Document Type : Original Research
Dept. of Clinical Research, Pasteur Institute of Iran, Tehran, Iran
TPIRC (Tuberculosis and Pediatric Infectious Research Center), Arak University of Medical Sciences, Arak, Iran
Iranian Society for Support of Patients with Infectious Disease, Tehran, Iran
Blood Transfusion Organization Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Background and Objectives: Laboratory reference values are an important tool for clinical management of patients. Reference values being used in most laboratories in Iran have been provided from the established reference values from developed countries. However, several variables can affect on these laboratory parameters. Therefore, this study was carried out to establish the reference values of hematological parameters in the blood donors of central province of Iran as a general population.
Methods: Blood samples of 1100 male blood donors were collected consecutively from Blood Transfusion Organization. Complete blood cell (CBC) count in 2012 and differential was performed using an automated hematology analyzer.
Results: The median and 95% reference values (2.5th-97.5th) for Hb and platelet counts were 15.5 g/dl (14.1-17.7) and 209 ×109 cells/L (151-322) respectively. The median for total WBC count, neutrophil, lymphocyte, monocyte and eosinophil were 6.7 ×109 cells/L (4.3-11.2), %58 (%50-%70), 40% (30-49%), 0% (0-2) and %1 (0-3%), respectively.
Conclusion: The hematological profile of the population in central province of Iran was different from the reports of other countries and also the standards reference ranges described in textbook. So, further nationwide study should be carried out to establish the hematological reference values of the Iranian population as a whole.
- Haileamlak A, Muluneh AT, Alemseged F, Tessema F, Woldemichael K, Asefa M, et al. Hematoimmunological profile at gilgel gibe field research center, southwest Ethiopia. Ethiop J Health Sci 2012; 22(S):39-50
- Buchanan AM, Muro FJ, Gratz J, Crump JA, Musyoka AM, Sichangi MW, et al. Establishment of haematological and immunological reference values for healthy Tanzanian children in Kilimanjaro Region. Trop Med Int Health 2010; 15:1011–21
- Horn PS, Pesce AJ. Effect of ethnicity on reference intervals. Clin Chem 2002; 48:1802–1804.
- El-Hazmi MAF, Warsy AS. Normal reference values for the hematological parameters, red cell indices, HbA2 and Hb F from early childhood through adolescence in Saudis. Ann Saudi Med 2001;2:165–9
- Usman K, Syed ZA, Rao AA. Reference Range Values of Hematological Parameters in Healthy Pakistani Adults. Pak J Physiol 2007; 3(1):19-22.
- Solberg HE. International Federation of Clinical Chemistry (IFCC), Scientific Committee, Clinical Section, Expert Panel on Theory of Reference Values, and International Committee for Standardization in Hematology (ICSH), Standing Committee on Reference Values. Approved Recommendation on the theory of reference values. Part 1. The concept of reference values. J Clin Chem Clin Biochem 1987;25:337–42
- CLSI. Defining, establishing, and verifying reference intervals in the clinical laboratory: approved guideline. Wayne, PA: Clinical and Laboratory Standards Institute; 2008.
- Dosoo DK, Kayan K, Adu-Gyasi D, Kwara E, Ocran J, Osei-Kwakye K, et al. Haematological and Biochemical Reference Values for Healthy Adults in the Middle Belt of Ghana. PLoS One 2012; 7(4): e36308.
- Koram K, Addae M, Ocran J, Adu-Amankwah S, Rogers W, Nkrumah F. Population based reference intervals for common blood haematological and biochemical parameters in the Akuapem north district. Ghana Med J 2007; 41(4):160-6.
- Roshan TM, Rosline H, Ahmed SA, Rapiaah M, Wan Zaidah A, Khattak MN. Hematological reference values of healthy Malaysian population. Int J Lab Hematol 2009: 31: 505-512.
- Lugada ES, Mermin J, Kaharuza F, Ulvestad E, Were W, Langeland N, et al. Population-Based Hematologic and Immunologic Reference Values for a Healthy Ugandan Population. Clin Diagn Lab Immunol 2004:11(1)29-34.
- Kibaya RS, Bautista CT, Sawe FK, Shaffer DN, Sateren WB, Scott PT, et al. Reference Ranges for the Clinical Laboratory Derived from a Rural Population in Kericho, Kenya. PLoS One 2008; 3 (e 10):e3327.
- Saathoff E , Schneider P, Kleinfeldt V, Geis S, Haule D, Maboko L, et al. Laboratory reference values for healthy adults from southern Tanzania. Trop Med Int Health 2008; 13:612-625.
- Karita E, Ketter N, Price MA, Kayitenkore K, Kaleebu P, Nanvubya A, et al. CLSI-Derived Hematology and Biochemistry Reference Intervals for Healthy Adults in Eastern and Southern Africa. PloS one 2009; 4(2): e4401.
- Kasper DL, Fauci AS, Longo DL, (Eds). In: Harrisons Principles of Internal Medicine. 18th ed. McGraw-Hill Companies, Inc. 2012.
- Kaya H, Kyky Y, Akarsu E, Gündoddu M, BaÞol Tekyen SB, Ýnandi T. Hematological Values of Healthy Adult Population Living at Moderate Altitude (1869 m, Erzurum, Turkey). Turk J Haematol 2000; 17(3):123-8.
- Kratz A, Ferraro M, Sluss PM, Lewandrowski KB. Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Laboratory reference values. N Engl J Med 2004; 351:1548–63.
- Bain BJ. Ethnic and sex differences in the total and differential white cell count and platelet count. J Clin Pathol 1996; 49:664-6.
- Shaper AG, Lewis P. Genetic neutropenia in people of African origin. Lancet 1971; 2(7732):1021-3.
- Badenhorst CJ, Fourie J, Steyn K, Jooste PL, Lombard CJ, Bourne L, et al. The haematological profile of urban black Africans aged 15-64 years in the Cape Peninsula. East Afr Med J 1995; 72:19-24.