Microbiology
Samaneh Rouhi; Rashid Ramazanzadeh
Volume 13, Issue 3 , July 2018, , Pages 348-356
Abstract
Background and Objective: Pseudomonas aeruginosa (P. aeruginosa) causes serious nosocomial and non-nosocomial infections. blaOxacillinases (OXA)-23 and blaOXA24/40 provide resistance to carbapenem antibiotics. The aim of this study was assessment of blaOXA-23 and blaOXA-24/40 in P. aeruginosa isolated ...
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Background and Objective: Pseudomonas aeruginosa (P. aeruginosa) causes serious nosocomial and non-nosocomial infections. blaOxacillinases (OXA)-23 and blaOXA24/40 provide resistance to carbapenem antibiotics. The aim of this study was assessment of blaOXA-23 and blaOXA-24/40 in P. aeruginosa isolated from patients with nosocomial and non-nosocomial infections. Methods: In this descriptive cross-sectional study performed in Sanandaj, Iran (Kurdistan province) in a period from December 2015 to August 2017, 146 isolates of Pseudomonas spp. were collected from patients’ specimens. Microbiological methods and polymerase chain reaction (PCR) with gyrB were applied for P. aeruginosa detection. Disk diffusion method with imipenem (IMP) (10µg) was performed for detection of resistant bacteria, and multiplex PCR of OXA-23 and OXA-24/40 were performed as well. Stata 12 with Fisher’s exact test and logistic regression were used for data analysis (P≤0.05).Results: PCR gyrB gene proved the existence of 91.78% P. aeruginosa isolates. Nosocomial infection with P. aeruginosa was observed in 41.79%. 27.61% of P. aeruginosa strains were resistant to IMP. blaOXA-23 and blaOXA24/40 were detected in 11.19% and 2.24% of the strains, respectively. 2.23% strains of P. aeruginosa showed a co-existence of blaOXA-23 and blaOXA24/40. There were no significant relationships between antibiotic resistance and presence of genes, and between IMP resistance and age, sex, city of residence, inpatient/outpatient, and specimen’s type (P≥0.05).Conclusion: Resistance to IMP and the presence of resistant genes in this study were observed in patients. More precautions should be taken in prescribing antibiotics and applying molecular techniques to detect genes, since they can cause antibiotic resistant.
GI, Liver & Pancreas Pathology
Chandan Kumar; Pragya Jain; Neelam Wadhwa; Preeti Diwaker; Nirupma Khan
Abstract
Mucormycosis is a rare but highly invasive opportunistic fungal infection. Gastrointestinal disease although uncommon is highly fatal. We report a case of jejunal mucormycosis in a 24 year old undernourished female with preceding surgical intervention for acute intestinal obstruction of tubercular etiology. ...
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Mucormycosis is a rare but highly invasive opportunistic fungal infection. Gastrointestinal disease although uncommon is highly fatal. We report a case of jejunal mucormycosis in a 24 year old undernourished female with preceding surgical intervention for acute intestinal obstruction of tubercular etiology. On 8th post-operative day, she developed oozing from suture line, prompting exploratory laparotomy, bowel resection, jejunostomy and ileal mucus fistula. Resected bowel showed one perforation and several areas of impending perforations. Characteristic broad, pauci-septate hyaline, empty looking hyphae with infrequent branching were found transmurally and showing angio-invasion. Local intestinal tissue trauma coupled with her sub-normal immune status permitted this unusual nosocomial infection. Histopathologic demonstration of the fungus in surgical specimens remains cornerstone of diagnosis of mucormycosis in view of its non-specific symptoms, low isolation rates of mycologic culture and lack of other rapid tests.
Alireza Nateghian; Gholamreza Irajian; Fatemeh Faraji
Volume 6, Issue 2 , April 2011, , Pages 79-85
Abstract
Background and Objectives: The aim of the study was to determine the role and characteristics of nosocomial and community acquired Staphylococcus sepsis in admitted children in tertiary centers in Iran. Patients and Methods: A cross sectional descriptive-analytic study was performed since March ...
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Background and Objectives: The aim of the study was to determine the role and characteristics of nosocomial and community acquired Staphylococcus sepsis in admitted children in tertiary centers in Iran. Patients and Methods: A cross sectional descriptive-analytic study was performed since March 2008 to March 2009 in which all blood cultures from various admitted patients were checked for Staphylococcu aurues in Aliasghar Children Hospital, Tehran, Iran. Upon diagnosis by appropriate microbiologic tests, antimicrobial testing was done according to CLSI methods. Results: Overall, 2647 blood culture samples from 5197 admitted children were sent from which, 25 cases of S. aurues septicemia were isolated; the rate was 4.8 in 1000 admissions;1.3 in 1000 admissions were nosocomial and 3.5 in 1000 admissions were community acquired sepsis. Ten cases were neonates and remainder was older. Eighteen cases were CA and 28% were NI septicemia with mean age of 38.8 months and 8.2 months, respectively. Mean duration of admission in NI group was 20.5 days, however it was 12.6 days in CA group; they also had higher mortality rate. Conclusion: The rate of Staphylococcus sepsis in this study was higher than developed countries for both CA and NI cases, both groups had high rate of resistance. Although most cases were CA in which significant proportion had underlying malignancy, NI group had a longer duration of admission and mortality.