Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Relationships of Serum Level of High- Sensitivity C- Reactive Protein (hs- CRP) and White Blood Cell (WBC) Count with Gamma- Glutamyltransferase among Iranian Healthy Adults2092148337ENAlireza AbdollahiDept. of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran0000-0002-5714-967XSaeed ShoarDevelopment Association for Clinical Study (DACS), Student Scientific Research Center (SSCR), Tehran University of Medical Sciences, Tehran, IranJournal Article20110913<em>Background & Objective:</em> There are many common infections and inflammations among people over the world. This demands an affordable and reliable test with high sensitivity/specificity to predict or confirm the diagnosis of such cases in routinely clinical practice. In the present study, we aimed to find any potential correlation between serum levels of GGT and CRP- Q and/or hs- CRP and WBC count as the well- known markers of inflammation in human body.
<em>Materials and Methods:</em> Through a cross- sectional study, serum levels of CRP-Q, hs- CRP, and GGT, in addition to WBC count were measured in 1,500 healthy people, referred to Blood Transfusion Organization from across the country. Data were analyzed after the patients were checked by physical examination for ruling out the presence of any inflammation or other illness. Serum levels of GGT, CRP- Q, hs- CRP, and WBC count were analyzed by SPSS for windows version 16.
<em>Results:</em> Analysis showed a positive linear correlation between CRP- Q and hs- CRP with GGT also confirmed by non- parametric tests.
<em>Conclusion:</em> Serum GGT may be an inflammatory index and a useful marker in approaching to inflammatory diseases.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Immunohistochemical Expression of p53 and bcl2 in Colorectal Adenomas and Carcinomas Using Automated Cellular Imaging System2152238338ENBan QasimDept. of Pathology and Forensic Medicine, Al-Nahrain University, Baghdad, IraqHusam AliDept. of Pathology and Forensic Medicine, Al-Nahrain University, Baghdad, IraqAlaa HusseinDept. of Pathology and Forensic Medicine, Al-Nahrain University, Baghdad, IraqJournal Article20140908<em>Background & Objective:</em> The current approaches to reduce the risk of colorectal carcinoma are through the detection and removal of the precursor lesion” adenomatous polyps”. The study was conducted to evaluate the immunohistochemical expression of p53 and bcl2 in colorectal adenomas and carcinomas.
<em>Patients and Methods:</em> A total of 86 cases, 33 colorectal adenomas, 33 colorectal adenocarcinomas and 20 samples of non -tumerous colonic tissue as control, were included in this retrospective study. Sections were stained immunohistochemically for p53 and bcl2. Scoring was performed using Digimizer software. Data were analyzed using SPSS program (Statistical Package for Social Sciences) version 16 and Microsoft Office Excel 2007.
<em>Results:</em> The frequency of p53 positive cases was significantly higher in carcinoma than adenoma while the frequency of bcl2 positive cases was significantly higher in adenoma than carcinoma. P53 expression was significantly higher in large sized adenomas, villous configuration, severe dysplasia, and multiple lesions. Bcl2 expression showed significantly correlated with adenomas of small size, solitary, tubular, and mild dysplasia. There was a significant correlation between bcl2 expression and non-mucinous carcinoma and a negative correlation with tumor size.Therewas an inverse relationship between bcl2 and p53 expression in both colorectal adenomas and carcinomas.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Chemotherapy and Management of Locally Advanced Carcinoma Cervix2242308339ENRajyashri SharmaDepartment of Gynaecology, Jawaharlal Nehru Medical College, Aligarh Muslim University,Aligarh (UP), IndiaHuma FirdausDepartment of Gynaecology, Jawaharlal Nehru Medical College, Aligarh Muslim University,Aligarh (UP), IndiaVeena MaheshwariDepartment of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh (UP), IndiaKafil AkhtarDepartment of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh (UP), IndiaShahid Ali SiddiquiDepartment of Radiotherapy, Jawaharlal Nehru Medical College, Aligarh Muslim University , Aligarh (UP), IndiaJournal Article20110525<em>Background & Objective:</em>the present study was undertaken to ascertain the incidence of early, advanced cancer cervix and its recurrence and role of chemotherapy in locally advanced cancer cervix.
<em>Material and Methods:</em> The present study was conducted in the Departments of Obstetrics & Gynaecology, Pathology and Radiotherapy and the specimens examined in the Department of Pathology of Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India from July 2009 to July 2010.One hundred histologically confirmed carcinoma cervix cases were divided into two groups. I: Study group of cases receiving different types of chemotherapy and II: Control group comprised of cases on pre and postoperative radiotherapy and surgery. Patients were staged and locally advanced inoperable cases (Stage IB-IVA) were given different types of chemotherapy. Thereafter subjective and objective assessment was made according to World Health Organization (WHO) criteria of tumor response.
<em>Results:</em> Majority of cases (60.0%) presented in stage IIB of disease by International Federation of Gynecology and Obstetrics (FIGO) classification. Commonest symptom was postmenopausal vaginal bleeding, 70.0% cases. 68.0% & 56.0% cases showed complete response (CR) on chemotherapy and radiotherapy respectively. Stage IIB patients showed the best response with CR in 70.0% & partial response (PR) in 16.7% cases. Colposcopic evaluation on 12 -18 months follow up showed 08 to be disease free, with decrease in size of tumor.
<em>Conclusion:</em> Chemotherapy is an effective mode of therapy and can be considered as an adjunct to surgery or radiotherapy to improve the overall survival of cancer cervix patients.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Immunofluorescence Pattern of Autoimmune Bullous Diseases in Iranian Patients2312358340ENAlireza MonsefDept. of Pathology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran0000-0002-4623-2988Mahmood FarshchianDept. of Dermatology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, IranMohammad JafariDept. of Pathology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, IranMehdi FarshchianDept. of Dermatology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, IranJournal Article20111106<em>Background & Objective:</em> Autoimmune bullous diseases are associated with autoimmunity against structural components in the skin and mucous membranes. Autoantibodies are against the intercellular junctions in pemphigus disease and hemidesmosomal unchoring complex in pemphigiod diseases and epidermolysis bullosa aquisita. The tissue-bound and circulating serum autoantibodies can be detected with direct immunofluorescence (DIF) and indirect Immunofluorescence (IIF) tests. The aim of this study was to pinpoint the immunofluorescence pattern of Iranian patients with autoimmune bullous diseases.
<em>Methods:</em> In a prospective case series study, sixteen patients with autoimmune disease enrolled in the study for two years. Perilesional skins and sera from the patients were used in DIF and IIF for detection of immunofluorescence pattern.
<em>Results:</em>Out of 16 cases, 9 cases had pemphigus and 7 cases had bullous pemphigoid. All cases of pemphigus had positive DIF in intercellular region with lacelike pattern; IgG was detected in all cases, IgA in 1(11.1%) case and C<sub>3</sub> in 3 (33.3%) cases. One (11.1%) case of pemphigus had positive IIF in intercellular region with lacelike pattern; circulating autoantibodies were IgG and IgA. All cases of bullous pemphigiod had positive DIF in dermal-epidermal Junction with linear pattern. IgG was detected in all cases, IgM in one (14.3%) case, and C3 in six (85.7%) cases. One (14.3%) case of bullous pemphoid had positive IIF in dermal-epidermal Junction with linear pattern; circulating autoantibody was IgG.
<em>Conclusion:</em> Immunofluorescence tests are sensitive diagnostic methods for autoimmune bullous diseases. IIF positive cases in our study were lower compared to the previous reports.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Effect of Low Level Laser Therapy on the Surgical Wound Healing in Hamster22362408341ENNeda NasirianDept. of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran0000-0002-2554-7702Ali NasirianDept. of Pathology, Qazvin University of Medical Sciences, Qazvin, IranJournal Article20111214<em>Background and Objectives</em><em>:</em> Accelerating wound healing is a very important factor for patients to return to ordinary life. Laser seems to have positive effect on cutaneous wound healing. This study aimed to investigate the effect of red light laser 630 nm on cutaneous surgical wound in hamster and to compare outcomes such as angiogenesis, number of fibroblasts and collagen formation in intervention and control group.
<em>Material</em><em>s and Methods:</em> This experimental study carried out with collaboration of Qazvin University of Medical Sciences with Mehregan Pet Clinic in winter of 2009. Thirty suri hamsters were randomly allocated in two groups and four parallel wounds were made on their backs. The intervention group was radiated with red-light laser 630 nm. After 2,5,10 and 14 days, skin biopsy were obtained and number of blood vessels, fibroblast and collagen production were compared with the control group. Data were analyzed using independent sample student <em>t</em> test using SPSS version 10 and <em>P </em>value less than 0.05 was considered significant.
<em>Result:</em> Evaluation of wounds in experimental and control groups showed significantly increased number of vessels and fibroblasts as well as collagen production in laser radiating group (<em>P</em><0.05).
<em>Conclusion:</em> Laser therapy (630 nm) can accelerate wound healing in comparison with control group.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Frequency of Nosocomial Infections with Antibiotic Resistant Strains of Acinetobacterspp. in ICU Patient2412458342ENMaryam AminiDept. of Infectious Diseases and Tropical Medicine, Shahed University, Tehran, Iran0000-0002-9463-6952Ali DavatiDept. of Social Medicine, Shahed University, Tehran, IranMahdieh GolestanifardDept. of Infectious Diseases and Tropical Medicine, Shahed University, Tehran, IranJournal Article20120111<em>Background and Objective:</em><em>Acinetobacter</em> spp.a Gram-negative coccobacillus is increasingly reported as important cause of nosocomial infections. Multi-drug resistance (MDR) of<em>Acinetobacter</em>spp., raises concerns over our ability to treat serious infections with these organisms. The aim of this study was to determine the frequency and associated risk factors for infections with MDR <em>Acinetobacter </em>spp. in ICU patients ofShahid Mostafa Khomeini Hospital, Tehran, Iran.
<em>Patients and Methods:</em>This descriptive-analytical andcross-sectional study was designed in 3 years period from April 2008 to March 2010 on 130 patients. For bacteriological analysis, sputum<em>,</em> blood<em>,</em> urine and wound specimens were used from patients within >48 hr after admission. Patient’s clinical and epidemiologic data were collected<em>,</em> from recordedfile<em>,</em> and correlated to <em>Acinetobacter </em>spp. infection. The data were analyzed using SPSS16 statistical software,chi-square, and Mann-Whitney test.
Results: The frequency of <em>Acinetobacter</em>spp. infection separately by years was 21.5%, 30.8% and 47.7% in 2008, 2009, 2010, respectively. All isolateswere resistant to <em>carbnicillin,</em><em> piperacillin, cefotaxime </em>and<em> cephalotin</em>, 99.2% to <em>ciprofloxacin</em><em>, cotrimoxazole </em>and<em> chloramphenicol</em>, 97.7% to <em>imipenem</em>, 95.4% to <em>tetracycline </em>and 91.5% to <em>gentamicin</em>. The highest sensitivity was to <em>amikacin</em> 14.6%.
<em>Conclusion:</em>Nosocomialinfections with <em>Acinetobacter </em>spp. during the three years<em>,</em> was a growing trend, and all isolates were MDR and highest susceptibility was to <em>Amikacin. </em>Most important risk factors wereincorrect diagnosis<em>,</em> inappropriate usage<em>,</em> doses, andtime of antibiotic therapy, inappropriate formulation of some antibiotics.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Inguinal Lymph Node Metastasis as the First Presentation of Endometrial Carcinoma2462508343ENShokouh Aghipour ZahirDept. of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, IranKiyanoosh SedaghatDept. of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, IranSaeed KargarDept. of Surgery, Shahid Sadoughi University of Medical Sciences, Yazd, IranJournal Article20110907 Endometrial adenocarcinoma is uncommon in fewer than 40-year-old people. The first common sign and symptoms are abnormal vaginal bleeding and discharge. Metastasis is occurred in the late stage. Here we present a 31-year-old nullipar woman who was admitted to surgical ward with enlarged right inguinal lymphnode from one month ago. She had no history of previous malignancy, infectious condition, vaginal bleeding, and discharge. Abdominal sonography revealed no abnormality. She underwent surgical biopsy and metastatic adenocarcinoma most suspected from genital tract was reported. Based on pathological recommendation diagnostic curettage was performed and endometrial adenocarcinoma of conventional type as a first origin was confirmed. To our knowledge metastasis to inguinal lymph node as a first manifestation of endometrial carcinoma is rare.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Kimura’s Disease: A Case Report and Review of Literatures2512558344ENJahanbanoo ShahryariDept. of Pathology, Kerman University of Medical Sciences, Kerman, IranShahriar DabiriDept. of Pathology, Kerman University of Medical Sciences, Kerman, Iran0000-0002-5922-3976Amin TalebiDept. of Pathology, Kerman University of Medical Sciences, Kerman, IranJournal Article20111231Kimura’s disease (KD) is a chronic inflammatory disorder primarily seen in male Asians during the second and third decades of life. Clinically, it presents as solitary or multiple subcutaneous nodules, predominantly in the head and neck region, typically in the pre auricular region, forehead, and scalp. The etiology of Kimura disease is still unknown. This disorder should be suspected when the clinical triad of painless unilateral cervical adenopathy, hypereosinophilia, and hyper-IgE is present. We report a case of KD with multiple subcutaneous nodules in the parotid, submandibular and posterior auricular regions, hypereosinophilia and hyper IgE levels.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Asymptomatic Bronchogenic Cyst in a 35-Year-Old Woman as an Incidental Finding2562588345ENSepideh SiadatiDept. of Pathology, Shahid Beheshti Hospital, Babol University of Medical Sciences, Babol, IranSeyed Reza ModaresDept. of Surgery, Shahid Beheshti Hospital, Babol University of Medical Sciences, Babol, IranJournal Article20120111Bronchogenic cyst is a result of abnormal development of the ventral foregut presents as a discrete mass. Most commonly, it is appeared in the middle mediastinum and rarely is seen in adults. We report a 35-year-old woman who was asymptomatic and incidentally was found with a mass in chest radiograph.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Primary Subcutaneous Hydatid Cyst in Scapula2592618346ENTahereh MirzaeiDept. of Parasitology, Kashan University of Medical Sciences, Kashan, IranHossein HooshyarDept. of Parasitology, Kashan University of Medical Sciences, Kashan, IranJournal Article20120116Hydatid disease, caused by larval stage of <em>Echinococcus granulosus</em>, is a common parasitic infection of human and animal. Although liver and lung are the most commonly affected area, hydatid cyst may develop any part of the body. Primary subcutaneous hydatid cyst is extremely rare. We report a case of 54 years old man who presented with palpable mass in scapular region from 3 years ago. Present and past medical history was not significant finding. Pathologic evaluation identified the cystic structure as unilocular hydatid cyst. Primary hydatid disease constitutes a potentially serious differential diagnosis of any subcutaneous mass in endemic area of hydatid disease.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Molecular Genetics Diagnosis of Wilson Disease: the First Reported Case of ATP7BGene Mutation at Codon 778 in Southwest Iran2622668347ENHamid GalehdariDept. of Genetics, Shahid Chamran University, Ahvaz, IranRaheleh TangestaniToxicology Research Centre, Jundishapur University of Medical Science, Ahvaz, IranJournal Article20120123Wilson disease is a metabolic disorder with an autosomal recessive genetic pattern and occurs in 1-4 of every 100000 individuals. Inactivation of the <em>ATP7B</em> gene leads to accumulation of the toxic copper to liver and brain causing hepatic and neurological complication. Therefore, most patients suffer from chronic hepatic inflammation and central nervous system disorder. Nowadays, up to 500 mutations were found in the <em>ATP7B</em> gene that weaken or fully disrupt the function of the gene product. Recurrent mutations were found in different population. We found a homozygous pathogenic missense mutation at codon 778 (R778W) in an individual from southwest Iran. This mutation has been reported in previous studies in the continents America and Europe. The present study is the first report from Wilson disease that has been diagnosed in southwest Iran. This mutation has been shown in previous studies in patients from continents America and Europe.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53037420120901Beta-Thalassemia Carrier Detection by NESTROFT2672678348ENSomsri WiwanitkitWiwanitkit House, Bangkhae, Bangkok, ThailandViroj WiwanitkitWiwanitkit House, Bangkhae, Bangkok, ThailandJournal Article20140908