Molecular Pathology
Amirhossein Jafarian; Masoumeh Jafaripour; Masoumeh Gharib; Maryam Salehi; Nema Mohamadian Roshan; Sare Etemad; Khatoone Mirshekar; Maryam Sheikhi; Masoumeh Heidari; Behnaz Ahmadian; Zahra Khoshnegah; Hossein Ayatollahi; Payam Siyadat
Abstract
Background & Objective: Epithelial ovarian cancer (EOC) is the most prevalent type of ovarian cancer. Previous studies have elucidated different pathways for the progress of this malignancy. The mutation in the B-Raf proto-oncogene, serine/threonine kinase (BRAF) gene, a member of the MAPK/ERK signaling ...
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Background & Objective: Epithelial ovarian cancer (EOC) is the most prevalent type of ovarian cancer. Previous studies have elucidated different pathways for the progress of this malignancy. The mutation in the B-Raf proto-oncogene, serine/threonine kinase (BRAF) gene, a member of the MAPK/ERK signaling pathway, plays a role in EOC. The current study aimed to determine the frequency of the BRAF V600E mutation in ovarian serous and mucinous tumors, including borderline and carcinoma subtypes.Methods: A total of 57 formalin-fixed paraffin-embedded samples, including serous borderline tumors (SBTs), low-grade serous carcinomas (LGSCs), high-grade serous carcinomas (HGSCs), mucinous borderline tumors (MBTs), and mucinous carcinomas, and 57 normal ovarian tissues were collected. The BRAF V600E mutation was analyzed using polymerase chain reaction (PCR) and sequencing.Results: While 40% of the SBT harbor BRAF mutation, we found no BRAF mutation in the invasive serous carcinoma (P=0.017). Also, there was only 1 BRAF mutation in MBT and no mutation in mucinous carcinomas. In addition, we found no mutation in the control group.Conclusion: The BRAF mutation is most frequent in borderline tumors but not in invasive serous carcinomas. It seems that 2 different pathways exist for the development of ovarian epithelial neoplasms: one for borderline tumors and the other for high-grade invasive carcinomas. Our study supports this hypothesis. The BRAF mutation is rare in mucinous neoplasms.
Hematopathology
ehsan yazdandoust; mohammad hadidi sadeghian; seyyede fatemeh shams; Yasaman Saadatpour; payam siyadat; maryam sheikhi; Monavar Afzal Aghaee; Hossein Ayatollahi
Abstract
Background & Objective: Acute myeloid leukemia (AML) is a hematopoietic malignancy caused by genetic abnormalities. These days, molecular and genetic factors are usually used as diagnostic and prognostic markers. FLT-3 is one of the most known diagnostic factors in AML. MDR1 gene belongs to the ATP ...
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Background & Objective: Acute myeloid leukemia (AML) is a hematopoietic malignancy caused by genetic abnormalities. These days, molecular and genetic factors are usually used as diagnostic and prognostic markers. FLT-3 is one of the most known diagnostic factors in AML. MDR1 gene belongs to the ATP binding cassette family; it is known as one of the chemotherapy-resistant causes of AML. We aimed to study FLT-3ITD mutations and their association with MDR1 gene expression in AML individuals.Methods: For investigation, 80 AML individuals and 20 healthy controls were selected. This study was done in the cancer molecular pathology research center of Mashhad University of Medical Sciences (MUMS), Iran during 2017-2019. FLT3-ITD mutation was assessed by polymerase chain reaction (PCR); Real-time quantitative PCR was performed to measure the amount of MDR1 gene expression. Bone marrow and blood smears of patients were evaluated in terms of morphology. SPSS 16.0 was used for data analysis.Results: FLT3-ITD mutation and MDR1 overexpression were found in 18.8% and 23.8% of AML patients, respectively. Statistical analysis did not show any relations or association between these two markers. Cuplike morphology was observed in blast cells in 21.25% of AML cases, which was associated with FLT3-ITD mutation presence.Conclusion: FLT-3 and MDR1 do not affect each other. It is suggested to perform survival studies to determine the exact role of MDR1 overexpression in drug resistance issues.
Cytology
Narjes Soltani; Farzaneh Mirzaei; Hossein Ayatollahi
Abstract
Background & Objective: One of the major genetic causes of recurrent spontaneous abortions is parental chromosomal abnormalities. The objectives of the study were to determine, compare and analyze the incidence and distribution of chromosomal abnormalities in couples with recurrent miscarriages from ...
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Background & Objective: One of the major genetic causes of recurrent spontaneous abortions is parental chromosomal abnormalities. The objectives of the study were to determine, compare and analyze the incidence and distribution of chromosomal abnormalities in couples with recurrent miscarriages from Northeastern Iran.Methods: This study was conducted at Ghaem Hospital, Mashhad, Iran. We evaluated karyotype results of 608 couples with history of recurrent spontaneous abortion. The standard method was used for culturing peripheral venous blood lymphocytes.Results: Chromosome aberrations were detected in 43 patients (3.54%), including 25 females and 18 males. Structural chromosomal abnormality was detected in 40 cases, including balanced translocations (25 cases), robertsonian translocations (4 cases), inversions (10 cases) and numerical chromosome aberrations (3 cases). Polymorphic variants were observed in 22 individuals.Conclusion: The frequency of chromosomal abnormalities in couples with Recurrent Spontaneous Abortion (RSA) in our study is 3.54%. Reciprocal translocation, pericentric inversions, robertsonian translocations, and numerical abnormality observed among couples who had experienced recurrent spontaneous abortions and that these couples might benefit from cytogenetic analysis.
Biology & Genetic
Narjes Soltani; Farzaneh Mirzaei; Hossein Ayatollahi
Abstract
Background & Objective: Primary amenorrhea refers to the absence of menstruation in females of reproductive by age 16 when the development of secondary sexual characteristics is evident (breast development, pubic hair) or by age 14 when there are no secondary sexual characteristics are present. Primary ...
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Background & Objective: Primary amenorrhea refers to the absence of menstruation in females of reproductive by age 16 when the development of secondary sexual characteristics is evident (breast development, pubic hair) or by age 14 when there are no secondary sexual characteristics are present. Primary amenorrhea can occur in several quite different reasons. Common hormonal causes of primary amenorrhea include constitutional delay, hypothalamic or pituitary disorders, chronic systemic disease, and primary ovarian insufficiency, some endocrine gland disorders, and other causes. Previous studies suggested that chromosomal abnormality is the second most common cause of amenorrhea. This report aims to measure the prevalence of the chromosomal abnormality in primary amenorrhea (PA) patients in the northeast of Iran.Methods: Chromosomal study was carried out on 200 patients with clinical features. The standard method for culturing peripheral venous blood lymphocyte was to prepare metaphase chromosomes and perform routine GTG band analysis.Results: The results revealed that 71% of PA had normal female karyotype (46,XX) and 29% showed different chromosomal abnormalities. The chromosomal abnormalities can be categorized into seven primary groups with or without mosaicism. 1- The most common karyotype was X chromosome aneuploidy (10.5%, n=21), 2- Male karyotype with or without structural abnormality of Y chromosome (5.5 %, n=11), 3- Mosaicism of turner karyotype and structural anomalies of X chromosome (4%, n=8), 4- Structural anomalies of the X chromosome (3.5%, n=7), 5- Mosaicism of turner karyotype and normal karyotype (3%, n=6), 6- Mosaicism of turner karyotype and male karyotype (1.5%, n=3) and 7- Super female karyotype (1%, n=2).Conclusion: The present study has emphasized that early cytogenetic and timely investigation can be necessary for the evaluation of primary amenorrhea.
Hematopathology
Hossein Ayatollahi; Ali Bazi; Mohammad Hadi Sadeghian; Ali Fani; Payam Siyadat; Maryam Sheikhi; Omolbanin Sargazi-aval
Abstract
Background & Objective: Acute promyelocytic leukemia (APL) with t(15;17)(q22;q12) is a relatively common subtype of acute myeloid leukemia (AML). Here, our objective was to ascertain the survival of patients with this leukemia in north-east of Iran. Methods: Survival rates of ...
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Background & Objective: Acute promyelocytic leukemia (APL) with t(15;17)(q22;q12) is a relatively common subtype of acute myeloid leukemia (AML). Here, our objective was to ascertain the survival of patients with this leukemia in north-east of Iran. Methods: Survival rates of 42 APL patients with t(15;17)(q22;q12) were assessed. Clinical information was obtained from archived medical records. Statistical analysis was performed by SPSS 18 software using log-ranked test and Kaplan Maier survival analysis. Results: Females and males comprised 49% and 51%, respectively. The mean age at diagnosis was 34.3 ± 14.1 years old. During the study period, 17 demises occurred in males, while this number was 7 in females. The mean survival of patients (month) was 23.22 ± 3.57 (95% CI: 16.21 ± 30.2). The five-year survival rate obtained 30%. Regarding demographic and clinical features, the highest rates of 5-year survival were recorded in patients with 20-35 years old (47.6%), males (51%), white blood cell count <10±/l (48%), and platelet count >140 ± /l (100%). Conclusion: Younger age, lower WBC count and higher platelet count were significantly associated with longer survival in AML patients with t(15;17)(q22; q12).
Molecular Pathology
Amir Hossein Jafarian; Nema Mohammadian Roshan; Hossein Ayatollahi; Abbas Ali Omidi; Masoumeh Ghaznavi; Masoumeh Gharib
Abstract
Background & Objective:Idiopathic pulmonary fibrosis (IPF) is a chronic and uniformly fatal interstitial lung disease with incompletely understood pathogenesis. Several studies have given the evidence for and against viral cofactors in the pathogenesis of Idiopathic pulmonary fibrosis. In this study ...
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Background & Objective:Idiopathic pulmonary fibrosis (IPF) is a chronic and uniformly fatal interstitial lung disease with incompletely understood pathogenesis. Several studies have given the evidence for and against viral cofactors in the pathogenesis of Idiopathic pulmonary fibrosis. In this study Epstein-Bar Virus (EBV) and Human Herpesvirus 8 (HHV-8) have been studied for a possible role in the pathogenesis of IPF.Methods:Polymerase chain reaction (PCR) was employed for the detection of EBV and HHV-8 in 58 formalin-fixed paraffin-embedded lung tissue specimens (29 controls and 29 IPF specimens).Results:EBV DNA was present in the lung tissue of 6 out of 29 (20.7%) IPF specimens compared with 1 out of 29 (3.4%) controls (P=0.102). The HHV-8 gene was identified in 3 out of 29 (10.3%) cases of IPF specimens. The control group showed no evidence of HHV-8 gene (P=0.227).Conclusion:Although multiple studies are strongly suggestive of a role for EBV and HHV-8 in the development of IPF, there was no statistically significant difference in the prevalence of EBV and HHV-8 DNA in the IPF specimens and controls in this study.
Molecular Pathology
Amir Hossein Jafarian; Nema Mohammadian Roshan; Masoumeh Gharib; Vahid Moshirahmadi; Aida Tasbandi; Amir Ali Ayatollahi; Hossein Ayatollahi
Abstract
Background and Objective: The primary goal of this study is to develop a rigorous understanding ofthe correlation between COX-2 expression and malignant melanoma prognostic factors. Material and Methods: In this cross-sectional study, we analyzed 60 cases of cutaneous malignant melanoma. The related ...
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Background and Objective: The primary goal of this study is to develop a rigorous understanding ofthe correlation between COX-2 expression and malignant melanoma prognostic factors. Material and Methods: In this cross-sectional study, we analyzed 60 cases of cutaneous malignant melanoma. The related stained slides were reviewed by two pathologists. The results were interpreted according to the COX2 staining index (SI), tumor thickness (Breslow, Clark), number of mitoses per 10 hpf, and melanoma types. Gender, lymph node involvement, metastasis, and survival were considered as evaluation factors as well. Results: The expression of the COX-2 protein was evident in 98.4% of cases. A strong Staining Index(SI) was reported in 60% of all melanomas, moderate staining was detected in 20.8% and weak staining in 10%; 1.6% of studied cases showed no staining. Benign nevus specimens showed no staining for the COX-2 enzyme. Conclusion: We have demonstrated that COX-2 is strongly expressed in the majority of malignant melanomas and that the SI score of COX-2 is related to the number of mitoses, tumor thickness (based on Clark level and Breslow), melanoma sub-type, lymph node involvement, and metastases; No association was noted between the anatomic site, gender, and survival. COX-2 can be applied as a prognostic factor in malignant melanoma and a promising candidate for future target therapies.
Hematopathology
Seyyede Fatemeh Shams; Hossein Ayatollahi; Mohammad hadi Sadeghian; Monavar Afzal Aghaee; Sepideh Shakeri; Ehsan Yazdandoust; Maryam Sheikhi; Nafiseh Amini; Samane Bakhshi; Afsane Bahrami
Volume 13, Issue 4 , October 2018, , Pages 397-402
Abstract
Background and Objective: Janus kinase 2 (JAK2) and Myeloproliferative Leukemia (MPL) mutations are confirmatory indicators for Myeloproliferative Neoplasm (MPN). The current study was performed to determine the frequency of MPL mutation in MPN patients without JAK2 mutation, in order to assign MPL mutation ...
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Background and Objective: Janus kinase 2 (JAK2) and Myeloproliferative Leukemia (MPL) mutations are confirmatory indicators for Myeloproliferative Neoplasm (MPN). The current study was performed to determine the frequency of MPL mutation in MPN patients without JAK2 mutation, in order to assign MPL mutation frequency in North-East of Iran.Methods: Total of 105 negative JAK2 cases including 5 Myeloproliferative Disorders (MPD), 15 Polycytemia Vera (PV) and 15 Essential Thrombocytosis (ET) who referred to Qaem Medical Center were assigned to this study. ARMS-PCR was carried out for measuring MPL mutations. Results: A significant difference was observed between MPL mutant and non-mutant groups from overview of MPL mutation (P=0.00001). From the total studied population, 14.28% were ET cases and 4.71% of them had splenomegaly. About 66.66% had thrombocytosis and 33.33% of all the individuals had leukocytosis according to WHO criteria, and 4.76% of non-MPL mutant individuals had splenomegaly (P=1). This mutation was reported in 4-6% of ET and PMF individuals. In this research, 4.76 % of studied individuals had MPL (W515L/K) mutation, which were diagnosed with ET.Conclusion: Generally, the presence of JAK2 and MPL mutations are the most important criteria for MPN diagnosis. The obtained frequency of MPL mutation was similar to previous studies. Despite the high frequency of JAK2 and Philadelphia abnormality, MPL mutation was rare in myeloprolifrative disorders. Further studies are suggested to investigate its prognostic effects for these diseases.
Diagnostic Pathology
Amir Hossein Jafarian; Khatoone Mirshekar; Sare Etemad; Masoumeh Jafaripour; Mansoore Darijani; Maryam Sheikhi; Hossein Ayatollahi; Sepideh Shakeri; Seyyede Fatemeh Shams; Saeed Davari
Volume 13, Issue 4 , October 2018, , Pages 415-421
Abstract
Background and Objective: BRAF mutations were studied in various populations for prostate carcinoma (PC); however, mutations in BRAF gene are unusual compared to KRAS. Oncogenic activating of BRAF mutations were studied lately in almost 0%-10% of prostate cancer cases. Methods: In this retrospective ...
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Background and Objective: BRAF mutations were studied in various populations for prostate carcinoma (PC); however, mutations in BRAF gene are unusual compared to KRAS. Oncogenic activating of BRAF mutations were studied lately in almost 0%-10% of prostate cancer cases. Methods: In this retrospective study, we gathered 100 formalin-fixed paraffin-embedded samples of prostate adenocarcinoma. A hundred archived samples of adjacent benign prostatic hyperplasia were chosen as normal control. This study was done in pathology laboratory of Qaem Hospital during 2013-2015.Results: Total number of 200 PC and normal cases was investigated for BRAF V600E mutation. The BRAF V600E mutation was found in only 4 patients but it was not detected in normal cases. There were no significant differences between patient and control groups for this mutation (P>0.99). The frequency of BRAF V600E mutation was not significant in different age groups (P>0.285); the most frequency was related to the age range of 71-80. No significant difference was observed between tumor grade and BRAF mutation (P=0.21).Conclusion: According to our findings, BRAF gene mutations did not play essential role in PC. Therefore, anti-BRAF (V600E) could not be considered as a proper target for therapy.
Biology & Genetic
Hossein Ayatollahi; Alireza Tavassoli; Amir Hossein Jafarian; Amin Alavi; Sepideh Shakeri; Seyyede Fatemeh Shams; Maryam Sheikhi; Neda Motamedi Rad; Mohammadhadi Sadeghian; Afsane Bahrami
Abstract
Background & objective: KRAS mutations are reported in many types of cancers including pancreas, lung, colon, breast, and gastric (GC). High frequency of KRAS mutation is observed in the pancreas, colon, and lung cancers; they commonly arise in codon 12 and 13 of exon 2. Due to the lack of information ...
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Background & objective: KRAS mutations are reported in many types of cancers including pancreas, lung, colon, breast, and gastric (GC). High frequency of KRAS mutation is observed in the pancreas, colon, and lung cancers; they commonly arise in codon 12 and 13 of exon 2. Due to the lack of information about the frequency of KRAS mutations in the Northeast of Iran, the current study aimed at evaluating KRAS frequency in cases with GC in this region. Methods: A total of120 formalin-fixed, paraffin-embedded blocks of patients with GC were assessed. The assays to detect KRAS in codon 12 and 13 were obtained through the peptide nucleic acid (PNA)-clamp. Results: Totally 87 male and 33 female patients were analyzed in the current study. The mean age of the subjects was 55 years. The most common tumoral fragment was located on the body with 48 cases (40%) and the less frequent was related to fondues with six cases (5%). Of the 120 GC samples, 16 (13.3%) cases had codon 12 KRAS mutation, and 16.7% had codon 13 mutations. There were no significant relationships between gender, age, and KRAS mutations in the studied specimens. Conclusion: In conclusion, the overall frequency of KRAS codon 12 and 13 mutations in GC was 30% in the current study population. Frequency of KRAS codon 12 and 13 mutations had significant correlation with tumors location. Different pathogenic mechanisms are suggested for GC according to tumor location. The current study results may be an important diagnostic tool for physicians managing atrophic gastritis.
Biology & Genetic
Meysam Rezaeishahmirzadi; Neda Motamedi Rad; Mehdi Kalantar; Hossein Ayatollahi; Sepideh shakeri; Maryam Sheikhi; Mohammad Shekari
Abstract
Background and Objective: The current study aimed at assessing the relationship between gastritis and peptic ulcer susceptibility and inflammation-related gene polymorphisms in Iranian patients. Gastritis and peptic ulcer are common medical complications with serious outcomes on the quality of life. ...
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Background and Objective: The current study aimed at assessing the relationship between gastritis and peptic ulcer susceptibility and inflammation-related gene polymorphisms in Iranian patients. Gastritis and peptic ulcer are common medical complications with serious outcomes on the quality of life. Inflammatory responses of gastric mucosa are associated with helicobacter pylori, but most infected patients remain asymptomatic. There is strong evidence that inflammatory response is a major part of its etiology. Methods: The current case-control study aimed at examining genetic polymorphisms in inflammatory cytokines interleukin (IL)-4, and IL-10 using polymerase chain reaction-variable number tandem reaped (PCR-VNTR) and PCR-restriction fragment length polymorphism (RFLP) methods, respectively in 603 genotyped patients admitted to Mohammadi Hospital in Bandar Abbas, Iran (198 patients with gastritis, 84 with peptic ulcer, and 321 patients as controls). Results: No significant associations were detected in genotype and allele frequencies of IL-10 and IL-4 between the case (with gastritis and peptic ulcer) and control groups. Conclusion: In conclusion, the results of the analyses suggested that these polymorphisms may not predispose the carriers to gastritis and peptic ulcer development.
Hematopathology
Hossein Ayatollahi; Azar Fani; Ehsan Ghayoor Karimiani; Fateme Homaee; Arezoo Shajiei; Maryam Sheikh; Sepideh Shakeri; Seyyede Fatemeh Shams
Abstract
Background and objective: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ Hybridization ...
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Background and objective: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ Hybridization (CISH) to assess HER2 status of biopsy specimens. Methods: To elucidate the status of HER2 gene amplification, biopsies of breast carcinoma from 120 patients with 2+ IHC status were analyzed by qPCR and CISH. Results: The results of the two experiments were compared, and it was depicted that the concordance rate between CISH and qPCR assays was 88.1%.The quantification of HER2 gene with CISH and qPCR showed that there was a significant correlation (p value= 0.0001 and r= 0.808). Conclusion: The results of this research support the idea that qPCR is a precise and reproducible technique, which can be employed as a supplementary method to evaluate HER2 status.
Hematopathology
Fatemeh Homaei Shandiz; Azar Fani; Sepideh Shakeri; Maryam Sheikhi; Abouzar Ramezani Farkhani; Arezoo Shajiei; Hossein Ayatollahi
Abstract
Background:Breast cancer remains the most common and second lethal cancer in females. HER-2/neu is one of the most important amplified oncogene in breast cancer. The amplification of HER-2 is correlated with decreased survival, metastasis, and early recurrence. The amplification of HER-2/neu gene ...
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Background:Breast cancer remains the most common and second lethal cancer in females. HER-2/neu is one of the most important amplified oncogene in breast cancer. The amplification of HER-2 is correlated with decreased survival, metastasis, and early recurrence. The amplification of HER-2/neu gene and synthesis of the protein are reported in 10%-34% of breast cancer cases associated with tumor size, advanced tumor stage, high-grade tumor, young age at diagnosis, absence of steroid hormone receptor, and lymph node involvement. Methods: Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) methods are options to evaluate HER-2 expression. The current study aimed at identifying the correlation between FISH and real-time polymerase chain reaction (PCR) in measuring HER-2 expression. Results: The study investigated the performance of the real-time PCR as measured against FISH method in IHC +2 borderline cases. In a total of 120 IHC 2+ samples, 58.3% were negative and 41.6% positive for HER-2 gene, confirmed by FISH as a gold standard method. The real-time PCR ratio was HER-2 gene by FISH as a gold standard assay. Conclusion: Despite the fact that real-time PCR is a promising method to evaluate HER-2 over expression and a supplementary array to FISH, according to the results of the present study it cannot be utilized instead of gold standard techniques; therefore, additional studies should be carried out to appraise the value of this method to evaluate HER-2 over expression.
Mohammad Reza Keramati; Mohammad Hadi Sadeghian; Hossein Ayatollahi; Houshang Rafatpanah; Mohammad Khajeh Daluei; Nafise Baesi
Volume 6, Issue 2 , April 2011, , Pages 56-62
Abstract
Background and Objectives: Complement proteins are some of the most important plasma proteins of the innate immune system. Impaired immune function is reported in subjects who are iron deficient, and there are documents that these patients are prone to infection. This study was conducted to show whether ...
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Background and Objectives: Complement proteins are some of the most important plasma proteins of the innate immune system. Impaired immune function is reported in subjects who are iron deficient, and there are documents that these patients are prone to infection. This study was conducted to show whether serum C3 and C4 complement change in adult nonpregnant female with iron deficient anemia or not. Methods: Forty five normal subjects and 45 iron deficient anemia (hypochrom microcytic) cases were entered in this case and control study by using patients’ clinical history and also results of CBC, Serum ferritin, iron and total iron binding capacity. Serum C3, and C4 were measured in case and control subjects with nephlometry method, finally comparison between result of patients group and control group was done with using suitable statistical test. Results: Mean serum C3 and C4 in patient group was 1.28 ± 0.81 and 0.28 ± 0.23 g/L respectively and for control group was 1.39 ± 0.87 and 0.35 ± 0.25 g/L respectively. Although serum complements were slightly lower in patient groups in compared to control group but this differences was not meaningful with t test. Conclusion: This study showed serum C3 and C4 complements levels were not changed in iron deficiency anemia.
Mohammad Hassan Ghosian Moghaddam; Hossin Ayatollahi; Fatemeh Ghafarirad; Maryam Maleki; Ali Davati
Volume 5, Issue 4 , September 2010, , Pages 173-177
Abstract
Background and Objective: Prostate cancer is a prevalent disease around the world. The prostatic specific Antigen (PSA) test has recently proved itself as a useful method for the diagnosis and examination of patients with prostatic cancer. The objective of this study was to compare the stability of free ...
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Background and Objective: Prostate cancer is a prevalent disease around the world. The prostatic specific Antigen (PSA) test has recently proved itself as a useful method for the diagnosis and examination of patients with prostatic cancer. The objective of this study was to compare the stability of free PSA and total PSA in different storage settings. Materials and Methods: Samples were obtained from 12 men, within the range of 50-70 yr old, who referred to Hazrat-e-Ghaem Hospital, Mashhad, Iran . The sera were separated via centrifuge and stored at room temperature (21-24ºC) for 3, 6, and 9 hours; at 4ºC for 24, 48, and 72 hours; and at -20ºC for 1, 2, and 3 months. Finally, the stability of PSA was compared to that of the control group. The data were analyzed using the statistical software SPSS and paired t-test and repeated measure. Results:In comparison with the average of the control samples, after 3 hours of storage at room temperature, the free PSA concentration had a 30% drop; and after 72 hours of storage in the refrigerator, the average of free PSA had a 34% fall. In addition, the average of the free PSA concentration kept in the freezer for 3 months exhibited an 11% drop. However, the average of total PSA kept in the refrigerator for 72 hours dropped by 6.9%. Finally, over 2 months of storage in the freezer, the average of the total PSA concentration exhibited a decrease of 10.6%. Conclusion: Free PSA, when compared to total PSA in terms of time and storage temperature, shows less stability.
Hossein Ayatollahi; Akbar Safaei; Mohammad Vasei
Volume 5, Issue 3 , June 2010, , Pages 121-126
Abstract
Background and Objectives: Primary amenorrhea is not a disease but a symptom that may result from several quite different causes[NN1] . Common hormonal cause of primary amenorrhea includes constitutional delay, hypothalamic –pituitary dysfunction, chronic systemic disease and absent ...
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Background and Objectives: Primary amenorrhea is not a disease but a symptom that may result from several quite different causes[NN1] . Common hormonal cause of primary amenorrhea includes constitutional delay, hypothalamic –pituitary dysfunction, chronic systemic disease and absent ovarian function. The aim of this study was to estimate the incidence of the chromosomal abnormality referred for karyotyping in patients with primary amenorrhea in southwest of Iran. Material and Methods: Chromosomal analysis was carried out in 220 such cases that were referred from different parts of the south of Iran. The standard protocol for peripheral blood lymphocyte culture was followed for metaphase chromosome preparation and conventional analysis of G-banded chromosome. Results: The frequency of abnormal karyotypes was 20% in primary amenorrhea. The chromosomal abnormalities can be classified into five main types with or without mosaicism. 1-The most frequent karyotype was X chromosome aneuploidies (10%,n=22) 2-Male karyotype 46, XY was present in 5.5 % (n=12). 3-Structural anomalies of the X chromosome were detected in 3.2% (n=7) . 4-Mosaicism of male chromosome constitution and X chromosome aneuploidy was present in two (0.9%) cases (45XO/46XY). 5-Mosaicism of X chromosome aneuploidy and structural anomalies of X chromosome was found in one (0.45%) case [45, X/46X, i (Xq)]. Conclusion: The present study has emphasized that karyotyping is necessary in evaluation of primary amenorrhea. This study also revealed the incidence of chromosomal abnormalities in women with primary amenorrhea in southwest of Iran is similar to that reported in previous literatures. [NN1]File Format: PDF/Adobe Acrobat - Quick View by SК Ten - 1990 - Cited by 5 - Related articles Primary amenorrhea is not a disease but a symptom that may result from several quite different causes. These include such conditions as endocrinological ... smj.sma.org.sg/3104/3104a13.pdf - Similar
Hossein Ayatollahi; Tayyebeh Kianoush; Nema Mohammadian Roshan
Volume 2, Issue 3 , June 2007, , Pages 94-96
Abstract
Background and Objective: Quality control is one of the most important components in order to improve quality assurance in laboratories during analytical steps. For this purpose, coefficient of variation plays an important role. Due to the fast improvement in technology, application of inferential statistics ...
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Background and Objective: Quality control is one of the most important components in order to improve quality assurance in laboratories during analytical steps. For this purpose, coefficient of variation plays an important role. Due to the fast improvement in technology, application of inferential statistics for the comparisons of laboratory techniques, including instrument and chemicals and fast replacement of them would be technically essential. Materials and Methods: In this research study, we tried to determine the precision of one auto-analyzer with refrigerator and the other one without such facility for the analysis of glucose, triglyceride and cholesterol in 30 successive days and compared the coefficient of variations that are an important factor for analytical precision. Results: Comparing the means of aimed groups by paired t-test with P=0.05 in measurement of glucose, triglyceride and cholesterol showed that there was no significant difference between the two instruments. In determination of glucose, triglyceride and cholesterol by auto-analyzer with refrigerator, CV% of these analytes was 1.3%, 1.4%, and 0.9% respectively. By the auto-analyzer without refrigerator, the CV% of those analytes was 1.5%, 2.5% and 1.11% respectively. Conclusion: There is reasonably higher precision for auto-analyzer with refrigerator than the other one.
Hossein Ayatollahi; Maliheh Hasanzade; Mahdi Farzadnia; Mahdi Khabbaz Khoob; Atefeh Rahmanian
Volume 2, Issue 3 , June 2007, , Pages 100-104
Abstract
Background and Objective: The aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-CRP) in preeclampsia and to compare hs-CRP levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. Materials and Methods: Serum hs-CRP levels were ...
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Background and Objective: The aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-CRP) in preeclampsia and to compare hs-CRP levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. Materials and Methods: Serum hs-CRP levels were investigated in 40 cases of normal pregnant women, 37 cases with mild preeclampsia and 38 cases with severe preeclampsia in the third trimester of pregnancy. Venous blood samples were collected at admission to the hospital at least 6h before delivery for measurement of hs-CRP by immuno turbidometric method. The student t-test was used for comparison of proportions. Results: There were significant difference in the means serum hs-CRP between normal pregnant women and mild preeclamptic women (P<0.05). Serum concentration of hs-CRP were significantly higher in severe prceclampsia (p<0.05) than normal pregnancy. There were also significant differences in hs-CRP levels between mild and severe preclampsia (P<0.05). Conclusion: We found higher levels of hs-CRP in mild and severe preeclampsia than normal pregnancy and also these results suggest that hs- CRP are increased more in severe preeclamsia than mild preeclampsia , and may be useful in prediction and diagnosis of the severity of preeclampsia.
Hossein Ayatollahi; Tayebeh Kianoush; Mohammad Khajeh Daluee
Volume 1, Issue 2 , April 2006, , Pages 65-68
Abstract
Background and Objectives: For total analytical error, imprecision (SD) and bias, performance goals for laboratory tests have most often been developed. A total analytical error goal requires that the combination of errors from all sources (random and systematic errors) be within some acceptable limit. ...
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Background and Objectives: For total analytical error, imprecision (SD) and bias, performance goals for laboratory tests have most often been developed. A total analytical error goal requires that the combination of errors from all sources (random and systematic errors) be within some acceptable limit. Materials and Methods: Fifty vials of sodium and potassium standards (Sandos Company) were chosen. Then, the concentration of sodium and potassium in these standards were daily measured using flame photometry for 50 days at biochemistry laboratory (Ghaem hospital, Mashhad). Thereafter, the mean, standard deviation, random and systematic analytical errors, and total analytical error from these values were calculated. Results: The systematic and random analytical errors for standard specimens using flame photometry method for sodium and potassium are με (Na) = 0.36, με (k) = 0.012, σε (Na) = 0.69 and σε (k) = 0.11 respectively. Meanwhile, the total analytical error of flame photometry for measurement of sodium and potassium was 1.74 mM/l and 0.232 mM/l respectively. Conclusion: In this study, it was found out that flame photometry system has good precision and accuracy and its total analytical error for measuring of sodium and potassium are within the acceptable range.