TY - JOUR ID - 8895 TI - Relationship between grade and MDM2 oncoprotein overexpression in transitional cell carcinoma of the urinary bladder JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Jalali Nadoushan, Mohammad Reza AU - Ghorbanian, Ehsanollah AU - Taheri, Touraj AD - Dept. Pathology, School of Medicine, Shahed University, Tehran AD - School of Medicine, Shahed University, Tehran AD - Pathologist Y1 - 2006 PY - 2006 VL - 1 IS - 1 SP - 17 EP - 20 KW - Transitional Cell Carcinoma of the urinary bladder (TCC) KW - Grade KW - MDM2 oncoprotein DO - N2 - Objective: Transitional cell carcinoma (TCC) of the urinary bladder is the second common cancer of the genitourinary tract. Several parameters such as clinical and pathological parameters, molecular factors, and etc play a role in determination of prognosis and type of treatment. In this research study, the relationship between grade and MDM2 oncoprotein overexpression in TCC of bladder was evaluated. Material and Methods: This cross-sectional study was carried out on 75 paraffinembedded tissue blocks (deposited in Pathology Department) from patients with TCC of urinary bladder from 2002 to 2005. In this respect, 3 μm sections were taken from each block. In one of the sections, grade of tumor was determined according to WHO/ISUP criteria using hematoxylin-eosin staining method. In another section, percent of MDM2 positive cells were determined by microscopic observation of 100 cells in each section using immunohistochemical technique and specific antibody. Results: Mean of MDM2 positive cells was %9.08 ± 4.7. In addition, mean of MDM2 positive cells in low malignant potential tissue (grade I), low grade tissue (grade II), and high grade tissue (grade III) was %3.6 ± 2.2, %8.2 ±3.5, and %13.1 ± 3.4 respectively. Meanwhile, a significant relationship was found out between grade and overexpression of MDM2 oncoprotein (p = 0.012). Conclusion: Overexpression of MDM2 oncoprotein exhibits a direct relationship with grade of TCC of the urinary bladder. The obtained results were consistent with the most previous studies. Therefore, through performing studies with a greater follow-up and entailing a larger population of patients, overexpression of MDM2 oncoprotein could be used as a prognostic parameter in TCC of urinary bladder.   UR - https://ijp.iranpath.org/article_8895.html L1 - https://ijp.iranpath.org/article_8895_8196fbbb939eb38a86ccaf8681c6eb66.pdf ER -