Uropathology
Omid Azari; Reza Kheirandish; Mohammad Farajli Abbasi; Shahin Ghahramani Gareh Chaman; Masoud Bidi
Abstract
Background: Renal ischemia reperfusion injury may occur in a variety of clinical situations, following a transient drop in total or regional blood flow to the kidney. This study was performed to investigate the protective effects of different antioxidants such as vitamin C, vitamin E, hydrocortisone ...
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Background: Renal ischemia reperfusion injury may occur in a variety of clinical situations, following a transient drop in total or regional blood flow to the kidney. This study was performed to investigate the protective effects of different antioxidants such as vitamin C, vitamin E, hydrocortisone and combination of these agents against experimental renal ischemia-reperfusion injury.
Method:Thirty male rats were divided into six groups. Group Sham, Group I/R: (45 min of ischemia followed by 1h of reperfusion), Group I/R+Vit C: (50 mg/kg Vit C, IV, immediately after reperfusion), Group I/R+Vit E: (20 mg/kg Vit E, IM, 15 min before reperfusion), Group I/R+Hydrocortisone: (50 mg/kg, IV, immediately after reperfusion), and Group Combination: Ischemia-reperfusion plus combination of Vit C, E and hydrocortisone. After the experiments, the left kidney was removed and the tissues were processed for histopathological examination.
Result: Severe injuries such as necrosis of tubules, atrophy of glomerulus, and hemorrhage were observed in group I/R. Histological scores indicating tissue injury significantly decreased in all treatment groups compared to the group I/R. The renal tissue in group treatment was preserved in comparison with the group I/R. Comparison between the treatment groups showed that group combination was more effective and group vit E was less effective in protecting of renal tissue against I/R injuries.
Conclusion: The results demonstrated simultaneous administration of combination of Vit C, E and hydrocortisone before reperfusion of blood flow to the ischemic tissue could show a synergy against deleterious effects of I/R injuries in kidney.
Sedigheh Siahkoohi; Mortez Anvari; Mahmood Akhavan Tafti; Mohammad Hosseini-sharifabad
Volume 9, Issue 2 , April 2014, , Pages 89-98
Abstract
Background and Objectives: Acrylamide is a monomer which is formed in foodstuffs containing carbohydrates altered to asparagine during thermal processing. Vitamin E is a component in human diet considered as the most effective lipid-soluble antioxidant found in the biological system. It prevents ...
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Background and Objectives: Acrylamide is a monomer which is formed in foodstuffs containing carbohydrates altered to asparagine during thermal processing. Vitamin E is a component in human diet considered as the most effective lipid-soluble antioxidant found in the biological system. It prevents initiation of oxidative tissue damage. The aim of this study was to investigate the potential role of vitamin E on hepatic biochemical and histological integrity in male mice fed with acrylamide. Materials and Methods: Twenty-eight adult male mice were randomly divided into four groups comprised of seven mice each. The first group served as control fed on ad-libitum diet; second group received 10 mg/kg/day acrylamide in drinking water; in third group, 100 mg/kg/day vitamin E was injected intraperitoneal, and fourth received a combination of acrylamide/vitamin E for 35 days. After cutting liver, liver injury was assessed by hematoxylin and eosin, and reticulin staining. Results: Following acrylamide consumption, the serum levels of liver enzymes significantly increased and light microscopy showed lymphocytes infiltration, inflammation of portal space and central vein, apoptosis, chromatolysis and fibrous expansion in some portal areas in acrylamide-treated mice. There was a statistically considerable difference between biochemical parameters, index apoptosis and histological features when the acrylamide plus vitamin E-treated group was compared with acrylamide-treated group. Conclusion: Acrylamide induced disturbance in hepatocytes activity and increased the serum levels of liver and structural changes in the liver. Administration of vitamin E significantly reduced the increased level of serum aminotransferase and the pathological changes, also effectively suppressed the acrylamide–induced liver injury.
