Late Distant Brain and Cutaneous Metastasis: A Case Report
Hassan
Mir Mohammad Sadeghi
Oral & Maxillofacial Surgery Department, Taleghani Hospital, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Abbas
Karimi
Oral & Maxillofacial Surgery Department, Shariati Hospital, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
author
Amirsina
Rahpeima
School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
author
Samira
Derakhshan
Oral and Maxillofacial Pathology Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Salivary duct carcinoma (SDC) is a rare and highly aggressive salivary gland tumor with poor prognosis, rapid growth, distant metastasis, early regional metastasis, and a high rate of recurrence. The parotid gland is the most common site of involvement, and the lungs and the bones are the most common sites of distant metastasis of SDC. Herein, we present a case of SDC of the parotid gland in a 62-year-old male patient with an unusual metastasis to the skin of the primary site and brain 6 years after primary treatment, which comprised of total parotidectomy and radical neck dissection followed by radiotherapy. It is noteworthy that in few cases of SDC with infiltration, (and not in low-grade intraductal carcinoma of the salivary glands), routine treatment may not suffice, and long-term follow-up is highly recommended.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
15
v.
3
no.
2020
251
255
https://ijp.iranpath.org/article_39905_eced4306b6d91a60834ddd35f9dbc02c.pdf
dx.doi.org/10.30699/ijp.2020.103326.2039
Respiratory Epithelial Adenomatoid Hamartoma: A Very Rare Entity Originating from the External Auditory Canal
Behrouz
Shokouhi
Department of Pathology, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
author
Parisa
Mehrasa
Department of Pathology, Emam Reza Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
author
Farzad
Aslani
Department of Internal Medicine, Emam Ali Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
author
text
article
2020
eng
Respiratory epithelial adenomatoid hamartoma (REAH) is an uncommon lesion of the upper aerodigestive tract. It is characterized by abnormal glandular formations with ciliated epithelium arising from the nasal cavity, paranasal sinuses and nasopharynx, but other locations have also been described. We did not find any other cases of this hamartomas in the external auditory canal in the literature. A 40-year-old man was referred to the otorhinolaryngology department due to otorrhea and decreased hearing from 1 year ago. Otoscopic examination showed a reddish hemorrhagic mass in the left external auditory canal. Intraoperatively, the tumor was completely resected. The pathologic findings were consistent with REAH.
Iranian Journal of Pathology
Farname Inc in collaboration with Iranian Society of Pathology
1735-5303
15
v.
3
no.
2020
256
258
https://ijp.iranpath.org/article_39906_6e3473487ce33817be7db6fd5f0debf9.pdf
dx.doi.org/10.30699/ijp.2020.115463.2255