Document Type: Original Research


1 Dept. of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Background & Objectives:  CD10 is a cell surface enzyme with metalloendopeptidase activity, also known as Common Acute Lymphoblastic Leukaemia Antigen, which mainly serves as a marker for acute lymphoblastic leukemia (ALL). To date and to the best of our knowledge, only few comparative immunohistochemical studies have assessed CD10 expression in cutaneous epithelial neoplasms. Our goal was to determine whether CD10 can be used in pathologic distinction of cutaneous basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Methods: This study included 27 BCC and 17 SCC cases   immunohistochemically stained for CD10. Cytoplasmic +/- cell membrane staining was considered as positive. Each slide was studied by two pathologists and scored semi- quantitatively as follows: negative (<10%); 1+ (10-50% positive cells); and 2+ (>50% positive cells). Results: The rate of CD10 expression in tumor cells was significantly higher in BCCs in comparison to SCCs. (20/27 vs., 2/17; P <0.0001). Discussion: Our findings suggest CD10 as a useful adjunct marker in distinguishing cutaneous BCC and SCC.


1. McIntosh GG, Lodge AJ, Watson P, Hall AG, Wood K, Anderson JJ, Angus B, Horne CH, Milton ID. NCL-CD10-270: A new monoclonal antibody recognizing CD10 in paraffin embedded tissue. Am J Pathol 1999; 154(1):77-82.

2. Moritani S, Kushima R, Sugihara H, Bamba M, Kobayashi TK, Hattori T.. Availability of  CD10  Immunohistochemistry as a marker of breast myoepithelial cells on paraffin sections. Mod Pathol 2002;15(4):397-405.

3. Gafford JT, Skidgel RA, Erdös EG, Hersh LB., Human kidney “enkephalinase”, a neutral metalloendopeptidase that cleaves active peptides. Biochemistry 1983;22(13):3265-71.

4. Takahara M, Chen S, Kido M, Takeuchi S, Uchi H, Tu Y, et al. Stromal CD10 expression, as well as increased dermal macrophages and decreased Langerhans cells, are associated with malignant transformation of keratinocytes. J Cutan Pathol 2009;36:668-74.

5. Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S. Expression of CD10  in basal cell carcinoma. Am J Dermatopathol 2004; 26(6):463-71.

6. Sengul D, Sengul I, Astarci MH, Ustun H,Mocan G. CD10 for the distinct differential diagnosis of basal cell carcinoma and benign tumours of cutaneous appendages originating from hair follicle. Pol J Pathol 2010;61:140-6.

7. Wagoner J, Keehn C, Morgan MB. CD-10 Immunostaining Differentiates Superficial Basal Cell Carcinoma from Cutaneous Squamous Cell Carcinoma. Am J Dermatopathol 2007; 29 (6):555-8.

8. Sanders DSA, Carr RA. The use of immunohistochemistry in the differential diagnosis of common epithelial tumours of the skin. Current Diag Pathol 2007; 13(4):237–51.

9. Oba J, Nakahara T, Hayashida S, Kido M, Xie L, Takahara M, et al. Expression of CD10 predicts tumor progression and unfavorable prognosis in malignant melanoma. J Am Acad Dermatol 2011;  65:1152-60.

10. Aiad HA, Hanout HM. Immunohistochemical Expression of CD10 in cutaneous Basal and Squamous Cell Carcinomas. J Egypt Nat Cancer Inst 2007;19(3):195-201.

11. Pham TT, Selim MA, Burchette JL Jr, Madden J, Turner J, Herman C. CD10 expression in tichoepithelioma and basal cell carcinoma. J  Cutan Pathol 2006;33(2):123-8.

12.Carr RA, Sanders D.S.A. Basaloid skin tumours: Mimics of basal cell carcinoma. Current Diag Pathol 2007; 13(4):273–300.

13. Swanson PE, Fitzpatrick MM, Ritter JH, Glusac EJ, Wick MR.Immunohistologic differential diagnosis of basal cell carcinoma, squamous cell carcinoma, and trichoepithelioma in small cutaneous biopsy specimens. J  Cutan Pathol 1998; 25:153–9.

14. Verhaegh ME, Arends JW, Majoie IM, Hoekzema R, Neumann HA. Transforming growth factor-beta and bcl-2 distribution patterns distinguishing trichoepithelioma from basal cell carcinoma. Dermatol Surg 1997;23(8):695–700.

15. Cassarino DS, Derienzo DP, Barr RJ. Cutaneous squamous cell carcinoma: a comprehensive clinicopathologic classification. Part one. J Cutan Pathol 2006;33:191–206.

16. Sakiz D, Turkmenoglu TT, Kabukcuoghlu F. The expression of p63 and p53 in keratoacanthoma and intraepidermal and invasive neoplasms of the skin. Pathol Res Prac 2009; 205(9):589-94.