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Rostami, S., Pasdar, A., Gerayli, S., Hatami, H., Sepahi, S., Nategh, F., Meshkat, M., Hoseini, S., Ahadi, M., Sima, H., Vosughinia, H., Sarvghad, M., Esmaeelzade, A., Nomani, H., Mosanan Mozafari, H., Rezai Talab, F., Shakeri, M., Meshkat, Z. (2017). Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran. Iranian Journal of Pathology, 12(3), 248-256.
Sina Rostami; Alireza Pasdar; Sina Gerayli; Hamed Hatami; Samaneh Sepahi; Fatemeh Nategh; Mojtaba Meshkat; Seyed Mousalreza Hoseini; Mitra Ahadi; Hamid Reza Sima; Hasan Vosughinia; Mohammad Reza Sarvghad; Abbas Esmaeelzade; Hosein Nomani; Homan Mosanan Mozafari; Fariba Rezai Talab; Mohammad Taghi Shakeri; Zahra Meshkat. "Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran". Iranian Journal of Pathology, 12, 3, 2017, 248-256.
Rostami, S., Pasdar, A., Gerayli, S., Hatami, H., Sepahi, S., Nategh, F., Meshkat, M., Hoseini, S., Ahadi, M., Sima, H., Vosughinia, H., Sarvghad, M., Esmaeelzade, A., Nomani, H., Mosanan Mozafari, H., Rezai Talab, F., Shakeri, M., Meshkat, Z. (2017). 'Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran', Iranian Journal of Pathology, 12(3), pp. 248-256.
Rostami, S., Pasdar, A., Gerayli, S., Hatami, H., Sepahi, S., Nategh, F., Meshkat, M., Hoseini, S., Ahadi, M., Sima, H., Vosughinia, H., Sarvghad, M., Esmaeelzade, A., Nomani, H., Mosanan Mozafari, H., Rezai Talab, F., Shakeri, M., Meshkat, Z. Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran. Iranian Journal of Pathology, 2017; 12(3): 248-256.

Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran

Article 9, Volume 12, Issue 3, Summer 2017, Page 248-256  XML PDF (427 K)
Document Type: Original Research
Authors
Sina Rostami1; Alireza Pasdar2, 3; Sina Gerayli4; Hamed Hatami5; Samaneh Sepahi6; Fatemeh Nategh7; Mojtaba Meshkat8; Seyed Mousalreza Hoseini9; Mitra Ahadi9; Hamid Reza Sima10; Hasan Vosughinia10; Mohammad Reza Sarvghad11; Abbas Esmaeelzade9; Hosein Nomani12; Homan Mosanan Mozafari9; Fariba Rezai Talab10; Mohammad Taghi Shakeri13; Zahra Meshkat 12
1Dept. of Laboratory Medicine, Children's and Women's Health, NTNU - Norwegian University of Science and Technology, NO-7491 Trondheim, Norway
2Dept. of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK
4Dept. of Biology, University of Western Ontario, London, Ontario N6A5BF, Canada
5Dept. of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6Targeted Drug Delivery Research Centre, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
7Applied Biotechnology Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
8Islamic Azad University, Mashhad Branch, Mashhad, Iran
9Dept. of Internal Medicine, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
10Dept. of Internal Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
11Dept. of Infectious Diseases, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
12Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
13Dept. of Biostatistics, Public Health School, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Background and Objectives: Interferon-gamma is an important cytokine, which facilitates immunity against intracellular pathogens. Several factors, including genetic variations of cytokine-producing genes have been shown to influence the progression and severity of Hepatitis C virus (HCV) infection.
Methods: Between January and December 2012, 87 HCV-infected individuals and 89 individuals without HCV infection were recruited for the study of Single Nucleotide Polymorphism (SNP) at Interferon Gamma (IFNG) +874 T/A. After extraction of genomic DNA from Peripheral Blood Mononuclear Cells (PBMCs) in blood sample of the individuals, Amplification Refractory Mutation System (ARMS) polymerase chain reaction was performed to evaluate the SNP at this position.
Results: The frequency of genotype TA was 62.1% in the HCV-infected group, while it was 47.2% for the control group (p=0.033). However, after adjusting for confounders (including alcohol consumption, drug addiction, transfusion, and tattoos), the genotypes at this position did not show any statistically significant association with HCV infection (adjusted P values were above 0.05). The frequency of allele A was slightly higher in patients than the controls (55.2% versus 48.3%).Carriers of A allele were more frequent in patients with HCV infection compared to the control group (55.17% in patients versus 48.31% in the control group; P=0.02). However, after adjustment for confounders, the results were no longer statistically significant (P=0.2).
Conclusion: A carrier status for certain alleles and genotypes at Interferon Gamma (IFNG) +874 T/A may lead to higher susceptibility to HCV infection in a certain population.
Keywords
Interferon-gamma; single nucleotide polymorphism; Hepatitis C Virus
Main Subjects
Microbiology
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