Hematopathology
Payam Azadeh; Nasser Rakhashni; Ali Yaghobi Joybari; Pegah Gorji Bayani; Samaneh Sarbaz; Maryam Farasatinasab
Volume 11, Issue 5 , October 2016, , Pages 439-442
Abstract
The oral cavity is uncommon site for metastatic disease usually discovered secondary to malignancy. We encountered with a rare case in which metastasis to mandibular bone was the first clinical sign in the diagnosis of breast cancer without any radiographic findings. A 49-yr-old premenopausal woman, ...
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The oral cavity is uncommon site for metastatic disease usually discovered secondary to malignancy. We encountered with a rare case in which metastasis to mandibular bone was the first clinical sign in the diagnosis of breast cancer without any radiographic findings. A 49-yr-old premenopausal woman, was referred to the Department of Medical Oncology of Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2014, presented with pain and tenderness in the left mandibular and temporal bone and paresthesia of the lower left lip and chin. CT scan of mandible showed no significant finding. Four months later, she was referred with complaints left breast pain for 4 wk and worsening swelling, pain and paresthesia. Breast examination revealed a 2 cm firm nodule on the left breast. Based on her medical history and histopathological study, metastatic carcinoma of the breast was suspected. She has received chemoradiotherapy that led to complete relief of her symptoms and remission of the disease. In the presence of an ambiguous sign in oral cavity such as jaw pain or paresthesia, diagnostic examination of malignancy is recommended.
Nasser Rakhshani; Mohammadreza Araste; Farid Imanzade; Mahshid Panahi; Fahimeh Safarnezhad Tameshkel; Masoud Reza Sohrabi; Mohammad Hadi Karbalaie Niya; Farhad Zamani
Volume 11, Issue 4 , October 2016, , Pages 409-415
Abstract
Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic ...
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Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections from suction rectal biopsies. Calretinin immunohistochemistry (IHC) may be a useful in its diagnosis. This study aimed to proof the usefulness of immunohistochemical staining for calretinin in rule out of Hirschsprung disease. Methods: Paraffin blocks and slides were retrieved from the pathology archives of Ali Asghar Hospital, Tehran, Iran from 2007 to 2011 with pathology report based on the presence (14 patients) or absence (70 patients) of ganglion cells and transitional zone anatomical region (10 patients). Slides were stained with hematoxylin and eosin method to confirm the initial diagnosis was verification again. After preparing the slides, they were stained by IHC for calretinin. Then, the results were analyzed using SPSS software. Results: In most patients, IHC for calretinin provided highly compatible results with hematoxylin-eosin findings in diagnosis of Hirschsprung disease. The values of specificity and accuracy between calretinin and standard histology (H&E) compared by the Fisher exact test declared calretinin presented significantly higher specificity and accuracy values than H&E staining (P <0.0001). Conclusion: Calretinin IHC overcomes most of the difficulties encountered using the histology hematoxylin-eosin. Then, IHC for calretinin is a good ancillary method used by pathologists in diagnosis of Hirschsprung disease.
Microbiology
Fahimeh Safarnezhad Tameshkel; Mohmmad Hadi Karbalaie Niya; Masuodreza Sohrabi; Mahshid Panahi; Farhad Zamani; Farid Imanzade; Nasser Rakhshani
Volume 11, Issue 3 , July 2016, , Pages 216-221
Abstract
Background: Nowadays, the immune response to hepatitis C (HCV) treatment has become a crucial issue mostly due to the interleukin 28B (IL-28B) polymorphism effects in chronic HCV patients. The aim of this study was to detect the polymorphism of IL-28B gene (rs12979860) in HCV genotype 1 patients treated ...
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Background: Nowadays, the immune response to hepatitis C (HCV) treatment has become a crucial issue mostly due to the interleukin 28B (IL-28B) polymorphism effects in chronic HCV patients. The aim of this study was to detect the polymorphism of IL-28B gene (rs12979860) in HCV genotype 1 patients treated with pegylated Interferon and Ribavirin. Methods: From the 2010 to 2012, a total of 115 peripheral blood mononuclear cells (PBMCs) of HCV patients who presented to Gastrointestinal & Liver Disease Research Center (GILDRC), Firoozgar Hospital, Tehran, Iran were enrolled in this retrospective cross sectional study. Samples were then categorized based on the presence of sustained virologic response (SVR and no-SVR). Variables including age, gender, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of the two groups were investigated based on different IL-28B genotypes. Results: Analysis by the variables of age and gender showed a mean age ± SD of 42.1±14.0 and gender variability of 44 females (38.2%) and 71 males (61.8%). Adding up these results, the analysis of ALT levels revealed that there was between 293 and 14 mg/ml; AST levels ranged between 217 and 17 mg/ml; the viral load (HCV RNA) ranged between 7,822,000 and 50 IU/ml; the prevalence of CC, CT and TT genotypes were 90.9%, 54% and 25.0%. Conclusion: IL-28B polymorphism has an effective impact on the therapeutic response to ribavirin and peginterferon combination therapy in chronic HCV patients infected by different genotypes. This polymorphism is crucial in natural clearance.
How to cite this article:
Safarnezhad Tameshkel F, Karbalaie Niya MH, Sohrabi M, Panahi M, Zamani F, Imanzade F, et al. Polymorphism of IL-28B Gene (rs12979860) in HCV Genotype 1 Patients Treated by Pegylated Interferon and Ribavirin. Iran J Pathol. 2016; 11(3):216-21.
Nasser Rakhshani; Roshanak Derakhshandeh; Seyed Amir Mirbagheri; Farhad Zamani; Ahad Atef Vahid; Mitra Mehrazma
Volume 6, Issue 3 , June 2011, , Pages 117-123
Abstract
Background and Objectives: Involvement of lymph nodes is an important prognostic factor in the most cancers, including colorectal cancer. In the recent years, invasion to blood and lymphatic vessels has been shown to predict involvement of lymph nodes and the number of involved nodes has been less studied ...
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Background and Objectives: Involvement of lymph nodes is an important prognostic factor in the most cancers, including colorectal cancer. In the recent years, invasion to blood and lymphatic vessels has been shown to predict involvement of lymph nodes and the number of involved nodes has been less studied issue. The aim of this study was determination of the relationship between the size of colorectal adenocarcinoma and lymph node involvement. Materials & Methods: In this cross-sectional study, 116 patients were enrolled with colorectal cancer from Rasoul-e-Akram and Mehr Hospitals in 2002-2008. Data analysis was performed by SPSS-15 software. Results were expressed as frequency, percent, and mean ±SD. We used Chi2, student t-test and correlation tests for statistical analysis. Results: 54.3% of patients were male and 45.7% were female. Mean age of them was 59.4± 12.9 years. Mean of tumor size (longest diameter) was 5.4± 2.2 (range: 1.5 to 12) cm. Mean number of involved lymph nodes was 4.9± 3.5(range: 1-14). There was no correlation between number of lymph node involvement and tumor size. There was no correlation between lymph node involvement and tumor and age group, sex, location and depth of tumor. Poorly differentiated tumor significantly correlated to lymph node involvement (P=0.001). Conclusion: There is no correlation between tumor size and number of involved lymph node in colorectal cancer. However, poor histopathologic grade is associated with lymph node involvement.
Nasser Rakhshani; Arman Morakabati; mohsen Ayati; mahshid hoormazdi
Volume 3, Issue 4 , September 2008, , Pages 225-228
Abstract
Endometriosis is the presence of benign endometrial glands and stroma outside the uterus. Urinary system involvement by endometriosis is a rare occurrence accounting for only 1.5 % of all endometriosis cases. Case 1. The patient was a 41 years old woman admitted for right flank pain. The physical ...
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Endometriosis is the presence of benign endometrial glands and stroma outside the uterus. Urinary system involvement by endometriosis is a rare occurrence accounting for only 1.5 % of all endometriosis cases. Case 1. The patient was a 41 years old woman admitted for right flank pain. The physical and gynaecologic examination was unyielding. Intravenous urography (IVU) revealed stenosis in distal part of right ureter, unfortunately associated with hydronephrosis. Case 2. A 26 years old woman who suffered from colic pain in low abdomen and pelvis. The only positive finding was microscopic hematuria. Abdominal sonography showed hydronephrosis of right kidney and proximal part of ureter. Ureterolysis by an open surgical procedure performed for both patients and histologic examination revealed endometriosis of ureter. Endometriosis of ureter is mainly asymptomatic and unfortunately ends in functionless kidney due to prolonged hydronephrosis. Early diagnosis needs high index of suspicion and intended use of paraclinic aids to save patients normal renal function. An individualized therapy plan depending on the patient's age and the extent of the endometriosis should be attempted.