Molecular Pathology
Freidoon Solhjoo; Akbar Safaie; Ahmad Monabati; Maral Mokhtari; Moeinadin Safavi
Volume 13, Issue 4 , October 2018, , Pages 438-446
Abstract
Background and Objective: Identification of cytogenetic and molecular changes plays an important role in acute myeloid leukemia (AML) patients. Thus, they are used in classification, prognosis and treatment of the disease. The CD123 expression and FLT3 gene mutations are also the variations that may ...
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Background and Objective: Identification of cytogenetic and molecular changes plays an important role in acute myeloid leukemia (AML) patients. Thus, they are used in classification, prognosis and treatment of the disease. The CD123 expression and FLT3 gene mutations are also the variations that may assist in prognosis and treatment of patients with AML.Methods: This study was performed on 76 patients as new cases of AML. The correlation between CD123 immunohistochemical (IHC) expression and FLT3 gene mutations with each other as well as morphological, immunophenotypical and cytogenetic factors was studied.Results: The results represented the CD123 IHC expression in 55.3% and FLT3 gene mutations in 28.9% of cases. We found that 81.3% of patients who had FLT3/ITD gene mutations revealed IHC of CD123 expression (P=0.019). The CD123 expression against FLT3 was also correlated with monocytic differentiation in bone marrow blasts (P=0.031). There were significant correlations between IHC expression of CD123 and FLT3/ITD mutations with a high percentage of aspirated bone marrow blasts (P=0.01 and P=0.006, respectively) as well as the lack of CD34 expression in bone marrow blasts (P=0.007 and P=0.021, respectively).Conclusion: The CD123 IHC positive AMLs were correlated with certain pathologic features, some of which can be similar with correlations of background mutation of FLT3/ITD; According to the negative predictive value (NPV), 88.2% of CD123 IHC showed FLT3 gene mutation. In addition to its use in targeted therapy, it could be a marker to decide what molecular tests to use in the next steps.
Ali Mohamad Malekasgar; Mohamad Esmaeil Motlagh; Mahmood Hashemi Tabar; Mohamad Ali Ghafari
Volume 1, Issue 3 , June 2006, , Pages 91-98
Abstract
Background and Objective: Pregnancy termination and recurrent abortion are one of the common complications during pregnancy and in patients with a bad obstetric history. Materials and Methods: In this study, a total of 154 individuals including 75 couples four single women from different communities ...
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Background and Objective: Pregnancy termination and recurrent abortion are one of the common complications during pregnancy and in patients with a bad obstetric history. Materials and Methods: In this study, a total of 154 individuals including 75 couples four single women from different communities and with various incomes were investigated chromosomal abnormalities using blood culture and chromosomal banding technique. Results: Chromosomal analysis of these patients revealed three abnormal karyotypes (3.8%) in three women and two abnormal karyotypes in conceptions. Two of these couples had consanguineous marriage and the remaining women included one isochromosome for X [46, x,I (xq)], two translocations [45, xx, t (15:21)] and [46, xx, t (7:14)], one trisomy ‘21’ (47, xx, +21), and a ring chromosome (46, xx, r(X). In addition, 27 conceptions had been reported for these five couples. These included 23 abortions with 18 of them within first trimester (78.26%) and four of them had abortions within second trimester (21.74%), one had a normal child, three had abnormal children, and one with stillbirth. Conclusion: It was found out that abnormal karyotype is present in 3.8% of patients with a bad obstetric history. There was also a close relationship between number of deliveries and abortions and this relation was statistically significant (p<0.01). In addition, consanguinity was also related with number of abnormal children (p<0.05). There was also a significant relationship between consanguinity and first trimester abortions (p<0.05). Therefore, in couples with more than three abortions, especially within first trimester, chromosomal evaluation can have a diagnostic value.