Microbiology
Sina Neshat; Padideh Daneii; Negar Neshat; Sina Raeisi; Saba Raeisi; Seyed Mohammad Malakooti; Noushin Afshar Moghadam
Abstract
Human papillomavirus infection is one of the most common genital infections. More than 100 types of the virus have been identified, most of which can infect the genital mucosa. The virus is associated with cancerous and precancerous lesions of the cervix. Some types, such as human papillomavirus 16 and ...
Read More
Human papillomavirus infection is one of the most common genital infections. More than 100 types of the virus have been identified, most of which can infect the genital mucosa. The virus is associated with cancerous and precancerous lesions of the cervix. Some types, such as human papillomavirus 16 and 18, are highly carcinogenic; some other types, such as human papillomavirus 6 and 11, are mildly carcinogenic, with HPV 31.33 in between. This study describes the relationship between different types of HPV infection and the findings of a Pap smear. In this prospective study, 1,500 samples from patients who admitted to a private Pathology Laboratory in Isfahan were collected during the years 2019-2020. Two samples were collected from each patient, one for Pap smear study and the other for polymerase chain reaction (PCR) test to detect different types of human papillomavirus (HPV) infection. In a study of 1,500 samples, 236 were positive for Papillomavirus (HPV) infection in Pap smear. By examining the genotype of positive examples, it was found that 14.8% of the samples were infected with type 16, and 1.7% were infected with type 82. HPV infection is common in Iran and is almost similar to European countries such as Germany and Spain. We also found that using a polymerase chain reaction (PCR) method to detect HPV viruses in vaginal secretions could be very useful. Our findings also show which disease is most associated with each type of HPV.
Cytology
Fatemeh Samiee Rad; mehdi Ghaebi; Simin Zarabadipour; Arezoo Bajelan; fatemeh Pashazade; Mehri Kalhor; Amane Barikani
Abstract
Background & Objective: Premalignant lesions of cervix have increased dramatically in recent years. Early diagnosis and management of abnormalities have an effective role in preventing the invasion of the disease and also in timely treatment. This study aimed to compare diagnostic methods in the ...
Read More
Background & Objective: Premalignant lesions of cervix have increased dramatically in recent years. Early diagnosis and management of abnormalities have an effective role in preventing the invasion of the disease and also in timely treatment. This study aimed to compare diagnostic methods in the detection of squamous cell abnormalities with abnormal Pap smear test. Methods: This cross-sectional study was performed on 1000 women with abnormal Pap smears in 2007-2018. Sampling was performed with simple method. All samples were subjected to an immediate assessment of colposcopy and histopathology if suspected. The checklist included demographic information as well as symptoms, cytopathology, colposcopy and histopathology findings. Data analysis was performed using descriptive and statistical analysis (P<0.05). Result: A significant relationship between histopathology and Pap smear findings was found (P=0.009), also there was a significant correlation between histopathology and colposcopy findings (P=0.001). However, there was no significant relationship between clinical symptoms and histopathology findings (p=0.8). Sensitivity , specificity , positive and negative predictive value of Pap smear were 43%, 65.9%, 75.4%, 32.2% and of colposcopy were 74.7%, 39.5%, 75%, 39.1%, and of clinical symptoms were 72.6%, 28.1%, 71.1%, 29.7%, respectively. Conclusion: Pap smear findings have the appropriate diagnostic accuracy in comparison with colposcopy and histopathology findings for screening and diagnosis of squamous intra-epithelial lesions. Also, there was higher sensitivity of colposcopy compared with Pap smear to detect cervical lesions. Therefore, it is advisable to use these methods simultaneously.
Afsaneh Ghasemi; Ali Davati; Saloumeh Peyvandi
Volume 1, Issue 3 , June 2006, , Pages 109-112
Abstract
Background and Objective: Cervical cancer involves many women annually and Pop smear test has played a significant role in reducing its mortality and for this reason, its improvement is very essential. In this respect, cervex brush is a new tool that has been introduced in many countries. Therefore, ...
Read More
Background and Objective: Cervical cancer involves many women annually and Pop smear test has played a significant role in reducing its mortality and for this reason, its improvement is very essential. In this respect, cervex brush is a new tool that has been introduced in many countries. Therefore, this study was conducted to compare Pop smears from cotton swab-spatula and Cervex brush methods with regard to cell number. Materials and Methods: The clinical trial and randomized protocol of this study was conducted on 400 women as referrals of gynecology clinic of Hazrat Rassoul Akram (s) hospital and the cases with inclusion criteria were further investigated and their data using above-mentioned methods were compared. For statistical analysis, SPSS software and student’s t-test and chi-square tests were used. Results: It was found out that the mean age of cases was 34.13 ± 9.3 years. Meanwhile, there was a significant difference between the groups regarding endocervical cells (p<0.001) and bleeding on sampling (p<0.001). Conclusion: It is concluded that appropriate use of Cervex brush method can prevent the need for re-sampling regarding Pop smear test and in this way it can lower health-related costs.
