Document Type : Original Research
Authors
1 Dept. of Physiology, Islamic Azad University, Qom, Iran
2 Razi Clinical Lab., Ahwaz, Iran
3 Dept. of Microbiology, Jondishapour University of Medical Sciences, Ahwaz, Iran
Abstract
Background and Objectives: Type 2 diabetes is the most prevalent form of diabetes mellitus and is associated with a variety of complications. Homocysteine is an important independent risk factor for atherosclerotic diseases in both diabetic and non diabetic subjects. The association between these two is still unclear. The aim of this study was to assess the serum homocysteine levels in uncomplicated type 2 diabetic patients and control subjects.
Materials and Methods: Eighty five diabetic patients and 85 healthy control subjects with the mean age of 57.65 and 57.68 years, respectively, were selected during 2010 in Ahwaz City, southwest of Iran. Serum glucose, lipids and lipoproteins were measured by standard enzymatic techniques and homocysteine levels by enzyme –linked immunosorbent assay method.
Results: In patients as a whole and with respect to the gender, homocysteine levels were generally lower than controls. Compared to other studies, homocysteine levels ranging from 12.19 to 18 µmol/l were slightly higher in both population.
Conclusion: Homocysteine levels, however, were compatible with normal range of adults. The patients were not nephropathic and it is most likely that this is the main reason for maintaining the normal levels. Slightly higher levels of homocysteine in the region are due to multiple genetic and environmental factors.
Keywords
- Zimmet P, Alberti KG, Shaw J. Global and societal implications of the diabetes epidemic. Nature 2001 Dec 13;414(6865):782-7.
- Yamada T, Sato A, Nishimori T, Mitsuhashi T, Terao A, Sagai H, et al. Importance of hypercoagulability over hyperglycemia for vascular complication in type 2 diabetes. Diabetes Res Clin Pract 2000 Jul;49(1):23-31.
- Carr ME. Diabetes mellitus: a hypercoagulable state. J Diabetes Complications 2001 Jan;15(1):44-54.
- Glueck CJ, Shaw P, Lang JE, Tracy T, Sieve-Smith L, Wang Y. Evidence that homocysteine is an independent risk factor for atherosclerosis in hyperlipidemic patients. Am J Cardiol 1995 Jan 15;75(2):132-6.
- Perry IJ, Refsum H, Morris RW, Ebrahim SB, Ueland PM, Shaper AG. Prospective study of serum total homocysteine concentration and risk of stroke in middle-aged British men. Lancet 1995 Nov 25;346(8987):1395-8.
- Masuda Y, Kubo A, Kokaze A, Yoshida M, Fukuhara N, Takashima Y. Factors associated with serum total homocysteine level in type 2 diabetes. Environ Health Prev Med 2008 May;13(3):148-55.
- Mazza A, Bossone E, Mazza F, Distante A. Reduced serum homocysteine levels in type 2 diabetes. Nutr Metab Cardiovasc Dis 2005 Apr;15(2):118-24.
- Sen U, Tyagi SC. Homocysteine and Hypertension in Diabetes: Does PPARgamma Have a Regulatory Role? PPAR Res 2010;2010:806538. Epub;%2010 Jun 29.:806538.
- Rudy A, Kowalska I, Straczkowski M, Kinalska I. Homocysteine concentrations and vascular complications in patients with type 2 diabetes. Diabetes Metab 2005 Apr;31(2):112-7.
- Definition , diagnosis and classification of diabetes mellitus and it's complications. 1999. Geneva, World Health Organization.
Ref Type: Serial (Book,Monograph) - Burtis C, Ashwood E. Tietz textbook of Clinical Chemistry. 3 ed. Philadelphia: WB Saunders; 1999.
- Narang AP, Verma I, Kaur S, Narang A, Gupta S, Avasthi G. Homocysteine--risk factor for ischemic stroke? Indian J Physiol Pharmacol 2009 Jan;53(1):34-8.
- El OM, Aouni Z, Ouertani H, Mazigh C, Machghoul S. Effect of lipopenic and hypotensive treatment on homocysteine levels in type 2 diabetics. Vasc Health Risk Manag 2010 May 25;6:327-32.:327-32.
- Soares AL, Fernandes AP, Cardoso JE, Sousa MO, Lasmar MC, Novelli BA, et al. Plasma total homocysteine levels and methylenetetrahydrofolate reductase gene polymorphism in patients with type 2 diabetes mellitus. Pathophysiol Haemost Thromb 2008;36(5):275-81.
- Faulkner MS, Chao WH, Kamath SK, Quinn L, Fritschi C, Maggiore JA, et al. Total homocysteine, diet, and lipid profiles in type 1 and type 2 diabetic and nondiabetic adolescents. J Cardiovasc Nurs 2006 Jan;21(1):47-55.
- Eikelboom JW, Lonn E, Genest J, Jr., Hankey G, Yusuf S. Homocyst(e)ine and cardiovascular disease: a critical review of the epidemiologic evidence. Ann Intern Med 1999 Sep 7;131(5):363-75.
- Frantzen F, Faaren AL, Alfheim I, Nordhei AK. Enzyme conversion immunoassay for determining total homocysteine in plasma or serum. Clin Chem 1998 Feb;44(2):311-6.
- Mazza A, Giugliano D, Motti C, Cortese C, Andreotti F, Marra G, et al. Glycemia, MTHFR genotype and low homocysteine in uncomplicated type 2 diabetic patients. Atherosclerosis 2000 Mar;149(1):223-4.
- Cho EH, Kim EH, Kim WG, Jeong EH, Koh EH, Lee WJ, et al. Homocysteine as a risk factor for development of microalbuminuria in type 2 diabetes. Korean Diabetes J 2010 Jun;34(3):200-6.
- Schneede J, Refsum H, Ueland PM. Biological and environmental determinants of plasma homocysteine. Semin Thromb Hemost 2000;26(3):263-79.
- Refsum H, Smith AD, Ueland PM, Nexo E, Clarke R, McPartlin J, et al. Facts and recommendations about total homocysteine determinations: an expert opinion. Clin Chem 2004 Jan;50(1):3-32.
- Bazzano LA. Folic acid supplementation and cardiovascular disease: the state of the art. Am J Med Sci 2009 Jul;338(1):48-9.
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