Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard

Askari, Nayere; Ghazanfari, Tooba; Naghizadeh, Mohammad Mehdi; Moin, Athar; Khamesipour, Ali; Pourfarzam, Shahryar; Hassan, Zuhair Mohammad

doi:10.30699/ijp.2017.25646

Document Type : Original Research

Authors

¹ Dept. of Biology, Faculty of Basic Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

² Immunoregulation Research Center, Shahed University, Tehran, Iran

³ Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran

⁴ Dept. of Immunology, Tarbiat Moddares University, Tehran, Iran

10.30699/ijp.2017.25646

Abstract

Background & objective: Pruritus is the most frequent chronic dermal complication of sulfur mustard (SM), which negatively influences the quality of life. Exact pathophysiology of SM-induced itching is unknown. The current study aimed at evaluating the possible association between SM-induced itching and the serum levels of matrix metalloproteinase (MMP)-9 and their endogenous inhibitors, and serum levels of soluble forms of selectins (sL-, sP-, and sE-selectins) as adhesion molecules involved in the development of different inflammatory reactions.
Methods: Serum levels of MMP-9, MMP-9/ tissue inhibitors of metalloproteinases (TIMPs), and selectins were measured by the enzyme-linked immunosorbent assay (ELISA), and compared between the groups (n=368) with and without itching, and matched control groups (n=126).
Results: Serum levels of MMP-9 were significantly higher in the SM exposed group with itching, compared with that of the group without itching (medians: 894 and 624 pg/mL respectively; P-value =0.034). There was no relationship between the serum levels of MMP-9/TIMP-1, MMP-9/TIMP-2, MMP-9/TIMP-4, and itching in the patients exposed to SM. Median serum levels of sE- and sL-selectins in the exposed group with itching were higher than those of the exposed group without itching. These differences were statistically insigniﬁcant (P-values =0.084 and 0.095, respectively).
Conclusion: According to the results of the current study, the increased serum levels of MMP-9 and selectins 20 years after exposure may play role in the pathogenesis and persistence of SM-induced itching in the exposed individuals.

Keywords

Main Subjects

Dermatopathology

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Iranian Journal of Pathology

Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard

References

References

Volume 12, Issue 3
Summer 2017
Pages 257-264

Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard

References

References

Volume 12, Issue 3Summer 2017Pages 257-264

Volume 12, Issue 3
Summer 2017
Pages 257-264