Garlic Extract Can Induce Apoptotic Cell Death in The Human Colon
Adenocarcinoma HT29 Cell Line
Volume
5
(2010) Issue
3 (Summer )
Original Article
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KeyWords:
Garlic , HT29 cell , Apoptosis , TUNEL
Authors:
Alireza Azizzadeh Delshad, Marjan Heshmati, Mohammad Hossein Ghaini
Abstract:
Background and Objective: Garlic has been known worldwide as a dietary constituent
with many pharmacological effects. The present in vitro study was designed to investigate the
putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis
induction.
Material and Methods: Human colon adenocarcinoma cells HT29 were treated with different
doses (1, 5 and 10 mg/ml) of fresh garlic extract in cell culture at 24, 48 and 72 h. Cell death was assessed
by viability test, and its apoptotic nature was determined by terminal deoxynucleotidyltransferasemediated
dUTP nick end labeling (TUNEL) assay. All experiments were statistically analyzed using
SPSS 11.5 software
Results: Garlic extract induced a dose-dependent cell death with the highest values at 72 hours.
The apoptosis analyses by TUNEL assay demonstrated that in each dose/time group the apoptotic
values are much less prominent than the related cell death percentage found in viability test. In
every dose, the sum of apoptotic cells in all three-time points was nearly equal to the percentage of
cell death at 72 hours.
Conclusion: Fresh garlic extract can induce a dose-dependent apoptotic cell death in cultured
HT29 cell line.
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