Sakineh Amouian; Mahdi Farzadnia; Bahram Memar; Armin Attaranzadeh; Naser Tayyebi
Volume 3, Issue 1 , January 2008, , Pages 25-29
Abstract
Background and Objective: Evaluation of tumor proliferative activity may provide a predicting parameter to estimate biologic aggression and a subsequent prognosis that has been evaluated in many malignancies. We have selected renal cell carcinoma (RCC) in this study. To determine tumor proliferative ...
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Background and Objective: Evaluation of tumor proliferative activity may provide a predicting parameter to estimate biologic aggression and a subsequent prognosis that has been evaluated in many malignancies. We have selected renal cell carcinoma (RCC) in this study. To determine tumor proliferative activity, KI67 antibody was applied and results were compared with apoptosis, applying P53 antibody and using immunohistochemical staining. Patients and Methods: Specimens of 30 patients who underwent radical nephrectomy for RCC were selected for histopathology and immunohistochemical study. Two different grading systems (S&H, Fuhrman) were used to calibrate average nucleoli diameter and tumor grading on all specimens. After processing of paraffin-embedded samples, they were immunohistochemically stained applying (MIB-1) KI67 monoclonal and P53 antibodies. Then, statistical analysis was done. Results: Tumor grading correlated with the average nucleus diameter. Positive reaction to KI67 and P53 antibodies in tumors increased as compared to control group. No significant relationship between age, sex and tumor grade was obtained. Conclusion: These two antibodies are as easy and reliable markers that could be applied on formalin-fixed tissues for better assessment of the biologic behavior of RCC and probably prediction of patients’ outcome.
Isa Jahanzad; Sakineh Amoueian; Armin Attaranzadeh
Volume 2, Issue 2 , April 2007, , Pages 71-73
Abstract
Introduction and Objective: Alport’s syndrome (hereditary nephritis with deafness) is a familial uncommon disease that ultra-structural studies are gold standard method of its diagnosis. Materials and Methods:We studied 26 Iranian patients suspicious of Alport’s syndrome by electron microscopy. ...
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Introduction and Objective: Alport’s syndrome (hereditary nephritis with deafness) is a familial uncommon disease that ultra-structural studies are gold standard method of its diagnosis. Materials and Methods:We studied 26 Iranian patients suspicious of Alport’s syndrome by electron microscopy. We examined 19 men and 7 women (male to female ratio was 2.7) and the average age was 1.5 years (with a range of 2.5-75 years). Results:The most common findings were irregularity of the glomerular basement membrane with splitting, lamination, and foot process effacement. Thinning in basement membrane was also reported and one case had disruption of capillary walls in study by only light microscopy. All of the patients had a diagnosis of megansial hypercellularity. Immunoflourscence studies did not show any deposits of immunoglobulin and complement component. Conclusion: definite diagnosis must be confirmed by ultra-structural findings.
