Original Research
Sethi Bhawna; Arora Bharti; Kumar Yogesh; Aggarwal Reena
Volume 8, Issue 1 , January 2013, Pages 1-8
Abstract
Background& Objectives: Inspite of intensive worldwide efforts to reduce its transmission, malaria remains the most serious and widespread protozoal infection of humans. It is a protozoan disease transmitted by the bite of infected female anopheles mosquito. Malaria has long featured prominently ...
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Background& Objectives: Inspite of intensive worldwide efforts to reduce its transmission, malaria remains the most serious and widespread protozoal infection of humans. It is a protozoan disease transmitted by the bite of infected female anopheles mosquito. Malaria has long featured prominently in the grey area between parasitology and hematology. This study has been performed to evaluate various hematological alterations in patients infected with malaria and to add more detailed information, especially from these highly affected zones.
Materials and methods: A two-year, hospital-based study was conducted and hematologic profiles of 200 persons infected with malaria (Plasmodium vivax or Plasmodium falciparum) infection, were examined and the results were compared with standard normal values.
Results: One-hundred-ninety-six patients were infected with Plasmodium vivax, three with Plasmodium falciparum, and one was infected with both. Patients with parasitemia tended to have significantly lower platelets, hemoglobin, white blood cell and red blood cell counts, blood indices and hematocrit than normal. Thrombocytopenia is identified as a key indicator of malaria in these febrile patients.
Conclusion: Anemia and thrombocytopenia are the classical changes. Changes in the white blood cells are less dramatic, may vary due to variable size and type of cases, variability of the species, and geographical differences.
Original Research
Hamid Agha-Alinejad; Mohammad-Ali Kohanpour; Suzan Sanavi; Soghra Sojudi; Gholam-Reza Behrouzi; Mona Mirsepasi
Volume 8, Issue 1 , January 2013, Pages 9-16
Abstract
Background & Objectives:Endocrine hormones, through their anabolic or catabolic function, contribute in body homeostasis. They can be used to assess the physical performance in athletes. This study was performed to determine the resistance training (RT) influences on serum cortisol and dehydroepiandrosterone ...
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Background & Objectives:Endocrine hormones, through their anabolic or catabolic function, contribute in body homeostasis. They can be used to assess the physical performance in athletes. This study was performed to determine the resistance training (RT) influences on serum cortisol and dehydroepiandrosterone in trained young females.
Materials & Methods:The study population consisted of 36 women (20-25 years) who were divided into 2 experimental groups (A, B) and control group (C). Experimental groups were scheduled to do 8-week incremental RT program, intermittently (A) or continuously (B), through alterations in the prescribed training velocity. Two days before starting and after ending of program, in a testing session of RT with the intensity of 20% one repetition maximum strength, blood samples for measuring cortisol and dehydroepiandrosterone were obtained (a pre-post test design). The SPSS version 16 was used for statistical analyses.
Results: Serum cortisol of experimental groups showed significant decline (P=0.002 in each group) without significant difference between two groups (P =0.819), while, DHEA and DHEA/Cortisol ratio had significant increase (all P =0.000) with no significant difference between two groups (P DHEA=0.275 and P ratio= 0.998) at both stages.
Conclusion: With considering the role of RT in establishing an anabolic status following exercise, it seems that young women can use different types of RT to improve their physical performance. This conclusion needs more researches regarding RT
Original Research
Majid Sharbatdaran; Sepideh Siadati; Mahtab Zeinalzadeh; Shahriar Shafaei; Zahra Basirat; Amir Esmi
Volume 8, Issue 1 , January 2013, Pages 17-20
Abstract
Background: Cervical cancer is the second most common cancer in the world among women and human papilloma virus (HPV) plays a major role in its development. The aim of this study was to determine the frequency of HPV type 16 and 18 in cervical discharge by polymerase chain reaction (PCR) method ...
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Background: Cervical cancer is the second most common cancer in the world among women and human papilloma virus (HPV) plays a major role in its development. The aim of this study was to determine the frequency of HPV type 16 and 18 in cervical discharge by polymerase chain reaction (PCR) method in women with atypical biopsy or papsmear.
Method: This case- control study was performed on women in Yahyanejad Hospital, Babol University of Medical Sciences, Babol, Iran during 2008-2009. Sixty women with normal papsmear (group1) and 30 women with atypical papsmear or biopsy (group 2) were enrolled in the study and their cervical discharge was assessed for HPV type 16 and 18. Data was analyzed with SPSS, Chi-Square, Fisher,s Exact test and t-test and P<0.05 was considered significant.
Results: HPV type 16 was founded in 10% women of group 2 but not seen in group1. HPV 18 was not detected. All women had one partner and none of them had alcohol consumption.
Conclusion: In comparison with other studies, the frequency of HPV infection is lower in our study. We considered this is strongly related to our culture and religious beliefs.
Original Research
Maryam Amini; Ali Davati; Masomeh Piri
Volume 8, Issue 1 , January 2013, Pages 21-26
Abstract
Background and Objective: Diabetes mellitus is one of the main problems in health systems in the world. Diabetic Foot infections (DFI) is one of the main complications and the most cause of non-traumatic lower limb amputation .This study aimed to determine the prevalence of bacteria involved in ...
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Background and Objective: Diabetes mellitus is one of the main problems in health systems in the world. Diabetic Foot infections (DFI) is one of the main complications and the most cause of non-traumatic lower limb amputation .This study aimed to determine the prevalence of bacteria involved in DFI and their antibiotic resistance in patients with DFI diagnosis.
Patients and Methods: This descriptive-analytical and cross-sectional study was designed in 4 years period from April 2007 to March 2010 on 90 patient in Shahid Mostafa Khomeini Hospital,Tehran, Iran .For bacteriological analysis, all wound samples culture grown by standard methods bacteriology and Disk diffusion method was used for antibiogram. Patient’s clinical and epidemiologic data were collected from recorded file. The data were analyzed by using SPSS16 statistical software.
Results: 104 bacteria were isolated from 90 patients. 57.70% were gram-positive and 42.30% were gram-negative. Among gram-positive bacteria Staphylococcus aureus (60%) and enteroccoci spp.(33.3%) and among gram-negative bacteria E. coli (47.73%), Pseudomonas Aeroginosa (22.73%) and Proteus spp.(18.18%) were the most common isolates respectively. 75% of isolates were resistant to two antibiotics or more. Previous antibiotic therapy was significant risk factor for multidrug resistant (MDR) infections (P: 0.003). All gram-positive isolates were sensitive to Vancomycin. Imipenem and Amikacin had good activity against gram-negative bacteria.
Conclusion:Infection with MDR bacteria in patients with diabetic foot ulcers is high and have significant association with recent antibiotic therapy. So the proper use of antibiotics in order to prevent the creation of multi-drug resistant bacteria is recommended.
Original Research
Ali Zare Mehrjardi; Amineh Vaghefi
Volume 8, Issue 1 , January 2013, Pages 27-35
Abstract
Background & Objectives: Anaplastic lymphoma Kinase (ALK) is a receptor tyrosine kinase involved in the genesis of several human cancers. ALK was initially identified because of its involvement in anaplastic large cell lymphoma (ALCL). ALK is believed to foster tumorigenesis following activation ...
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Background & Objectives: Anaplastic lymphoma Kinase (ALK) is a receptor tyrosine kinase involved in the genesis of several human cancers. ALK was initially identified because of its involvement in anaplastic large cell lymphoma (ALCL). ALK is believed to foster tumorigenesis following activation by autocrine and/or paracrine growth loops. Studies reveal that the presence of anti-ALK antibodies may be relevant to the relatively good prognosis. Therapeutic approaches consisting of gene therapy and immunotherapy targeting this molecule hold promise.
Material & Methods: We examined a number of human breast cancers to see if ALK is expressed in this tumor and studied its relation with type of carcinoma and its grade, tumor size, presence of necrosis, vascular invasion , skin involvement, lymph node metastasis and patient’s age.
Result: 100 patients were enrolled with mean age of 50.2 ± 12.5 years. The histological phenotypes of the breast cancers studied included Invasive Ductal Carcinoma, Invasive Lobular Carcinoma and Medullary Carcinoma. ALK expression was evaluated by immunohistochemistry which was positive in 47 cases (47%). No statistically significant relationship is found between the above mentioned parameters except for tumor size and ALK expression. (P< 0.01)
Original Research
Fatemeh Fattahi; Alireza Mirvaghefi; Hamid Farahmand; Gholamreza Rafiee; Alireza Abdollahi
Volume 8, Issue 1 , January 2013, Pages 36-44
Abstract
Objectives: The presence of E.coli in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. The objective of this study was to develop ...
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Objectives: The presence of E.coli in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. The objective of this study was to develop an improved PCR method based on species – specific 16 S rRNA gene primers (FES, RES) for detection of E. coli from agar plates and fish tissues.
Materials and Methods: In this study, For the rapid detection of E .coli from fish a set of primers (FES, RES), targeting 16S rRNA gene sequences of the specific microorganism was designed, and fifty two rainbow trout were obtained from Karaj fish farm. Then 1mL of bacterial concentration of 106CFU/ml was injected into intraperitoneal cavity. Samples were collected from liver and kidney after 48h injection. The PCR reaction conditions were optimized to permit detection of organism from agar plates and fish tissue in a day.
Results: All tissue samples were positive for microbiological and PCR identification. DNA was successfully extracted by a boiled – extraction method or by phenol – chloroform – isoamyl alcohol. The BLAST analysis from sequencing of 4 amplicons randomly selected showed similar results, with the match being E .coli with a 100% similarity (not shown here).
Conclusion: It is concluded that this method is fast, specific and sensitive to detect E.coli in infected and asymptomatic animals, fish product, and may have a positive impact on public and environmental health.
Case Reports
Raphael Hart Lyngdoh; Sumanth Devaraju; Leena JB
Volume 8, Issue 1 , January 2013, Pages 45-47
Abstract
Hamartomatous polyps of the palatine tonsil are very rare. They have been variously termed as a lymphangiectatic fibrous polyp, lipoma, pedunculated tonsil etc. in the English literature. We present here a case of hamartomatous polyp of the tonsil occurring in a 32-year-old male who presented ...
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Hamartomatous polyps of the palatine tonsil are very rare. They have been variously termed as a lymphangiectatic fibrous polyp, lipoma, pedunculated tonsil etc. in the English literature. We present here a case of hamartomatous polyp of the tonsil occurring in a 32-year-old male who presented with bilateral enlarged palatine tonsils with recurrent tonsillitis. Surgical excision showed two pale brown nodular tissue bits, larger measuring 4x3x1 cm and smaller measuring 3.5x2.5x1.5 cm. An irregular pedunculated polypoidal tissue measuring 1.5x1x0.8cm was seen attached to the smaller mass, the cut surface of which showed pale white, lobulated areas. On histological examination, the polyp showed lining by stratified squamous epithelium and was composed of fibrocollagenous stroma with many dilated blood vessels, adipocytes, subepithelial lymphoid aggregates and benign mucinous glands suggestive of a hamartomatous polyp. Both the tonsils showed features of chronic tonsillitis.
Case Reports
Zhaleh Mohsenifar1; Nazanin Mahdavi; Sara Bagheri
Volume 8, Issue 1 , January 2013, Pages 48-54
Abstract
Lymphoepithelial cyst of oral cavity is a rare cystic lesion that presents as an asymptomatic submucosal mass and is usually discovered during routine dental examinations. The site most commonly affected is floor of the mouth and tonsillar involvement seems to be very rare. Multiple Lymphoepithelial ...
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Lymphoepithelial cyst of oral cavity is a rare cystic lesion that presents as an asymptomatic submucosal mass and is usually discovered during routine dental examinations. The site most commonly affected is floor of the mouth and tonsillar involvement seems to be very rare. Multiple Lymphoepithelial cysts have been reported in parotid of HIV positive patients however in oral cavity they usually present as solitary lesions.We report a case of multiple bilateral Lymphoepithelial cysts of the palatine tonsils in a 72 years old male that presents with fever, pain and obstructive sleep apnea.We didn’t find any bilateral or multiple tonsillar lymphoepiyhelial cysts in the literature and the present paper seems to be the first report. We suggest although lymphoepithelial cysts rarely occur in the tonsils but they should be considered in differential diagnosis of adenotonsillar enlargement and related obstructive sleep apnea.
Case Reports
Maryam Abolhasani; Mojgan Asgari; Hossein Keymoosi
Volume 8, Issue 1 , January 2013, Pages 55-58
Abstract
Splenogonadal fusion is a rare entity with approximately 150 reported cases until the year 2005. The entity is a rare congenital anomaly in which there is fusion of splenic and gonadal anlagen or mesonephric derivatives. Splenogonadal fusion has two continuous and discontinuous types. About ten cases ...
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Splenogonadal fusion is a rare entity with approximately 150 reported cases until the year 2005. The entity is a rare congenital anomaly in which there is fusion of splenic and gonadal anlagen or mesonephric derivatives. Splenogonadal fusion has two continuous and discontinuous types. About ten cases have been reported to be associated with bilateral cryptorchidism in literature. Very few cases have been diagnosed preoperatively. Many cases present as a testicular swelling and undergo an unnecessary orchiectomy with the suspicion of a testicular neoplasm. Herein, we report a new case of discontinuous splenogonadal fusion in a 29 years old man with bilateral cryptorchidism who underwent surgery. Surprisingly two masses were noted adjacent to undescendent testis in left groin in operative room which were resected and proved to be a new case of splenogonadal fusion in histological exam.
Case Reports
Anuradha Sinha; Jyoti Prakash Phukan; Gautam Bandyopadhyay; Sanjay Sengupta; Rinki Saha
Volume 8, Issue 1 , January 2013, Pages 59-63
Abstract
Superficial acral fibromyxoma are rare soft tissue tumors predominantly involving hands and feet of adults. There are only a few reported cases of this benign neoplasm, but no FNAC diagnosis of this tumor is reported in the literature. A Forty-eight year old female presented with a 5 x 3.5 cm globular, ...
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Superficial acral fibromyxoma are rare soft tissue tumors predominantly involving hands and feet of adults. There are only a few reported cases of this benign neoplasm, but no FNAC diagnosis of this tumor is reported in the literature. A Forty-eight year old female presented with a 5 x 3.5 cm globular, firm swelling over the right fifth toe. Fine needle aspiration cytology (FNAC) of the lesion was interpretated as superficial acral fibromyxoma, and Subsequent histopathology confirmed the diagnosis and demonstrated cytohistological correlation for the first reported time in the literature. SAFM may recur if not adequately excised. Preoperative FNAC diagnosis will help the surgeons to plan for wider excision to prevent recurrence.
Case Reports
Pinki Pandey; Alok Dixit; Aparna Tanwar; Nanak Chand Mahajan
Volume 8, Issue 1 , January 2013, Pages 64-70
Abstract
Endometrial stromal sarcoma represents a very rare pathological entity occurring as a malignant disease in women genital sphere, frequently in the age group of 40-50 years. Low grade endometrial stromal sarcoma (LGESS) is a rare uterine sarcoma constituting 0.2% of all the uterine malignancies with a ...
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Endometrial stromal sarcoma represents a very rare pathological entity occurring as a malignant disease in women genital sphere, frequently in the age group of 40-50 years. Low grade endometrial stromal sarcoma (LGESS) is a rare uterine sarcoma constituting 0.2% of all the uterine malignancies with a good prognosis despite a tendency to recur. We report a case of 42 years old woman presented with menorrhagia, dysmenorrhoea, anemia and rapidly enlarging mass in uterus clinically diagnosed as leiomyoma, underwent a hysterectomy. Histological examination revealed cells with ovoid to short, spindle shaped nuclei resembling endometrial stromal cells proliferating in a space occupying manner and compressing and partially infiltrating the myometrium, rendered the diagnosis of LGESS. Our case emphasizes the inevitability for high index of skepticism and proper preoperative diagnosis in this rare tumor.
Letters to the Editor
Kamon Chaiyasit; Viroj Wiwanitki
Volume 8, Issue 1 , January 2013, Pages 71-71