Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Parasitemia and Hematological Alterations in Malaria: A Study from the Highly Affected Zones188325ENSethi BhawnaDept. of Pathology, HNB Garhwal University, Srinagar, Uttarakhand, IndiaArora BhartiDept. of Microbiology, MAMC,Agroha,Hissar, IndiaKumar YogeshDept. of Ophthalmology, HNB Garhwal University, Srinagar, Uttarakhand, IndiaAggarwal ReenaDept. of Pathology, Swami Dayanand Hospital, Shadhara, New Delhi, IndiaJournal Article20120306<em>Background</em><em>& Objectives</em><em>:</em> Inspite of intensive worldwide efforts to reduce its transmission, malaria remains the most serious and widespread protozoal infection of humans. It is a protozoan disease transmitted by the bite of infected female anopheles mosquito. Malaria has long featured prominently in the grey area between parasitology and hematology. This study has been performed to evaluate various hematological alterations in patients infected with malaria and to add more detailed information, especially from these highly affected zones.
<em>Materials and methods</em><em>:</em> A two-year, hospital-based study was conducted and hematologic profiles of 200 persons infected with malaria (<em>Plasmodium vivax</em><em> or </em><em>Plasmodium falciparum</em>) infection, were examined and the results were compared with standard normal values.
<em>Results</em><em>:</em> One-hundred-ninety-six patients were infected with <em>Plasmodium vivax</em><em>, three </em>with <em>Plasmodium falciparum</em>, and one <em>was infected with both. </em>Patients with parasitemia tended to have significantly lower platelets, hemoglobin, white blood cell and red blood cell counts, blood indices and hematocrit than normal. Thrombocytopenia is identified as a key indicator of malaria in these febrile patients.
<em>Conclusion:</em> Anemia and thrombocytopenia are the classical changes. Changes in the white blood cells are less dramatic, may vary due to variable size and type of cases, variability of the species, and geographical differences.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Effects of Resistance Training on Serum Cortisol and Dehydroepiandrosterone Levels in Trained Young Women9168326ENHamid Agha-AlinejadDept. of Exercise Physiology, Physical Education and Sports Sciences, Tarbiat Modares University, Tehran, IranMohammad-Ali KohanpourDept. of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Tehran, IranSuzan SanaviClinical Dept, University of Social Welfare & Rehabilitation Sciences, Tehran, IranSoghra SojudiDept. of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Tehran, IranGholam-Reza BehrouziRojan Azma Mfg, Alborz, IranMona MirsepasiDept. of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Tehran, IranJournal Article20120424<em>Background & Objectives:</em>Endocrine hormones, through their anabolic or catabolic function, contribute in body homeostasis. They can be used to assess the physical performance in athletes. This study was performed to determine the resistance training (RT) influences on serum cortisol and dehydroepiandrosterone in trained young females.
<em>Materials & Methods:</em>The study population consisted of 36 women (20-25 years) who were divided into 2 experimental groups (A, B) and control group (C). Experimental groups were scheduled to do 8-week incremental RT program, intermittently (A) or continuously (B), through alterations in the prescribed training velocity. Two days before starting and after ending of program, in a testing session of RT with the intensity of 20% one repetition maximum strength, blood samples for measuring cortisol and dehydroepiandrosterone were obtained (a pre-post test design). The SPSS version 16 was used for statistical analyses.
<em>Results:</em> Serum cortisol of experimental groups showed significant decline (<em>P</em>=0.002 in each group) without significant difference between two groups (<em>P</em> =0.819), while, DHEA and DHEA/Cortisol ratio had significant increase (all <em>P</em> =0.000) with no significant difference between two groups (<em>P</em><sub> DHEA</sub>=0.275 and <em>P</em> <sub>ratio</sub>= 0.998) at both stages.
<em>Conclusion:</em> With considering the role of RT in establishing an anabolic status following exercise, it seems that young women can use different types of RT to improve their physical performance. This conclusion needs more researches regarding RT
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101The Frequency of HPV 16 and 18 in Cervical Discharge by PCR in Women with Abnormal Pap Smear or Biopsy17208327ENMajid SharbatdaranDepartment of Pathology, Babol University of Medical Sciences, Babol, IranSepideh SiadatiDepartment of Pathology, Babol University of Medical Sciences, Babol, IranMahtab ZeinalzadehDepartment of Gynecology and Obstetrics, Babol University of Medical Sciences, Babol, IranShahriar ShafaeiDepartment of Pathology, Babol University of Medical Sciences, Babol, IranZahra BasiratDepartment of Gynecology and Obstetrics, Babol University of Medical Sciences, Babol, IranAmir EsmiDepartment of Pathology, Babol University of Medical Sciences, Babol, IranJournal Article20120308
Background: Cervical cancer is the second most common cancer in the world among women and human papilloma virus (HPV) plays a major role in its development. The aim of this study was to determine the frequency of HPV type 16 and 18 in cervical discharge by polymerase chain reaction (PCR) method in women with atypical biopsy or papsmear.
Method: This case- control study was performed on women in Yahyanejad Hospital, Babol University of Medical Sciences, Babol, Iran during 2008-2009. Sixty women with normal papsmear (group1) and 30 women with atypical papsmear or biopsy (group 2) were enrolled in the study and their cervical discharge was assessed for HPV type 16 and 18. Data was analyzed with SPSS, Chi-Square, Fisher<sup>,</sup>s Exact test and t-test and <em>P</em><0.05 was considered significant.
Results: HPV type 16 was founded in 10% women of group 2 but not seen in group1. HPV 18 was not detected. All women had one partner and none of them had alcohol consumption.
Conclusion: In comparison with other studies, the frequency of HPV infection is lower in our study. We considered this is strongly related to our culture and religious beliefs.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Determination of the Resistance Pattern of Prevalent Aerobic Bacterial Infections of Diabetic Foot Ulcer21268328ENMaryam AminiDepartment of Infectious Diseases and Tropical Medicine, Shahed University, Tehran, Iran0000-0002-9463-6952Ali DavatiDepartment of Social Medicine, Shahed University, Tehran, IranMasomeh PiriDepartment of Infectious Diseases and Tropical Medicine, Shahed University, Tehran, IranJournal Article20120404<em>Background and Objective:</em> Diabetes mellitus is one of the main problems in health systems in the world. Diabetic Foot infections (DFI) is one of the main complications and the most cause of non-traumatic lower limb amputation .This study aimed to determine the prevalence of bacteria involved in DFI and their antibiotic resistance in patients with DFI diagnosis.
<em>Patients and Methods:</em> This descriptive-analytical and cross-sectional study was designed in 4 years period from April 2007 to March 2010 on 90 patient in Shahid Mostafa Khomeini Hospital,Tehran, Iran .For bacteriological analysis, all wound samples culture grown by standard methods bacteriology and Disk diffusion method was used for antibiogram. Patient’s clinical and epidemiologic data were collected from recorded file. The data were analyzed by using SPSS16 statistical software.
<em>Results:</em> 104 bacteria were isolated from 90 patients. 57.70% were gram-positive and 42.30% were gram-negative. Among gram-positive bacteria <em>Staphylococcus aureus</em> (60%) and <em>enteroccoci spp.</em>(33.3%) and among gram-negative bacteria <em>E. coli</em> (47.73%), <em>Pseudomonas Aeroginosa</em> (22.73%) and <em>Proteus</em><em> spp</em>.(18.18%) were the most common isolates respectively. 75% of isolates were resistant to two antibiotics or more. Previous antibiotic therapy was significant risk factor for multidrug resistant (MDR) infections (<em>P</em>: 0.003). All gram-positive isolates were sensitive to <em>Vancomycin</em>. <em>Imipenem</em> and <em>Amikacin</em> had good activity against gram-negative bacteria.
<em>Conclusion:</em>Infection with MDR bacteria in patients with diabetic foot ulcers is high and have significant association with recent antibiotic therapy. So the proper use of antibiotics in order to prevent the creation of multi-drug resistant bacteria is recommended.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Expression of Anaplastic Lymphoma Kinase Protein in Human Breast Cancer27358329ENAli Zare MehrjardiDept. of Pathology, Firouzgar Hospital, Tehran University of Medical Sciences, Tehran, IranAmineh VaghefiDept. of Pathology, Firouzgar Hospital, Tehran University of Medical Sciences, Tehran, IranJournal Article20140908Background & Objectives: Anaplastic lymphoma Kinase (ALK) is a receptor tyrosine kinase involved in the genesis of several human cancers. ALK was initially identified because of its involvement in anaplastic large cell lymphoma (ALCL). ALK is believed to foster tumorigenesis following activation by autocrine and/or paracrine growth loops. Studies reveal that the presence of anti-ALK antibodies may be relevant to the relatively good prognosis. Therapeutic approaches consisting of gene therapy and immunotherapy targeting this molecule hold promise.
Material & Methods: We examined a number of human breast cancers to see if ALK is expressed in this tumor and studied its relation with type of carcinoma and its grade, tumor size, presence of necrosis, vascular invasion , skin involvement, lymph node metastasis and patient’s age.
Result: 100 patients were enrolled with mean age of 50.2 ± 12.5 years. The histological phenotypes of the breast cancers studied included Invasive Ductal Carcinoma, Invasive Lobular Carcinoma and Medullary Carcinoma. ALK expression was evaluated by immunohistochemistry which was positive in 47 cases (47%). No statistically significant relationship is found between the above mentioned parameters except for tumor size and ALK expression. (<em>P</em>< 0.01)
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Development of 16S rRNA targeted PCR methods for the detection of Escherichia coli in Rainbow trout (Oncorhynchus mykiss)36448330ENFatemeh Fattahiepartment of Fishery and Environment, Faculty of Natural Resources, University of Tehran, Karaj, IranAlireza MirvaghefiDepartment of Fishery and Environment, Faculty of Natural Resources, University of Tehran, Karaj, IranHamid FarahmandDepartment of Fishery and Environment, Faculty of Natural Resources, University of Tehran, Karaj, IranGholamreza RafieeDepartment of Fishery and Environment, Faculty of Natural Resources, University of Tehran, Karaj, IranAlireza AbdollahiDepartment of Pathology, Tehran University of Medical Sciences, Tehran, Iran0000-0002-5714-967XJournal Article20120907
<em>Objectives</em>: The presence of <em>E.coli</em> in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. The objective of this study was to develop an improved PCR method based on species – specific 16 S rRNA gene primers (FES, RES) for detection of <em>E. coli</em> from agar plates and fish tissues.
<em>Materials and Methods:</em> In this study, For the rapid detection of <em>E .coli</em> from fish a set of primers (FES, RES), targeting 16S rRNA gene sequences of the specific microorganism was designed, and fifty two rainbow trout were obtained from Karaj fish farm. Then 1mL of bacterial concentration of 10<sup>6</sup>CFU/ml was injected into intraperitoneal cavity. Samples were collected from liver and kidney after 48h injection. The PCR reaction conditions were optimized to permit detection of organism from agar plates and fish tissue in a day.
<em>Results:</em> All tissue samples were positive for microbiological and PCR identification. DNA was successfully extracted by a boiled – extraction method or by phenol – chloroform – isoamyl alcohol. The BLAST analysis from sequencing of 4 amplicons randomly selected showed similar results, with the match being <em>E .coli</em> with a 100% similarity (not shown here).
<em>Conclusion:</em> It is concluded that this method is fast, specific and sensitive to detect<em> E.coli</em> in infected and asymptomatic animals, fish product, and may have a positive impact on public and environmental health.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101A Pedunculated Hamartomatous Polyp of the Palatine Tonsil45478331ENRaphael Hart LyngdohDepartment of Pathology, Father Muller’s Medical College, Mangalore, IndiaSumanth DevarajuDepartment of Pathology, Father Muller’s Medical College, Mangalore, IndiaLeena JBDepartment of Pathology, Father Muller’s Medical College, Mangalore, IndiaJournal Article20120202
Hamartomatous polyps of the palatine tonsil are very rare. They have been variously termed as a lymphangiectatic fibrous polyp, lipoma, pedunculated tonsil etc. in the English literature. We present here a case of hamartomatous polyp of the tonsil occurring in a 32-year-old male who presented with bilateral enlarged palatine tonsils with recurrent tonsillitis. Surgical excision showed two pale brown nodular tissue bits, larger measuring 4x3x1 cm and smaller measuring 3.5x2.5x1.5 cm. An irregular pedunculated polypoidal tissue measuring 1.5x1x0.8cm was seen attached to the smaller mass, the cut surface of which showed pale white, lobulated areas. On histological examination, the polyp showed lining by stratified squamous epithelium and was composed of fibrocollagenous stroma with many dilated blood vessels, adipocytes, subepithelial lymphoid aggregates and benign mucinous glands suggestive of a hamartomatous polyp. Both the tonsils showed features of chronic tonsillitis.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Bilateral Multiple Lymphoepithelial Cysts of Palatine Tonsils48548332ENZhaleh Mohsenifar1Department of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, IranNazanin MahdaviDepartment of Oral and Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences, Tehran, IranSara BagheriDepartment of Oral and Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences, Tehran, IranJournal Article20120207
Lymphoepithelial cyst of oral cavity is a rare cystic lesion that presents as an asymptomatic submucosal mass and is usually discovered during routine dental examinations. The site most commonly affected is floor of the mouth and tonsillar involvement seems to be very rare. Multiple Lymphoepithelial cysts have been reported in parotid of HIV positive patients however in oral cavity they usually present as solitary lesions.We report a case of multiple bilateral Lymphoepithelial cysts of the palatine tonsils in a 72 years old male that presents with fever, pain and obstructive sleep apnea.We didn’t find any bilateral or multiple tonsillar lymphoepiyhelial cysts in the literature and the present paper seems to be the first report. We suggest although lymphoepithelial cysts rarely occur in the tonsils but they should be considered in differential diagnosis of adenotonsillar enlargement and related obstructive sleep apnea.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Splenogonadal Fusion Associated with Bilateral Cryptorchidism, Presenting as an Operative Surprise - Report of a Case55588333ENMaryam AbolhasaniOncopathology Research Center, Tehran University of Medical Sciences, Tehran, Iran0000-0002-3046-5429Mojgan AsgariHasheminejad Clinical Research Developing Center (HCRDC), Tehran University of Medical
Sciences, Tehran, IranHossein KeymoosiDepartment of Pathology, Tehran University of Medical Sciences, Tehran, IranJournal Article20120404Splenogonadal fusion is a rare entity with approximately 150 reported cases until the year 2005. The entity is a rare congenital anomaly in which there is fusion of splenic and gonadal anlagen or mesonephric derivatives. Splenogonadal fusion has two continuous and discontinuous types. About ten cases have been reported to be associated with bilateral cryptorchidism in literature. Very few cases have been diagnosed preoperatively. Many cases present as a testicular swelling and undergo an unnecessary orchiectomy with the suspicion of a testicular neoplasm. Herein, we report a new case of discontinuous splenogonadal fusion in a 29 years old man with bilateral cryptorchidism who underwent surgery. Surprisingly two masses were noted adjacent to undescendent testis in left groin in operative room which were resected and proved to be a new case of splenogonadal fusion in histological exam.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Superficial Acral Fibromyxoma: a Rare Tumor Diagnosed by Cytology59638334ENAnuradha SinhaDepartment of Pathology, Bankura Sammilani Medical College, Bankura, West Bengal, IndiaJyoti Prakash PhukanDepartment of Pathology, Bankura Sammilani Medical College, Bankura, West Bengal, IndiaGautam BandyopadhyayDepartment of Pathology, R.G. Kar Medical College, Kolkata, West Bengal, IndiaSanjay SenguptaDepartment of Pathology, Bankura Sammilani Medical College, Bankura, West Bengal, IndiaRinki SahaDepartment of Ophthalmology, KPC Medical College, Kolkata, West Bengal, IndiaJournal Article20120306Superficial acral fibromyxoma are rare soft tissue tumors predominantly involving hands and feet of adults. There are only a few reported cases of this benign neoplasm, but no FNAC diagnosis of this tumor is reported in the literature. A Forty-eight year old female presented with a 5 x 3.5 cm globular, firm swelling over the right fifth toe. Fine needle aspiration cytology (FNAC) of the lesion was interpretated as superficial acral fibromyxoma, <span style="text-decoration: line-through;">and </span>Subsequent histopathology confirmed the diagnosis and demonstrated cytohistological correlation for the first reported time in the literature. SAFM may recur if not adequately excised. Preoperative FNAC diagnosis will help the surgeons to plan for wider excision to prevent recurrence. Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Low Grade Endometrial Stromal Sarcoma: Report of a Rare Uterine Malignancy64708335ENPinki PandeyDepartment of Pathology, M M Institute of Medical Sciences and Research, M M University, Haryana, IndiaAlok DixitDepartment of Pharmacology, M M Institute of Medical Sciences and Research,
M M University, Haryana, IndiaAparna TanwarDepartment of Pathology, M M Institute of Medical Sciences and Research,
M M University, Haryana, IndiaNanak Chand MahajanDepartment of Pathology, M M Institute of Medical Sciences and Research, M M University, Haryana, IndiaJournal Article20120204Endometrial stromal sarcoma represents a very rare pathological entity occurring as a malignant disease in women genital sphere, frequently in the age group of 40-50 years. Low grade endometrial stromal sarcoma (LGESS) is a rare uterine sarcoma constituting 0.2% of all the uterine malignancies with a good prognosis despite a tendency to recur. We report a case of 42 years old woman presented with menorrhagia, dysmenorrhoea, anemia and rapidly enlarging mass in uterus clinically diagnosed as leiomyoma, underwent a hysterectomy. Histological examination revealed cells with ovoid to short, spindle shaped nuclei resembling endometrial stromal cells proliferating in a space occupying manner and compressing and partially infiltrating the myometrium, rendered the diagnosis of LGESS. Our case emphasizes the inevitability for high index of skepticism and proper preoperative diagnosis in this rare tumor.
Farname Inc in collaboration with Iranian Society of PathologyIranian Journal of Pathology1735-53038120130101Isolated Splenic Metastasis Presenting with Continuous Fever and Abdominal Pain71718336ENKamon ChaiyasitNutritional Clinic, Maefahlaung University Hospital, Bangkok, ThailandViroj WiwanitkiThai POCT forum coordinator, Bangkok, ThailandJournal Article20140908