Alireza Azizzadeh Delshad; Mohammadreza Jalali Nadoushan; Ali Davati; Aida Rostami
Volume 11, Issue 3 , July 2016, , Pages 231-237
Abstract
Background: Nasal inflammatory disorders such as chronic rhinosinusitis and nasal polyp are among the most prevalent complications with high socioeconomic costs. Vascular Endothelial Growth Factor (VEGF) plays a key role in angiogenesis and cell proliferation. In the present study the effect of VEGF ...
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Background: Nasal inflammatory disorders such as chronic rhinosinusitis and nasal polyp are among the most prevalent complications with high socioeconomic costs. Vascular Endothelial Growth Factor (VEGF) plays a key role in angiogenesis and cell proliferation. In the present study the effect of VEGF on the development and prognosis of chronic rhinosinusitis and nasal polyp was investigated. Methods: This cross sectional study was performed on the nasal histological specimens of two groups of patients suffering from nasal polyp or chronic rhinosinusitis, and the expression of VEGF in the two groups was compared immunohistochemically. Based on the percentage of VEGF-positive cells the specimens were classified into four scores. Furthermore, the relations between the VEGF expression and some demographic characteristics were evaluated. Results: The VEGF immunohistochemistry findings indicated a significantly higher expression of VEGF in nasal polyp group compared to chronic rhinosinusitis without nasal polyp group. In terms of VEGF-expression scoring, in both groups most of the specimens were classified as score-2, namely indicating 10-50% of VEGF-positive epithelial cells. In both groups no significant relation between VEGF expression and age or sex of the patients could be seen. Conclusion: Local modulation of VEGF expression might be taken as a putative therapeutic strategy in management of sinunasal inflammatory disorders, especially nasal polyps.
Alireza Azizzadeh Delshad; Mohammad Hossein Ghaini; Marjan Heshmati
Volume 9, Issue 2 , April 2014, , Pages 124-132
Abstract
Background and Objective: The management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. In the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive ...
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Background and Objective: The management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. In the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line HT29 as an apoptosis resistant cell line, in vitro.
Materials and Methods: The HT29 cells were incubated with different concentrations of allicin (0-40µg/ml) and for different time periods (6-48h) to investigate its effect on cell proliferation and apoptotic cell death.
Results: Five and 10µg/ml allicin could induce a significant cell death only after 12h, whereas concentrations of 20 and 40µg/ml resulted in a significant cell loss as soon as 6h. The results of the TUNEL assay, presented as percentage of apoptotic cells to total cell loss, indicated that concentrations ≥5µg/ml significantly increased the apoptotic features in time periods 6-24h, but after 48h no significant changes could be detected. The ratio of the sum of the apoptotic features of the four studied time points to the total cell loss calculated after 48h was about 0.5.
Conclusion: Allicin can induce apoptosis in a concentration- and time-dependent manner with most considerable effects achieved at 24h and by concentrations higher than 10µg/ml.
Alireza Azizzadeh Delshad; Marjan Heshmati; Mohammad Hossein Ghaini
Volume 5, Issue 3 , June 2010, , Pages 126-131
Abstract
Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material ...
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Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material and Methods: Human colon adenocarcinoma cells HT29 were treated with different doses (1, 5 and 10 mg/ml) of fresh garlic extract in cell culture at 24, 48 and 72 h. Cell death was assessed by viability test, and its apoptotic nature was determined by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay. All experiments were statistically analyzed using SPSS 11.5 software Results: Garlic extract induced a dose-dependent cell death with the highest values at 72 hours. The apoptosis analyses by TUNEL assay demonstrated that in each dose/time group the apoptotic values are much less prominent than the related cell death percentage found in viability test. In every dose, the sum of apoptotic cells in all three-time points was nearly equal to the percentage of cell death at 72 hours. Conclusion: Fresh garlic extract can induce a dose-dependent apoptotic cell death in cultured HT29 cell line.
Alireza Azizzadeh Delshad; Marjan Heshmati; Taki Tiraihi
Volume 3, Issue 2 , March 2008, , Pages 67-74
Abstract
Background and Objective: As apoptotic cell death is extremely involved in physiological development and many pathological situations such as cancer and neurodegenerative diseases, the understanding of its molecular machinery can be useful in designing new therapeutic strategies. The present ...
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Background and Objective: As apoptotic cell death is extremely involved in physiological development and many pathological situations such as cancer and neurodegenerative diseases, the understanding of its molecular machinery can be useful in designing new therapeutic strategies. The present study investigated the temporal expression of the proapoptotic protein Bax in adult spinal motoneurons. Materials and Methods: Following unilateral mid-thigh sciatic transection in adult rats, the incidence and nature of spinal motoneuron loss were evaluated by means of light microscopic cell count and electron microscopy 1 day, 1 week, 1 month and 3 months post-operatively. In all groups the temporal expression of Bax was immunohistochemically determined and the findings were compared with the results of the cell count. Results: Following axotomy the related motoneurons underwent chromatolytic changes which increased up to one month and diminished in the 3-month group. One day following axotomy the number of motoneurons did not show any significant reduction, but thereafter a progressive cell loss occurred, which was most prominent after three months. Electron microscopic study confirmed the ultrastructural apoptotic nature of cell death. Bax immunohistochemistry indicated an increasing immunoreactivity up to one month post-axotomy, but in 3-month group it was clearly diminished. Conclusion: Following transection of a peripheral nerve in adult animals, related motoneurons undergo chromatolytic changes which in some neurons may proceed to apoptotic cell death. Although the proapoptotic protein Bax has long been believed as the main apoptotic factor, other Bax-independent pathways may also participate in the axotomy-induced neuronal apoptosis which must not be ignored.
Alireza Azizzadeh Delshad; Taki Tiraihi
Volume 2, Issue 3 , June 2007, , Pages 83-88
Abstract
Background and Objective: Evaluation of degenerative changes of motoneurons and their related synapses can be useful in understanding the mechanisms of neurodegenerative diseases and their potential treatment. The present electron microscopic and immunohistochemical study investigates the axotomy-induced ...
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Background and Objective: Evaluation of degenerative changes of motoneurons and their related synapses can be useful in understanding the mechanisms of neurodegenerative diseases and their potential treatment. The present electron microscopic and immunohistochemical study investigates the axotomy-induced changes of adult spinal motoneurons up to 3 months following sciatic nerve transection. Materials and Methods: Following unilateral mid-thigh sciatic transection in adult rats, the synaptophysin expression and ultrastructure of synapses in ventral horn of related spinal cord segments were studied 1 day, 1 week, 1 month, and 3 months postoperatively. In all groups the unaxotomized side of spinal cord was considered as control. The pattern of synaptophysin immunolabeling was classified into intact, partial, diffused, and negative types. Results: In 1 week and 1 month groups, the intact pattern decreased and the other 3 patterns increased, whereas 3 months postoperatively the patterns changed vice versa, i.e. intact pattern increased and the other 3 decreased. Electron microscopic studies indicated consistent ultrastructural changes such as synaptic vesicle displacement, synaptic membrane irregularity and synaptic stripping, which were most prominent after 1 month and declined in 3 month group. Conclusion: The present data indicate that following axon injury in adult motoneurons, synapses undergo obvious changes in ultrastructure and synaptophysin distribution, which increase up to 1 month postoperatively, and if the cell survives the insult the changes will attenuate and return to normal conditions thereafter.
