Gynecologic Pathology
Mahdi Ahadi; Vahid Naseh; Masoud Salehipour
Abstract
Background & objective: The HER-2 gene is an important on co protein overexpressed in many types of cancers. The current study hypothesized that curcumin downregulates HER-2 and inhibits the signal transduction pathway of PI3K/Akt, MAPK, and activation of NFκB, which could be useful to treat ...
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Background & objective: The HER-2 gene is an important on co protein overexpressed in many types of cancers. The current study hypothesized that curcumin downregulates HER-2 and inhibits the signal transduction pathway of PI3K/Akt, MAPK, and activation of NFκB, which could be useful to treat overexpressed-HER-2 hepatocellular carcinoma (HCC). Methods: In the current study, 40 male NMRI (Naval Medical Research Institute) mice were divided into 4 groups of 10 as follow: Group1 (control group) only received 5 mL/kg corn oil, group 2 (poisoned group) received 30 mg/L arsenic (As2O3) dissolved in water, group3 (curcumin treated), and group 4 (curcumin and arsenic treated) received 10 to 20mg/5mL/kg for 60 days. Once experimental period was completed, liver samples were collected. The analysis of the gene expression was performed by real-time polymerase chain reaction (PCR) technique. Results: Gene expression analysis showed that curcumin had significantly downregulated the activity of HER-2, in poisoned mice. Conclusion: According to the current study results, it could be concluded that curcumin has the inhibitory potential toward HER-2-overexpressed HCC.
Hamid Galehdari; Fariborz Soheili; Ali Mohammad Foroughmand; Abdolreza Masjedizadeh
Volume 5, Issue 3 , June 2010, , Pages 116-120
Abstract
Objectives and Background: Mutation directed inactivation of the tumor suppressor gene p53 have been found incountries with high frequency for hepatocellular carcinomas (HCCs). Our goal in the present study was screening of the p53 gene in tumor tissues from HCC affected individuals in southwest ...
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Objectives and Background: Mutation directed inactivation of the tumor suppressor gene p53 have been found incountries with high frequency for hepatocellular carcinomas (HCCs). Our goal in the present study was screening of the p53 gene in tumor tissues from HCC affected individuals in southwest Iran for putative mutations in exons 7 and 8 that are known as hot spot regions. Materials & Methods: A total of 25 archival formalin fixed paraffin embedded samples prepared from 1997 to 2006 were collected from hospitals in southwest and northwest of Iran. We examined the codon 249 within the exon 7 using RFLP as well the full-length sequencing of exons 7 and 8. Results: The samples were diagnosed as HCC and classified in four groups, well differentiated (39%), moderately differentiated (54%), poorly differentiated (4.5%) and undifferentiated (2.5%). The hepatitis B virus (HBV) was detected in 16% (n=7) and 11% (n=5) of patient’s sera that were affected with liver cirrhosis. No patient was infected with hepatitis C virus. However, in one tumor sample a homozygote mutation was detected at codon 302. Conclusion: In contrast to the numerous reports, particularly from Asian countries, the selected HCC patients in this study show a very low mutation rate in the hot spot regions of the p53 gene. However, further mutation studies have to be conducted for the whole length of the p53 gene for more evaluation.
