Nafiseh Izadi; Mahboubeh Naderi Nasab; Elnaz Harifi Mood; Zahra Meshkat
Volume 9, Issue 3 , July 2014, , Pages 199-205
Abstract
Background & Objectives:Extended-spectrum-B-lactamase (ESBL)-producing strains of Klebsiella Pneumoniaare an important cause of many serious infections in hospitalized and nonhospitalized patients and delayed treatment of these infections in crease chance of death in patients. This study was performed ...
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Background & Objectives:Extended-spectrum-B-lactamase (ESBL)-producing strains of Klebsiella Pneumoniaare an important cause of many serious infections in hospitalized and nonhospitalized patients and delayed treatment of these infections in crease chance of death in patients. This study was performed to determine the prevalence of ESBL-producing K. Pneumonia and to evaluate the frequency of TEM and SHV genes among the clinical samples. Methods:One hundred and thirty isolates of K. Pneumoniawere collected at Imam Reza Hospital in Mashhad (Iran) from May 2011 to July 2012. ESBL production was determined by the double disk diffusion (DDs) test. PCR method was used to detect TEM and SHV genes. Results:Of 130 patients withK. pneumoniainfection 28 were out-patients and 102 hospitalized patients. The most specimens was urine samples (n=25 in out-patients, n=39 in hospitalized patients, totally 49.2%) followed by wound samples (n=3 in out-patients, n=21 in hospitalized patients, totally 21.5%), blood samples (n=19 in hospitalized, 14.6%). The prevalence of ESBL producingK. pnemoniaewas estimated 43% (n=56) including three of ESBLs positive isolates from out-patients and 53 from hospitalized patients. Of 56 ESBLs positive isolates, 44(87.54%) TEM, 39(69.64%) SHV and in 27 cases (48.21%) both TEM and SHV were detected. Conclusion:A high prevalence of ESBL-producing K. Pneumoniaamong the hospital isolates obtained of urinary followed by blood and wound samples were documented. The majority of them carried both TEM and SHV genes. Results of this study alarm for the physicians because treatment and control nosocomial infections for them were difficult.
Nasrin Shayanfar; Mitra Rezaei; Mehdi Ahmadi
Volume 5, Issue 1 , January 2010, , Pages 34-39
Abstract
Background and Objective: To evaluate extended spectrum betalactamase (ESBL) positive strains of Klebsiella pneumonia and Escherichia coli in positive bacterial cultures. Materials and Methods: In this analytical cross-sectional study, between March 2006 and March 2007, 170 bacterial isolates ...
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Background and Objective: To evaluate extended spectrum betalactamase (ESBL) positive strains of Klebsiella pneumonia and Escherichia coli in positive bacterial cultures. Materials and Methods: In this analytical cross-sectional study, between March 2006 and March 2007, 170 bacterial isolates including 133 cases of E. coli and 37cases of K. pneumonia were examined. All cases underwent double disk diffusion for ESBL. Demographic data were assessed and all data analyzed accordingly. Results: Patients’ mean age was 55±26.63 yr. Ninety six cases (56.5%) were female and 74 cases (43.5%) were male. Clinical presentation of infection were 118 cases UTI (96.4%), 15 cases septicemia (8.8%), 16cases wound infection (9.4%), 7 cases pneumonia (4.1%), 1 case meningitis (0.6%) and 13 cases other presentations (7.6%). Frequency of ESBL positive in E. coli isolates was 38 cases (28.6%) and in K. pneumonia isolates was 10 cases (27%). There was no significant correlation between ESBL positivity and age, gender, ward or clinical presentation of infection. Conclusion: Incidence of ESBL positive isolates of E. coli and K. pneumonia was high. These results should be considered in administration of broad-spectrum antibiotics by clinicians.
