Gynecologic Pathology
Maryam Kazemi Aghdam; Seyed Alireza Nadji; Azadeh Alvandimanesh; Maliheh Khoddami; Yassaman Khademi
Abstract
Background & Objective: Malignant breast tumors, which are one of the most important deadly cancers in women, like many other cancers, are proposed to be related to viruses etiologically. Proper management of breast carcinoma necessitates an identification of the etiological factors. Human Papilomavirus is ...
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Background & Objective: Malignant breast tumors, which are one of the most important deadly cancers in women, like many other cancers, are proposed to be related to viruses etiologically. Proper management of breast carcinoma necessitates an identification of the etiological factors. Human Papilomavirus is considered to have an etiological role in breast carcinoma. We carried out this study to find out if Human Papilomavirus-DNA is present in the malignant and benign breast tissue in our patients. Methods: Seventy five paraffin-embedded breast cancer tissues and 75 normal breast tissues and benign breast lesions were examined in this study (case-control) to look for Human Papilomavirus-DNA employing Nested Polymerase Chain reaction. The tissues were examined over a period of ten years in the pathology department of the Pathobiology Laboratory Center of Tehran. Result: No Human Papilomavirus-DNA was found in any of the malignant or control group specimens. Conclusion: Our results showed no evidence of Human Papilomavirus in cancerous and benign tissues, which is consistent with some other studies in English medical literature. More investigations using more specimens from different parts of the country are required to confirm the presence or absence of any connection between Human Papilomavirus and development of breast carcinoma in Iran.
Hematopathology
Maliheh Khoddami; Seyed Alireza Nadji; Paria Dehghanian
Abstract
Background and objective: Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferative disorder of unknown etiology and mainly affects young children. The histological feature is granuloma-like proliferation of langerhans-type dendritic cells. Although the possible role of viruses such ...
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Background and objective: Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferative disorder of unknown etiology and mainly affects young children. The histological feature is granuloma-like proliferation of langerhans-type dendritic cells. Although the possible role of viruses such as Epstein-Barr virus (EBV, Human Herpes virus -4), Human Herpes virus-6 (HHV-6), Herpes Simplex virus (HSV) types 1 and 2 and Cytomegalovirus (CMV, Human Herpes virus-5) is suggested in the pathogenesis of LCH by some investigators, its exact pathophysiology has not been cleared yet. In this study, we investigated the presence of HSV types 1 and 2 in Iranian children with LCH Methods: In this retrospective study, we investigated the prevalence of presence of HSV types 1 and 2 (in 30 patients with LCH), using paraffin-embedded tissue samples and 30 age and tissue-matched controls (operated for reasons other than infectious diseases) from the Department of Pediatric Pathology, Tehran, Iran, by nested Polymerase Chain reaction method. No ethical issues arose in the study, because only the pathology reports were reviewed and patients were anonymous. Results: We failed to find HSV types 1 and 2 DNA in any of the 30 patients with LCH or the control group. Conclusion: According to our findings, HSV types 1 and 2 do not appear to have any etiologic role in the pathogenesis of LCH in Iranian children. These results are in accordance with previous investigations with negative findings.
Maliheh Khoddami; yassaman Khademi; Maryam Kazemi Aghdam; Haleh Soltanghoraee
Volume 11, Issue 2 , April 2016, , Pages 120-126
Abstract
Background: Presence of discordance between the Gleason score on needle biopsy and the score of radical prostatectomy specimen is common and universal. In this study, we determined the accuracy of Gleason grading of biopsies in predicting histological grading of radical prostatectomy specimens and the ...
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Background: Presence of discordance between the Gleason score on needle biopsy and the score of radical prostatectomy specimen is common and universal. In this study, we determined the accuracy of Gleason grading of biopsies in predicting histological grading of radical prostatectomy specimens and the degree of overgrading and undergrading of prostatic adenocarcinoma in our center, which is one of the referral centers in Tehran. Methods: In this retrospective study, we analyzed the results of prostate needle biopsies and subsequent prostatectomies diagnosed at the Pathobiology Laboratory Center, Tehran, Iran in 45 patients between 2002 and 2013. Preoperative clinical data and theinformation from biopsy and prostatectomy specimens were collected.The accuracy, sensitivity, specificity, and positive and negative predictive values of different grades and groups were assessed. Pearson and Spearman correlation coefficient were used to determine the relation of different variables. Results: The biopsy Gleason score was identical to the scores in prostatectomy specimens in 68.2% cases, while 31.8% were discrepant by 1 or 2 Gleason score. We had 9.1% downgrading and 22.7% cases upgraded after prostatectomy. The sensitivity and positive predictive value was 86% and 79% for low grade, 67% and 75% for moderate grade, and 80% and 80% for high-grade tumors, respectively. Conclusion: Overall, the reliability of Gleason grading of needle biopsies in predicting final pathology was satisfavory. Moderate grade group was the most difficult to diagnose in needle biopsy.
Maliheh Khoddami; Parishad Ghavam
Volume 7, Issue 2 , April 2012, , Pages 112-120
Abstract
Background and objective: To determine the diagnostic accuracy and pitfalls of frozen section in ovarian tumors in one of the largest university affiliated gynecologic oncology centers in Tehran, and determine the cause of discrepancies.
Materials and Methods: We retrospectively analyzed the results ...
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Background and objective: To determine the diagnostic accuracy and pitfalls of frozen section in ovarian tumors in one of the largest university affiliated gynecologic oncology centers in Tehran, and determine the cause of discrepancies.
Materials and Methods: We retrospectively analyzed the results of frozen section and permanent diagnoses of ovarian masses by reviewing the reports in the department of Pathology of Imam Hussein Hospital from 1997 to 2009.
Results: Among 1498 cases of ovarian lesions, only 187 patients had both frozen and paraffin section diagnoses (age range 10-82 yr). 71.7% of these cases had complete concordance, 26.7% had partial and 1.6% had no concordance. The overall sensitivity and specificity of frozen section diagnosis were 100% and 99.3%, respectively. The sensitivity of frozen section diagnosisfor benign, borderline, and malignant lesions was 99.3%, 100% and 94.9%; and the specificities were 100%, 98.9% and 99.3% respectively.
Conclusion: Our results show high sensitivity and specificity of frozen section diagnosis in ovarian masses. Pathologist’s misinterpretation was the only cause of discrepancies.
Maliheh Khoddami; Maryam Barangi
Volume 7, Issue 2 , April 2012, , Pages 121-124
Abstract
Ovarian borderline serous tumors are uncommon. Combination of borderline serous adenofibromatous tumor and prominent micro papillary architecture is not previously reported. We report a case of borderline papillary serous adenofibromatous tumor (also called serous adenocarcinofibroma) with extensive ...
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Ovarian borderline serous tumors are uncommon. Combination of borderline serous adenofibromatous tumor and prominent micro papillary architecture is not previously reported. We report a case of borderline papillary serous adenofibromatous tumor (also called serous adenocarcinofibroma) with extensive micropapillary pattern in a 27 year-old married woman. She was infertile and presented with diffuse abdominal pain and dysparonia. Bilateral 5.3 and 4.5 cm solid ovarian masses were detected by sonography. Both masses were ovoid with tan-pink bosselated smooth external surfaces, and solid tan lobular cut surfaces. Microscopically, both tumors showed many papillary structures in a fibrotic stroma and contained multiple psammoma bodies. The papillae had broad hyalinized fibrotic stroma with many micropapillary projections arising from the main papilla, lined by mildly pleomorphic cuboidal cells. Mitotic activity was low with no marked nuclear atypia or stromal invasion. No extraovarian implants or metastases were identified.