Bone & Soft tissue Pathology
Hadi Hassibi; Alireza Farsinejad; Shahriar Dabiri; Dariush Vosough; Abbas Mortezaeizadeh; Reza Kheirandish; Omid Azari
Abstract
Background & Objective:This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. Methods: In this experimental study, ...
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Background & Objective:This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. Methods: In this experimental study, a critical size defect (CSD) (10 mm) was created in the radial diaphysis of 40 rabbits. In group A, the defect was left intact with no medical intervention. In group B, the defect was filled by a decellularized bone graft. In group C, the defect was implanted by a decellularized bone graft enriched with platelet growth factors. In group D, the defect was treated by a decellularized bone graft seeded by periosteal mesenchymal stem cells (MSCs). Also, in group E, the defect was filled by a decellularized bone graft enriched with platelet growth factors and periosteal MSCs. Radiological evaluation was done on the first day and then in the second, fourth, and eighth weeks after the operation. The specimens were harvested on the 28th and 56th postoperative days and evaluated for histopathological criteria. Results: The radiologic and microscopic analysis of the healing process in bone defects of the treated groups (C, D, and E) revealed more advanced repair criteria than those of groups A and B significantly (P<0.05). Conclusion: Based on this study, it appears that implantation of concentrated PSCs in combination with growth factors and allogeneic cortical bone graft is an effective therapy for the repair of large bone defects.
Masood Saleem Mir; Mohammad Maqbool Darzi; Hilal Musadiq Khan; Shayaib Ahmad Kamil; Asif Hassan Sofi; Sarfaraz Ahmad Wani
Volume 9, Issue 2 , April 2014, , Pages 197-112
Abstract
Background & Objectives: Alloxan & streptozotocin are used for inducing diabetic models. Their combination has been used to reduce the individual chemical dosage and minimize the side effects. Present investigation was aimed at studying pre-diabetic clinical changes induced by low doses of Alloxan-STZ ...
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Background & Objectives: Alloxan & streptozotocin are used for inducing diabetic models. Their combination has been used to reduce the individual chemical dosage and minimize the side effects. Present investigation was aimed at studying pre-diabetic clinical changes induced by low doses of Alloxan-STZ cocktail in rabbits.
Methods: New Zealand White rabbits, 1-1.5 kg body weight, were administered alloxan (@50 mg/kg b.w.) and STZ (@ 35mg/kg b.w.) cocktail, as single intravenous dose. Blood glucose levels were monitored (0 h, 20 min, 1 h, and then hourly up to 9 h) and clinical signs noted. Rabbits surviving up to 9 hours were given glucose therapy.
Results: The cocktail caused immediate transient hypoglycaemia, followed by hyperglycaemia, and then progressively severe hypoglycaemia. Hypoglycaemia caused characteristic behavioural alterations from lethargy, through aesthesia, muscular weakness to recumbency. Severely affected rabbits revealed intermittent convulsions and died in coma.
Conclusion: Low dose Alloxan-STZ cocktail induced triphasic immediate response in rabbits. The behavioural changes reflected glycaemic status serving as a guide for institution of glucose therapy.
Shahriar Dabiri; Hamid Najafipour; Saeed Niazmand; Hamid Tabrizchi
Volume 1, Issue 2 , April 2006, , Pages 49-54
Abstract
Background and Objectives: The cause and pathophysiology of rheumatoid arthritis has not been fully understood and an experimental model of this disease is essential for research on the problem. In this research study, establishment and histopathological changes of chronic arthritis due to intra-articular ...
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Background and Objectives: The cause and pathophysiology of rheumatoid arthritis has not been fully understood and an experimental model of this disease is essential for research on the problem. In this research study, establishment and histopathological changes of chronic arthritis due to intra-articular antigen injection was used as a model of experimental rheumatoid arthritis. Materials and Methods: Thirty three New-Zeeland white rabbits were sensitized by subcutaneous injection of combination of methylated bovine serum albumin (MBSA) and Freund’s complete adjuvant (FCA) at days 1 and 14. Sensitized animals at day 28 received intra-articular injections of MBSA. At days 7, 14, 21, and 28 post-injection, excised knee joints were investigated for routine light microscopic changes. Results: It was found out that at day 7 there are fibrinous exudates in the joint space and pericapsular soft tissue, edematous synovial villi, and an intact cartilaginous site of joint. At day 14, lymphoid follicle formation at pericapsular area, short and widening of synovial villi, superficial erosion of joint cartilage (perichondritis) was observed. Thereafter, at day 21 increased secondary lymphoid follicles with active germinal centers at pericapsular areas, papillary hyperplasia of the synovial villi, thinning of the cartilaginous site of joint with mononuclear cellular infiltrates (chondritis) was noted. In addition, day 28 was demarcated by continuation of the chondritis and beginning of osteitis, granulation tissue formation (Pannus) at cartilaginous site of joint, and fibrotic changes of the synovial villi. Rare findings including pseudocyst space and palisading ranuloma at the pericapsular area was also observed. Conclusion: Antigen-induced chronic arthritis in the knee joint of the rabbit is a good experimental model to evaluate the pathogenesis and/or effects of drug interferences in the rheumatoid arthritis.