Microbiology
Sina Neshat; Padideh Daneii; Negar Neshat; Sina Raeisi; Saba Raeisi; Seyed Mohammad Malakooti; Noushin Afshar Moghadam
Abstract
Human papillomavirus infection is one of the most common genital infections. More than 100 types of the virus have been identified, most of which can infect the genital mucosa. The virus is associated with cancerous and precancerous lesions of the cervix. Some types, such as human papillomavirus 16 and ...
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Human papillomavirus infection is one of the most common genital infections. More than 100 types of the virus have been identified, most of which can infect the genital mucosa. The virus is associated with cancerous and precancerous lesions of the cervix. Some types, such as human papillomavirus 16 and 18, are highly carcinogenic; some other types, such as human papillomavirus 6 and 11, are mildly carcinogenic, with HPV 31.33 in between. This study describes the relationship between different types of HPV infection and the findings of a Pap smear. In this prospective study, 1,500 samples from patients who admitted to a private Pathology Laboratory in Isfahan were collected during the years 2019-2020. Two samples were collected from each patient, one for Pap smear study and the other for polymerase chain reaction (PCR) test to detect different types of human papillomavirus (HPV) infection. In a study of 1,500 samples, 236 were positive for Papillomavirus (HPV) infection in Pap smear. By examining the genotype of positive examples, it was found that 14.8% of the samples were infected with type 16, and 1.7% were infected with type 82. HPV infection is common in Iran and is almost similar to European countries such as Germany and Spain. We also found that using a polymerase chain reaction (PCR) method to detect HPV viruses in vaginal secretions could be very useful. Our findings also show which disease is most associated with each type of HPV.
Noushin Afshar Moghaddam; Parvin Mahsuni; Diana Taheri
Abstract
Background and Objectives: Angiogenesis is essential for growth and metastasis of solid malignancies. Tumor vessel count and expression of vascular endothelial growth factor (VEGF), a potent angiogenic factor, have been associated with prognosis. This study was designed to assess vessels density by using ...
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Background and Objectives: Angiogenesis is essential for growth and metastasis of solid malignancies. Tumor vessel count and expression of vascular endothelial growth factor (VEGF), a potent angiogenic factor, have been associated with prognosis. This study was designed to assess vessels density by using CD31 and CD105 (Endoglin) and their correlation with expression of VEGF and proliferative index (Ki67) in Glioblastoma multiforme (GBM). Methods: We examined these parameters in GBM specimens from 50 adult patients; referred to AlZahra hospital Pathology Lab between 2001 to 2006.These patients did not receive pre-operative therapy. Paraffin-embedded tumor specimens were immunohistochemically stained for CD31, CD105 (Endoglin), VEGF and Ki67 (proliferation index) monoclonal antibodies. Microvessel density (MVD) was evaluated by immunostaining for CD31 and CD105.Then the results were compared between the two and also with VEGF receptors and Ki67 index. Results: CD105-MVD was significantly higher in Glioblastoma compared with peritumoral normal (14.28 vs. 6.68: P=0.012). We did not find such difference for CD31. The mean of CD105-MVD was significantly higher than CD31-MVD in Glioblastoma tissue (P<0.001) although there was a significant positive relationship between them (Pearson’s r=0.630 P<0.001).The VEGF scoring for tumoral tissue was 12 % (score:1), 46% (score:2) and 42% (score:3).For peritumoral normal tissue were 92% (score:1) and 8% (score:2) . So they reach to statistical significance (Chi Square, P= 001). Both MVD of CD105 and CD31 have significant relationship with VEGF (P<0.001). Conclusion: We suggest that Endoglin can be used as a specific and sensitive marker for evaluation of angiogenesis in Glioblastoma.
Noushin Afshar Moghaddam; Parvin Mahsoni; Amir Pooyan Tabibi
Volume 3, Issue 2 , March 2008, , Pages 75-80
Abstract
Objective: To review Her-2/neu and Tp53 status and their correlation with all other prognostic clinicopathologic features of infiltrative ductal breast carcinomas. Materials and Methods: This cross sectional study was performed on 139 patients with infiltrative ductal breast carcinoma who were ...
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Objective: To review Her-2/neu and Tp53 status and their correlation with all other prognostic clinicopathologic features of infiltrative ductal breast carcinomas. Materials and Methods: This cross sectional study was performed on 139 patients with infiltrative ductal breast carcinoma who were diagnosed between May 2000 and March 2006 at the surgery and pathology departments of Alzahra Hospital, Isfahan, Iran. Immunostaining (IHC) for Tp53 and Her2/neu were performed on formalin-fixed, paraffin-embedded tissues based on an avidin-biotin-peroxidase complex technique. The relationship of these markers with clinicpathologic parameters including age, axillary lymph nodes status, tumor size and histological grade were evaluated. Results: It was found out that Her2/neu-positive cases were greater among metastatic lymph nodes than in patients without metastasis, however it was not significant (p=0.1). A significant association was also observed between Her2/neu status and tumor grading (p=0.01). On the contrary, no association was found with other clinicpathologic parameters. In this study, Tp53 presentation in high-grade carcinomas was significantly more as compared to low grade ones (p=0.03). A significant association was also observed between Tp53 and tumor size (p =0.01). There was no association with menopausal status and lymph node status. Conclusion: IHC determined that Her-2/neu and Tp53 expressions are not associated with nodal and menopause status. Conversely, a correlation was found between Her-2/neu, Tp53 expressions and high histological grade of tumor. However, to validate these findings, long-term prospective studies on patients’ survival are necessary.
Noushin Afshar Moghaddam; Diana Taheri; Azar Naimi; Faegheh Taghizadeh; Shahram Taheri
Volume 2, Issue 2 , April 2007, , Pages 77-79
Abstract
A 54 year old man with history of cardiomyopathy and chronic fibrillation first presented with bloody diarrhea. Then during the assessment of his abnormal lab. data revealed cast nephropathy in renal biopsy, gamma peak in protein electrophoresis and fine lytic lesions in skull graphy. His bone marrow ...
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A 54 year old man with history of cardiomyopathy and chronic fibrillation first presented with bloody diarrhea. Then during the assessment of his abnormal lab. data revealed cast nephropathy in renal biopsy, gamma peak in protein electrophoresis and fine lytic lesions in skull graphy. His bone marrow aspiration showed multiple myeloma and blood smear revealed plasma cell leukemia. Our patient was diagnosed to have multiple myeloma and plasma cell leukemia Plasma cell leukemia is a rare form of plasma cell dyscrasia.The second type evolves as a terminal event in some of the patients with multiple myeloma. PCL and multiple myeloma simultaneously recognizes in this case(very rare event).
