Alireza Azizzadeh Delshad; Mohammad Hossein Ghaini; Marjan Heshmati
Volume 9, Issue 2 , April 2014, , Pages 124-132
Abstract
Background and Objective: The management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. In the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive ...
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Background and Objective: The management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. In the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line HT29 as an apoptosis resistant cell line, in vitro.
Materials and Methods: The HT29 cells were incubated with different concentrations of allicin (0-40µg/ml) and for different time periods (6-48h) to investigate its effect on cell proliferation and apoptotic cell death.
Results: Five and 10µg/ml allicin could induce a significant cell death only after 12h, whereas concentrations of 20 and 40µg/ml resulted in a significant cell loss as soon as 6h. The results of the TUNEL assay, presented as percentage of apoptotic cells to total cell loss, indicated that concentrations ≥5µg/ml significantly increased the apoptotic features in time periods 6-24h, but after 48h no significant changes could be detected. The ratio of the sum of the apoptotic features of the four studied time points to the total cell loss calculated after 48h was about 0.5.
Conclusion: Allicin can induce apoptosis in a concentration- and time-dependent manner with most considerable effects achieved at 24h and by concentrations higher than 10µg/ml.
Alireza Azizzadeh Delshad; Marjan Heshmati; Mohammad Hossein Ghaini
Volume 5, Issue 3 , June 2010, , Pages 126-131
Abstract
Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material ...
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Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material and Methods: Human colon adenocarcinoma cells HT29 were treated with different doses (1, 5 and 10 mg/ml) of fresh garlic extract in cell culture at 24, 48 and 72 h. Cell death was assessed by viability test, and its apoptotic nature was determined by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay. All experiments were statistically analyzed using SPSS 11.5 software Results: Garlic extract induced a dose-dependent cell death with the highest values at 72 hours. The apoptosis analyses by TUNEL assay demonstrated that in each dose/time group the apoptotic values are much less prominent than the related cell death percentage found in viability test. In every dose, the sum of apoptotic cells in all three-time points was nearly equal to the percentage of cell death at 72 hours. Conclusion: Fresh garlic extract can induce a dose-dependent apoptotic cell death in cultured HT29 cell line.
