The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis

Document Type: Original Research


1 Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

2 Dept. of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

3 Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

4 Dept. of Laboratory Sciences, Para-Medical School, Kerman University of Medical Sciences, Kerman, Iran

5 Dept. of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran

6 Dept. of Histology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

7 Dept. of Immunology, Medical School, Tarbiat Moddares University, Tehran, Iran

8 Dept. of Immunology, Medical School, Tarbiat Modares University, Tehran, Iran



Background & objective The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE).
Methods: EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively.
Results: IL-12 gene expression and its serum levels in PBS-injected- or OO-administrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected- or OO-administrated EAE groups (P Conclusion: VD modulates the expression of IL-12 and TGF-β in spinal cord and serum of EAE mice.


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