TY - JOUR ID - 8340 TI - Immunofluorescence Pattern of Autoimmune Bullous Diseases in Iranian Patients JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Monsef, Alireza AU - Farshchian, Mahmood AU - Jafari, Mohammad AU - Farshchian, Mehdi AD - Dept. of Pathology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran AD - Dept. of Dermatology, School of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran Y1 - 2012 PY - 2012 VL - 7 IS - 4 SP - 231 EP - 235 KW - Immunofluorescence KW - Bullous Skin Diseases KW - Iran DO - N2 - Background & Objective: Autoimmune bullous diseases are associated with autoimmunity against structural components in the skin and mucous membranes. Autoantibodies are against the intercellular junctions in pemphigus disease and hemidesmosomal unchoring complex in pemphigiod diseases and epidermolysis bullosa aquisita. The tissue-bound and circulating serum autoantibodies can be detected with direct immunofluorescence (DIF) and indirect Immunofluorescence (IIF) tests. The aim of this study was to pinpoint the immunofluorescence pattern of Iranian patients with autoimmune bullous diseases. Methods: In a prospective case series study, sixteen patients with autoimmune disease enrolled in the study for two years. Perilesional skins and sera from the patients were used in DIF and IIF for detection of immunofluorescence pattern. Results:Out of 16 cases, 9 cases had pemphigus and 7 cases had bullous pemphigoid. All cases of pemphigus had positive DIF in intercellular region with lacelike pattern; IgG was detected in all cases, IgA in 1(11.1%) case and C3 in 3 (33.3%) cases. One (11.1%) case of pemphigus had positive IIF in intercellular region with lacelike pattern; circulating autoantibodies were IgG and IgA. All cases of bullous pemphigiod had positive DIF in dermal-epidermal Junction with linear pattern. IgG was detected in all cases, IgM in one (14.3%) case, and C3 in six (85.7%) cases. One (14.3%) case of bullous pemphoid had positive IIF in dermal-epidermal Junction with linear pattern; circulating autoantibody was IgG. Conclusion: Immunofluorescence tests are sensitive diagnostic methods for autoimmune bullous diseases. IIF positive cases in our study were lower compared to the previous reports.   UR - https://ijp.iranpath.org/article_8340.html L1 - https://ijp.iranpath.org/article_8340_637b9478edd93bb7a37797e8983dbcdf.pdf ER -